External irradiation models for intracranial 9L glioma studies

Abstract

PurposeRadiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.Materials and methods9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.ResultsIrradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.ConclusionsDelivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

Tumor eradication in rat glioma and bypass of immunosuppressive barriers using internal radiation with 188Re-lipid nanocapsules

“…Two groups were studied: a control group (n=6) and a treated one (n=8). We set the therapeutic dose at 16 Gy (2x8Gy) as the maximum tolerated dose (MTD) of 18 Gy (3x6Gy) proved to be effective in the 9L-glioma rat model [34].…”

Pre-clinical research in small animals using radiotherapy technology – a bidirectional translational approach

“…1A-B) [16,[60][61][62][63]. Bolus material or acrylic plates may be used to assure proper dose build up [60,61] or to shift the depth dose curve of an electron beam [63].…”

“…Image courtesy of Gabriel Charest; D: Tongue irradiation of a mouse using an orthovoltage X-ray unit [97]. Image courtesy of Wolfgang Dörr. accelerator, which are the multi-leaf-collimator (MLC) and electron applicators [16,[60][61][62][63]. Additionally, a customised block inserted into the accessory holder at the gantry or electron aperture made from MCP alloys (MCP Group, Tilly, Belgium) may be utilised [56][57][58].…”

Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers

“…Th e simultaneous irradiation of more than one rat was earlier reported by Vinchon-Petit et al (2010), but that was not a conformal method: the whole brains of four animals were treated with uniform 15 ϫ 15 cm radiation fi elds at a source-axis distance of 100 cm. In our own previous work, we achieved conformal, simultaneous irradiation limited to one region with the use of a special immobilization device and a BrainLab stereotactic system including a micro-multileaf collimator, but the frontal lobe Figure 7.…”