External gamma irradiation-induced effects in early-life stages of zebrafish, Danio rerio

Gagnaire, Béatrice; Cavalié, Isabelle; Pereira, Sandrine; Floriani, Magali; Dubourg, Nicolas; Camilleri, Virginie; Adam-Guillermin, Christelle

doi:10.1016/j.aquatox.2015.10.005

Cited by 44 publications

(72 citation statements)

References 35 publications

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“…Previous studies in zebrafish reported underlying molecular mechanisms responsible for adverse biological effects such as DNA damage [28,29], ROS, oxidative stress, apoptosis, bystander effects [30–32] and also genetic [32–34] and epigenetic changes [8] following exposure to ionizing radiation. However, most of the genetic responses were studied following acute exposures.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, in a previous study of hatching intervals following X-ray exposure during the blastula stage, earlier hatching was associated with low dose (25 mGy at 0.43 Gy/min), while higher doses (250–500 mGy) delayed the onset of hatching [37]. In addition, other studies report that both low and high doses had an accelerating effect on the hatching interval [28,32]. In the 15 mGy/h group, the total hatching was significantly decreased (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Collectively, these observations might indicate that the early developmental stages, prior to the hatching interval, are more sensitive to the effects of ionizing radiation, resulting in mortality. Other studies have reported no significant differences in embryo viability after receiving acute ionizing radiation doses ranging from 1–10 Gy [34,39], nor following continuous exposures up to 24 mGy/h (2280 mGy) [32], although the latter induced multiple deformities. Generally, the adverse effects on embryo development from the continuous exposure in the present study showed considerable variability in response to lower and higher doses, and in order to elucidate potential molecular mechanisms behind the observed effects, this variability was further studied by transcriptomics.…”

Section: Discussionmentioning

confidence: 99%

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Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression

et al. 2017

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Ionizing radiation from natural sources or anthropogenic activity has the potential to cause oxidative stress or genetic damage in living organisms, through the ionization and excitation of molecules and the subsequent production of free radicals and reactive oxygen species (ROS). The present work focuses on radiation-induced biological effects using the zebrafish (Danio rerio) vertebrate model. Changes in developmental traits and gene expression in zebrafish were assessed after continuous external gamma irradiation (0.4, 3.9, 15 and 38 mGy/h) with corresponding controls, starting at 2.5 hours post fertilization (hpf) and lasting through embryogenesis and the early larval stage. The lowest dose rate corresponded to recommended benchmarks at which adverse effects are not expected to occur in aquatic ecosystems (2–10 mGy/day). The survival observed at 96 hours post fertilization (hpf) in the 38 mGy/h group was significantly lower, while other groups showed no significant difference compared to controls. The total hatching was significantly lower from controls in the 15 mGy/h group and a delay in hatching onset in the 0.4 mGy/h group was observed. The deformity frequency was significantly increased by prolonged exposure duration at dose rates ≥ 0.4 mGy/h. Molecular responses analyzed by RNA-seq at gastrulation (5.5 hpf transcriptome) indicate that the radiation induced adverse effects occurred during the earliest stages of development. A dose-response relationship was found in the numbers of differentially regulated genes in exposure groups compared to controls at a total dose as low as 1.62 mGy. Ingenuity Pathway Analysis identified retinoic acid receptor activation, apoptosis, and glutathione mediated detoxification signaling as the most affected pathways in the lower dose rate (0.54 mGy/h), while eif2 and mTOR, i.e., involved in the modulation of angiogenesis, were most affected in higher dose rates (5.4 and 10.9 mGy/h). By comparing gene expression data, myc was found to be the most significant upstream regulator, followed by tp53, TNF, hnf4a, TGFb1 and cebpa, while crabp2b and vegfab were identified as most frequent downstream target genes. These genes are associated with various developmental processes. The present findings show that continuous gamma irradiation (≥ 0.54 mGy/h) during early gastrula causes gene expression changes that are linked to developmental defects in zebrafish embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression

et al. 2017

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“…A dose-response curve with a hormetic zone typically has a “J” shape or inverted “U” shape, and is thus nonlinear and biphasic, while a dose-response curve with an extra “subhormetic” zone in addition to a hormetic zone is “triphasic”. Gagnaire et al studied the effects of γ-irradiation in zebrafish larvae by examining physical parameters such as mortality and hatching rate as well as several biomarkers such as reactive oxygen species (ROS) production and detoxification enzyme [55]. Their results showed that IR-induced oxidative stress led to DNA damages [55].…”

Section: Ionizing Radiation Targeted Effects and Non-targeted Effmentioning

confidence: 99%

“…Gagnaire et al studied the effects of γ-irradiation in zebrafish larvae by examining physical parameters such as mortality and hatching rate as well as several biomarkers such as reactive oxygen species (ROS) production and detoxification enzyme [55]. Their results showed that IR-induced oxidative stress led to DNA damages [55]. Ryan et al identified a hyper-radiosensitive (HRS) response in the ZEB-2J (zebrafish cell line) cells upon γ-irradiation with a dose <0.5 Gy [56].…”

Section: Ionizing Radiation Targeted Effects and Non-targeted Effmentioning

confidence: 99%

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Kong

Cheng

2016

IJMS

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Exposure to ionizing radiations (IRs) is ubiquitous in our environment and can be categorized into “targeted” effects and “non-targeted” effects. In addition to inducing deoxyribonucleic acid (DNA) damage, IR exposure leads to epigenetic alterations that do not alter DNA sequence. Using an appropriate model to study the biological effects of radiation is crucial to better understand IR responses as well as to develop new strategies to alleviate exposure to IR. Zebrafish, Danio rerio, is a scientific model organism that has yielded scientific advances in several fields and recent studies show the usefulness of this vertebrate model in radiation biology. This review briefly describes both “targeted” and “non-targeted” effects, describes the findings in radiation biology using zebrafish as a model and highlights the potential of zebrafish to assess the epigenetic effects of IR, including DNA methylation, histone modifications and miRNA expression. Other in vivo models are included to compare observations made with zebrafish, or to illustrate the feasibility of in vivo models when the use of zebrafish was unavailable. Finally, tools to study epigenetic modifications in zebrafish, including changes in genome-wide DNA methylation, histone modifications and miRNA expression, are also described in this review.

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Cellular responses to ionizing radiation change quickly over time during early development in zebrafish

Honjo

Ichinohe

2019

Cell Biology International

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Animal cells constantly receive information about and respond to environmental factors, including ionizing radiation. Although it is crucial for a cell to repair radiation‐induced DNA damage to ensure survival, cellular responses to radiation exposure during early embryonic development remain unclear. In this study, we analyzed the effects of ionizing radiation in zebrafish embryos and found that radiation‐induced γH2AX foci formation and cell cycle arrest did not occur until the gastrula stage, despite the presence of major DNA repair‐related gene transcripts, passed on as maternal factors. Interestingly, P21/WAF1 accumulation began ∼6 h post‐fertilization, although p21 mRNA was upregulated by irradiation at 2 or 4 h post‐fertilization. These results suggest that the cellular responses of zebrafish embryos at 2 or 4 h post‐fertilization to radiation failed to overcome P21 protein accumulation and further signaling. Regulation of P21/WAF1 protein stabilization appears to be a key factor in the response to genotoxins during early embryogenesis.

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External gamma irradiation-induced effects in early-life stages of zebrafish, Danio rerio

Cited by 44 publications

References 35 publications

Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression

Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Cellular responses to ionizing radiation change quickly over time during early development in zebrafish

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