External Collar Inhibits Balloon-Induced Intimal Hyperplasia in Rabbits

Fogelstrand, Per; Risberg, B; Mattsson, Erney

doi:10.1159/000065548

Cited by 6 publications

(7 citation statements)

References 31 publications

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“…To further explore possible deleterious effects of marking pens on vein grafts we have studied the effect of crystal violet on venous cell migration, proliferation, and apoptosis. These are the major biological activities that contribute to intimal hyperplasia in both large and small animal models of vein graft healing 9–15 .…”

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confidence: 99%

Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue

Kikuchi

Kenagy

Wight

et al. 2016

Journal of Vascular Surgery

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Objective Markers containing dyes such as crystal violet (CAS 548-62-9) are routinely used on the adventitia of vein bypass grafts to avoid twisting during placement. Since little is known about how these dyes affect vein graft healing and function, we determined the effect of crystal violet on cell migration and proliferation, which are responses to injury after grafting. Methods Fresh human saphenous veins were obtained as residual specimens from leg bypass surgeries. Portions of the vein that had been surgically marked with crystal violet were analyzed separately from those that had no dye marking. In the laboratory, they were split into easily dissected inner and outer layers after removal of endothelium. This f cleavage plane was within the circular muscle layer of the media. Cell migration from explants was measured daily as either 1) % migration positive explants, which exclusively measures migration, or 2) the number of cells on the plastic surrounding each explant, which measures migration plus proliferation. Cell proliferation and apoptosis (Ki67 and TUNEL staining, respectively) were determined in dye-marked and unmarked areas of cultured vein rings. The dose-dependent effects of crystal violet were measured for cell migration from explants as well as proliferation, migration, and death of cultured outer layer cells. Dye was extracted from explants with ethanol and quantified by spectrophotometry. Results There was significantly less cell migration from visibly blue, compared to unstained, outer layer explants by both methods. There was no significant difference in migration from inner layer explants adjacent to blue-stained or unstained sections of vein, because dye did not penetrate to the inner layer. Ki67 staining of vein in organ culture, which is a measure of proliferation, progressively increased up to 6 days in non-blue outer layer and was abolished in the blue outer layer. Evidence of apoptosis (TUNEL staining) was present throughout the wall and not different in blue-stained and unstained vein wall segments. Blue outer layer explants had 65.9±8.0 ng dye/explant compared to 2.1±1.3 for non-blue outer layer explants. Dye applied in vitro to either outer or inner layer explants dose-dependently inhibited migration (IC50=8.5 ng/explant). The IC50s of crystal violet for outer layer cell proliferation and migration were 0.1 and 1.2 μg/ml, while the EC50 for death was between 1 and 10 μg/ml. Conclusion Crystal violet inhibits venous cell migration and proliferation indicating that alternative methods should be considered for marking vein grafts.

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confidence: 99%

Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue

Kikuchi

Kenagy

Wight

et al. 2016

Journal of Vascular Surgery

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“…The external shielding in the present study might have interfered with mechanical forces, such as shear or tensile stress, which are known to influence intimal hyperplasia [18 -20]. An external support in direct contact to the vessel wall has been shown to reduce intimal thickening in vein grafts [23,24] as well as in balloon-injured arteries [25]. However, the shielding in this study was not in close contact with the vessel wall.…”

Section: Discussionmentioning

confidence: 77%

Reduced neointima in vein grafts following a blockage of cell recruitment from the vein and the surrounding tissue

Fogelstrand

Österberg

Mattsson

2005

Cardiovascular Research

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Objective: Accumulation of intimal smooth muscle cells (SMC) is an important event in vein graft-stenosis. Different SMC sources have been reported, but their interrelations have been poorly studied. In a mouse vein graft model we investigated whether recipient-derived intimal SMCs are recruited from the surrounding tissue and whether blockage of SMC recruitment from the surrounding tissue and/or the donor vein will reduce neointimal formation. Methods: To detect recipient-derived cells, wild-type veins were implanted into ROSA26 transgenic mice. To block cell recruitment from the surrounding tissue, implanted veins were isolated with a tube-shaped plastic film. To exclude vein-derived cells in the neointimal formation, acellular veins were implanted. Results: In vein grafts isolated from the surrounding tissue the recipient contribution became minimal, but the total number of SMCs was not decreased. Acellular grafts contained an equal number of intimal SMCs as cellular controls after 4 weeks. Isolation of acellular grafts from the surrounding tissue decreased the number of intimal SMCs by 90%. Conclusions: Recipient-derived SMCs are mainly recruited from the surrounding tissue. Cell recruitment from either the vein or the surrounding tissue is enough to form a neointima. Therefore, a simultaneous inhibition of both these sources is needed to reduce accumulation of intimal SMCs.

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“…The restenotic luminal narrowing is due to constrictive remodeling and/or intimal hyperplasia (neointimal formation). The formation of intimal hyperplasia is commonly explained as a result of smooth muscle cell migration, proliferation, and deposition of extracellular matrix [21]. The best-studied model of neointimal formation is the response of the rat carotid artery to balloon angioplasty [22].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Biomechanical and Histological Properties in Dog Carotid Arteries Injured by Neointima or Intimal Thickening

Goto

Mizuno

Ono

et al. 2005

JJP

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A general formula (Oka and Azuma's equation) has been rigorously derived for the circumferential wall tension in a hollow cylindrical tube in equilibrium. To evaluate the validity and usefulness of Oka and Azuma's equation, T = P 1 × r 1 -P 2 × r 2 (T, circumferential wall tension; P 1 and P 2 , internal and external pressures of the tube; r 1 and r 2 , the corresponding internal and external radii), we experimentally investigated changes in circumferential wall tension of noninjured (control) and injured dog common carotid arteries by using a newly developed apparatus with a photo-and X-ray-sensitive image sensor. We also studied histological features of the control and injured arteries with special reference to the relation of biomechanical properties. Two types of animal models with injured arteriesballoon-induced neointima or external collar-induced intimal thickening-were adopted in the present study. In the control arteries, the circumferential wall tension was experimentally confirmed to change from negative to positive by an increase in intraluminal pressure ranging from 50 to 180 mmHg. The critical intraluminal pressure that produced 0 dyne/cm of the circumferential wall tension was around 135 mmHg. The activaAzuma and Oka [1] examined the validity of applying the law of Laplace to the mechanical equilibrium

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External Collar Inhibits Balloon-Induced Intimal Hyperplasia in Rabbits

Cited by 6 publications

References 31 publications

Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue

Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue

Reduced neointima in vein grafts following a blockage of cell recruitment from the vein and the surrounding tissue

Comparison of Biomechanical and Histological Properties in Dog Carotid Arteries Injured by Neointima or Intimal Thickening

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