External and internal detection of Nosema ceranae on honey bees using real-time PCR

Bourgeois, Lelania; Beaman, Lorraine D.; Holloway, Beth; Rinderer, Thomas E.

doi:10.1016/j.jip.2012.01.002

Cited by 20 publications

(11 citation statements)

References 12 publications

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“…DNA contamination from the external parts of the body. In a recent study, N. ceranae has been detected in wash lysates (Bourgeois et al, 2012), suggesting that our method of using a bleach was effective at removing Nosema spp. spores externally.…”

Section: Genomic Dna (Gdna) Extractionmentioning

confidence: 66%

Low natural levels of Nosema ceranae in Apis mellifera queens

Traver

Fell

2012

Journal of Invertebrate Pathology

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Section: Genomic Dna (Gdna) Extractionmentioning

confidence: 66%

Low natural levels of Nosema ceranae in Apis mellifera queens

Traver

Fell

2012

Journal of Invertebrate Pathology

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“…In contrast with the cage study of Fontbonne and colleagues (), field experiments of natural infection found differential levels of N. ceranae between molecularly identified Russian (M‐lineage) and Italian bees (C‐lineage) in the USA (Bourgeois et al ., ), and between Africanized (A‐lineage) and Italian bees in Uruguay (Mendoza et al ., ), with Italian bees seemingly more susceptible in both cases. Furthermore, the results of Bourgeois and colleagues () provide the first evidence for genetic variation in resistance to N. ceranae in the Russian stock.…”

Section: What Are the Major Effects Of N Ceranae On Honey Bees?mentioning

confidence: 91%

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Martín-Hernández

Bartolomé

Chejanovsky

et al. 2018

Environmental Microbiology

159

118

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Nosema ceranae is a hot topic in honey bee health as reflected by numerous papers published every year. This review presents an update of the knowledge generated in the last 12 years in the field of N. ceranae research, addressing the routes of transmission, population structure and genetic diversity. This includes description of how the infection modifies the honey bee's metabolism, the immune response and other vital functions. The effects on individual honey bees will have a direct impact on the colony by leading to losses in the adult's population. The absence of clear clinical signs could keep the infection unnoticed by the beekeeper for long periods. The influence of the environmental conditions, beekeeping practices, bee genetics and the interaction with pesticides and other pathogens will have a direct influence on the prognosis of the disease. This review is approached from the point of view of the Mediterranean countries where the professional beekeeping has a high representation and where this pathogen is reported as an important threat.

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“…; Bourgeois et al . ). Both of these methods require trained personnel and sophisticated equipment and therefore cannot be readily adapted by the bee‐keepers.…”

Section: Introductionmentioning

confidence: 97%

“…Currently, there are two main approaches for detection of Nosema in bees: microscopic examinations (Cantwell 1970) and DNA amplification (Fries and Ekbom 1984;Fries 1989;Gatehouse and Malone 1998;Webster et al 2004;Bourgeois et al 2012). Both of these methods require trained personnel and sophisticated equipment and therefore cannot be readily adapted by the bee-keepers.…”

Section: Introductionmentioning

confidence: 99%

A serological method for detection of Nosema ceranae

2012

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Aims: We developed a new method for detection of the intracellular parasite, Nosema ceranae, one of the most economically devastating pathogens of the honeybee. Methods and Results: The SWP-32 antibody was used for the development of an enzyme-linked immunosorbent assay (ELISA). We also compared the efficiency of this ELISA to microscopy and quantitative real-time (qRT) PCR, the methods currently in use. Conclusions: ELISA is comparable in sensitivity with the qRT-PCR, less expensive and faster. When this method is commercialized and made available to bee-keepers, it will allow them to make informed decisions for the application of in-hive chemicals. Hence, bee-keepers may be able to determine when treatments for control of N. ceranae are unnecessary and reduce the cost, time and possible side effects of these treatments. Significance and Impact of the Study: This assay provides the first serological method for detection of N. ceranae in bee colonies, which is as sensitive as DNA amplification. It can be easily adopted for both laboratory and field applications.

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External and internal detection of Nosema ceranae on honey bees using real-time PCR

Cited by 20 publications

References 12 publications

Low natural levels of Nosema ceranae in Apis mellifera queens

Low natural levels of Nosema ceranae in Apis mellifera queens

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

A serological method for detection of Nosema ceranae

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