Extent of the mouse t complex and its inversions shown by in situ hybridization

Lyon, Mary F.; Zenthon, J; Evans, E. P.; Burtenshaw, M. D.; Willison, Keith R.

doi:10.1007/bf00395134

Cited by 32 publications

(11 citation statements)

References 32 publications

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“…Additionally, we find strong statistical support for identical marker order on the Mmc and Mmm genetic linkage maps. Although small inversions (Lyon et al 1988), CNVs (Cheung et al 2003;She et al 2008), and insertions/deletions (Akagi et al 2010) segregate among house mice, such small-scale structural changes are not expected to exert systematic effects on the number or distribution of recombination events in a genome. Intervals showing map length differences between our two crosses do not show higher levels of CNV compared with genomic regions with no map length differences (Supplemental Table 3).…”

Section: Detection Of Map Length Differencesmentioning

confidence: 99%

Extensive recombination rate variation in the house mouse species complex inferred from genetic linkage maps

Dumont¹,

White²,

Steffy³

et al. 2010

Genome Res.

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The rate of recombination is a key genomic parameter that displays considerable variation among taxa. Species comparisons have demonstrated that the rate of evolution in recombination rate is strongly dependent on the physical scale of measurement. Individual recombination hotspots are poorly conserved among closely related taxa, whereas genomic-scale recombination rate variation bears a strong signature of phylogenetic history. In contrast, the mode and tempo of evolution in recombination rates measured on intermediate physical scales is poorly understood. Here, we conduct a detailed statistical comparison between two whole-genome F 2 genetic linkage maps constructed from experimental intercrosses between closely related house mouse subspecies (Mus musculus). Our two maps profile a common wild-derived inbred strain of M. m. domesticus crossed to distinct wild-derived inbred strains representative of two other house mouse subspecies, M. m. castaneus and M. m. musculus. We identify numerous orthologous genomic regions with significant map length differences between these two crosses. Because the genomes of these recently diverged house mice are highly collinear, observed differences in map length (centimorgans) are suggestive of variation in broadscale recombination rate (centimorgans per megabase) within M. musculus. Collectively, these divergent intervals span 19% of the house mouse genome, disproportionately aggregating on the X chromosome. In addition, we uncover strong statistical evidence for a large effect, sex-linked, site-specific modifier of recombination rate segregating within M. musculus. Our findings reveal considerable variation in the megabase-scale recombination landscape among recently diverged taxa and underscore the continued importance of genetic linkage maps in the post-genome era.

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Section: Detection Of Map Length Differencesmentioning

confidence: 99%

Extensive recombination rate variation in the house mouse species complex inferred from genetic linkage maps

Dumont¹,

White²,

Steffy³

et al. 2010

Genome Res.

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“…Classical cytogenetic investigations failed to detect these inversions (38,39) and have been generally unsuccessful in discovering rearrangements among species of Mus (40). However, inversions have been visualized by comparative in situ hybridizations with probes for loci within the proximal and distal inversions (41). With the advent of pulsed-field gel electrophoresis, it should now be possible to survey populations for the presence of the proximal inversion, as well as the other inversions, without the need for genetic crosses.…”

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confidence: 99%

Evolution of mouse chromosome 17 and the origin of inversions associated with t haplotypes.

Hammer

Schimenti

Silver

1989

Proc. Natl. Acad. Sci. U.S.A.

172

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Mouse t haplotypes are variant forms of chromosome 17 that exist at high frequendes in worldwide populations of several species of house mouse. They are known to differ from wild-type chromosomes with respect to two relative inversions referred to as proximal and distal. An untested assumption has been that these two inversions originated in the chromosomal lineage lading to present-day t haplotypes. To investigate the evolutionary origins of these inversions and the possibility of additional inversions, interspecific crosses were performed between Mus spretus or Mus abbotti and laboratory strains of Mus domesticus that carried wild-type and t haplotypes forms of chromosome 17. The results provide evidence for the existence of two additional nonoverlapping inversions-one between the proximal and distal inversions and one between the centromere and the proximal inversion. These four inversions span nearly the entire region of t haplotype recombination suppression. Considering the distribution of these inversions among the species studied as well as the organization of the D17Leh66 family of DNA elements, we infer that the proximal inversion occurred on the lineage leading to the common ancestor ofM. domesticus and M. abbott, and that the other three inversions occurred on the separate lineage leading to present-day t haplotypes.Alternative models for the evolution of t haplotypes are discussed in light of these fndings.Two forms of the proximal region of mouse chromosome 17 are found in natural populations of house mice. One form is considered wild type (+) and the other is known as a t haplotype (t) (1, 2). A t haplotype is able to propagate itself at the expense of its wild-type meiotic partner, in a clear departure from Mendel's first law. The integrity of a complete t haplotype is maintained by a suppression of recombination along its 15-centimorgan (cM) length from the DI7Leh48 locus to the H-2 complex (Fig. 1). These chromosomes have been identified in several house mouse species including Mus domesticus, Mus musculus (3), Mus molossinus (4), and Mus bactrianus (unpublished data). In surveys of M. domesticus from many geographical locations, t haplotypes have been found at frequencies between 10%o and 20%1o, even though they carry genes that cause homozygous male sterility, and some also carry embryonic lethal mutations (5, 6).The major selective force driving t haplotypes in populations is the high ratio of transmission from +/t heterozygous males (7). Genetic experiments have demonstrated the existence of at least five independent loci involved in this transmission ratio distortion (TRD) (refs. 8 and 9; see Fig. 1). In general, only t haplotypes with a complete set of TRD loci are transmitted at high ratios, and only high-ratio t haplotypes survive for significant periods of time in natural populations (7). Because the TRD loci are spread across a 15-cM chromosomal region, the continued presence of t haplotypes in populations depends as much on recombination suppression as on TRD.The discovery o...

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“…The t complex is the name given to the rearranged region. It contains about 100 genes (39), including several genes expressed in the testes whose products show t-allele-specific alterations.…”

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confidence: 99%

The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes.

Ursic¹,

Culbertson²

1991

Mol. Cell. Biol.

163

112

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A Saccharomyces cerevisiae homolog to Drosophila melanogaster and mouse Tcp-1 encoding taiDless complex polypeptide 1 (TCP1) has been identified, sequenced, and mapped. The mouse t complex and many of its unusual properties have attracted the attention of geneticists for nearly 60 years (11,12,20,60). t alleles were first discovered by their interaction with a dominant T-locus mutation to produce a tailless phenotype in double heterozygous T/t animals. Heterozygous T/+ animals have a short tail, and homozygous T/T animals are embryonic lethals. Homozygous tx/tY males are sterile (37, 38), and heterozygous +/t animals have tails of normal length and are visually indistinguishable from normal wild-type +/+ animals. Compared with wild-type laboratory chromosome 17, the principal variation in t chromosomes consists of at least four inversions of about 1% of the mouse genome (30 Mbs of DNA) (21, 23). The t complex is the name given to the rearranged region. It contains about 100 genes (39), including several genes expressed in the testes whose products show t-allele-specific alterations.t chromosomes confer a number of effects, including disturbances of embryonic development, alterations of sperm differentiation and function, and transmission ratio distortion. While transmission of the t complex through females is normal, heterozygous t/+ males transmit the t-bearing chromosome to their progeny in excess of the Mendelian expectation. Genetic analysis of transmission ratio distortion suggests that it depends on the action of up to four distorter loci (Tcd-J to Tcd4) as well as a responder locus (Tcr) (2, 36-38, 56, 63). The molecular basis of transmission ratio distortion remains unknown.A number of candidate genes for the distorter and responder loci, including Tcp-1, have been isolated from the t complex (75). Tailless complex polypeptide 1 (TCP-1), a protein of 57 kDa, is expressed in large amounts during * Corresponding author.

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Extent of the mouse t complex and its inversions shown by in situ hybridization

Cited by 32 publications

References 32 publications

Extensive recombination rate variation in the house mouse species complex inferred from genetic linkage maps

Extensive recombination rate variation in the house mouse species complex inferred from genetic linkage maps

Evolution of mouse chromosome 17 and the origin of inversions associated with t haplotypes.

The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes.

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