Extensively Drug-Resistant Salmonella typhi Infection: From Pill to Scalpel

Rahim, Fawad; Amin, Sakib Bin; Noor, Mohammad; Hadi, Khushal Nadir; Aftab, Sana

doi:10.7759/cureus.25840

Cited by 1 publication

(2 citation statements)

References 15 publications

(20 reference statements)

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“…Salmonella is an important foodborne pathogen and becomes a serious threat to public health . Eating Salmonella contaminated food may cause vomiting, diarrhea, abdominal pain, and even death . It is estimated that there are 1.35 million illnesses, 26,500 hospitalizations, and 420 deaths caused by Salmonella every year in the United States .…”

Section: Introductionmentioning

confidence: 99%

“…1 Eating Salmonella contaminated food may cause vomiting, diarrhea, abdominal pain, and even death. 2 It is estimated that there are 1.35 million illnesses, 26,500 hospitalizations, and 420 deaths caused by Salmonella every year in the United States. 3 Effective and rapid detection of Salmonella could reduce Salmonella contaminants and prevent foodborne disease outbreaks, especially in early stage of pathogen infection.…”

Section: ■ Introductionmentioning

confidence: 99%

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Dual-Functional Tetrahedron Multivalent Aptamer Assisted Amplification-Free CRISPR/Cas12a Assay for Sensitive Detection of Salmonella

Qiao,

Xue,

Sun

et al. 2023

J. Agric. Food Chem.

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Salmonellosis continues to impose a significant economic burden globally. Rapid and sensitive detection of Salmonella is crucial to preventing the outbreaks of foodborne illnesses, yet it remains a formidable challenge. Herein, a dual-functional tetrahedron multivalent aptamer assisted amplification-free CRISPR/Cas12a assay was developed for Salmonella detection. In the system, the aptamer was programmatically assembled on the tetrahedral DNA nanostructure to fabricate a multivalent aptamer (TDN-multiApt), which displayed a 3.5-fold enhanced avidity over the monovalent aptamer and possessed four CRISPR/Cas12a targeting fragments to amplify signal. Therefore, TDN-multiApt could directly activate Cas12a to achieve the second signal amplification without any nucleic acid amplification. By virtue of the synergism of high avidity and cascaded signal amplifications, the proposed method allowed the ultrasensitive detection of Salmonella as low as 7 cfu mL −1 . Meanwhile, this novel platform also exhibited excellent specificity against target bacteria and performed well in the detection of various samples, indicating its potential application in real samples.

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Section: Introductionmentioning

confidence: 99%