Extensive screening of sturgeon genomes by random screening techniques revealed no sex-specific marker

Wuertz, Sven; Gaillard, Sandrine; Barbisan, Federica; Carle, Serge; Congiu, Leonardo; Forlani, Anna; Aubert, Josiane; Kirschbaum, Frank; Tosi, Elisabetta; Zane, Lorenzo; Grillasca, Joël-Paul

doi:10.1016/j.aquaculture.2006.03.042

Cited by 63 publications

(44 citation statements)

References 23 publications

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“…However, sturgeon geneticists have failed to find sex-determining genes despite using a plethora of molecular approaches (Hett and Ludwig 2005;Wuertz et al 2006;Keyvanshokooh et al 2007;McCormick et al 2008). Similarly, the research described herein provides no evidence for a master gene whose presence or absence determines lake sturgeon sex.…”

Section: Sex-determining Genesmentioning

confidence: 53%

“…have failed to identify master sex-determining genes in a variety of fishes, including sturgeon (Wuertz et al 2006;Keyvanshokooh et al 2007;McCormick et al 2008). Our strategy was to diminish the overall complexity of the lake sturgeon genome by sequencing cDNA instead of genomic DNA.…”

Section: Sex-determining Genesmentioning

confidence: 99%

“…DNA sexing assays have proven invaluable in the conservation of wildlife and could be valuable in fish conservation as well, where they could be used to determine sex ratios at stocking as well as sex-specific mortality rates (DeWoody et al 2010). However, the search for sturgeon sex determining genes has been unsuccessful using traditional molecular approaches such as AFLPs, RAPDs, and subtractive hybridization (Wuertz et al 2006;Keyvanshokooh et al 2007;McCormick et al 2008), probably because the sturgeon genome is polyploid and most loci are inherited tetrasomically or octasomically (Blacklidge and Bidwell 1993;Ludwig et al 2001;DeWoody et al 2010). Here, we use next-generation 454 sequencing of gonad transcriptomes to annotate lake sturgeon genes involved in sex determination as well as genes from xenobiotic organisms.…”

Section: Introductionmentioning

confidence: 97%

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Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

2010

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Modern pyrosequencing has the potential to uncover many interesting aspects of genome evolution, even in lineages where genomic resources are scarce. In particular, 454 pyrosequencing of nonmodel species has been used to characterize expressed sequence tags, xenobiotics, gene ontologies, and relative levels of gene expression. Herein, we use pyrosequencing to study the evolution of genes expressed in the gonads of a polyploid fish, the lake sturgeon (Acipenser fulvescens). Using 454 pyrosequencing of transcribed genes, we produced more than 125 MB of sequence data from 473,577 high-quality sequencing reads. Sequences that passed stringent quality control thresholds were assembled into 12,791 male contigs and 32,629 female contigs. Average depth of coverage was 4.2 x for the male assembly and 5.5x for the female assembly. Analytical rarefaction indicates that our assemblies include most of the genes expressed in lake sturgeon gonads. Over 86,700 sequencing reads were assigned gene ontologies, many to general housekeeping genes like protein, RNA, and ion binding genes. We searched specifically for sex determining genes and documented significant sex differences in the expression of two genes involved in animal sex determination, DMRT1 and TRA-1. DMRT1 is the master sex determining gene in birds and in medaka (Oryzias latipes) whereas TRA-1 helps direct sexual differentiation in nematodes. We also searched the lake sturgeon assembly for evidence of xenobiotic organisms that may exist as endosymbionts. Our results suggest that exogenous parasites (trematodes) and pathogens (protozoans) apparently have infected lake sturgeon gonads, and the trematodes have horizontally transferred some genes to the lake sturgeon genome.

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Section: Sex-determining Genesmentioning

confidence: 53%

Section: Sex-determining Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

2010

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“…Attempts have failed to identify a master sex-determining gene in a variety of fishes, including sturgeons (32,43,64). We sequenced different sexes at different developmental stages and tried to identify sex-related genes in Russian sturgeon.…”

Section: Discussionmentioning

confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

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Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads. Physiol Genomics 48: 464 -476, 2016. First published May 6, 2016 doi:10.1152/physiolgenomics.00113.2015.-As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated Ͼ60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.

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“…It should be noted that the attempts to detect a molecular marker for the identification of sex in several fish species such as Nile tilapia Oreochromis niloticus (McConnell et al, 1996;Bardakci, 2000), Atlantic salmon Salmo salar (McGowan and Davidson, 1998), the green spotted pufferfish Tetraodon nigroviridis (Li et al, 2002), and sturgeons Acipenser spp. (Wuertz et al, 2006) failed. In the present study, we screened only 47 RAPD primers and, despite observed DNA marker polymorphisms, did not identify any sex-related markers.…”

Section: Discussionmentioning

confidence: 90%

Progress in Studies on Hormonal Sex Reversal and Genetic Sex Control in Black Crappie

Cuevas‐Uribe

Gomelsky

Mims

et al. 2009

Reviews in Fisheries Science

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Sex control can solve the problem of stunted black crappie populations in small impoundments. The main objectives of the present study were (1) to identify sex-reversed males of black crappie from a previously obtained androgen-treated group using test crosses, and (2) to develop broodstock of sex-reversed males by masculinization of fish from those crosses. An additional objective of the study was to try to identify sex-specific RAPD markers, which might be used for identification of sex-reversed males. The progenies resulting from test crosses were divided into two groups: Group I (control) fish were raised without hormonal treatment, while Group II fish were subjected to androgen (MT) treatment. Seven progenies were obtained from the cross of preliminary androgen-treated males with normal females. Six of seven progenies had sex ratio in Groups I close to 1:1, and one progeny consisted of females only. This shows that only one male, which generated this progeny, was a sex-reversed homogametic fish (XX) while the other males were heterogametic normal (XY) fish. In Group II the androgen 17α-methyltestosterone (MT) was orally administered to crappie with an artificial diet (30 mg/kg) for 40 days beginning 35 days post hatching; androgen-treated groups consisted of 95-100% males. Sex-specific random-amplified polymorphic DNA markers were not identified in black crappie.

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Extensive screening of sturgeon genomes by random screening techniques revealed no sex-specific marker

Cited by 63 publications

References 23 publications

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Progress in Studies on Hormonal Sex Reversal and Genetic Sex Control in Black Crappie

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