Extensive genomic diversity among Mycobacterium marinum strains revealed by whole genome sequencing

Abstract

33Mycobacterium marinum is the causative agent for the tuberculosis-like disease 34 mycobacteriosis in fish and skin lesions in humans. Ubiquitous in its geographical 35 distribution, M. marinum is known to occupy diverse fish as hosts. However, information 36 about its genomic diversity is limited. Here, we provide the genome sequences for 15 M. 37 marinum strains isolated from infected humans and fish. Comparative genomic analysis of 38 these and four available genomes of the M. marinum strains M, E11, MB2 and Europe 39 reveal high genomic diversity among the strains, leading to the conclusion that M. 40 marinum should be divided into two different clusters, the "M"-and the "Aronson"-type. 41We suggest that these two clusters should be considered, if not two separate species, at 42 least two M. marinum subspecies. Our data also show that the M. marinum pan-genome for

Comparative genomic analysis of Mycobacterium intracellulare: implications for clinical taxonomic classification in pulmonary Mycobacterium avium-intracellulare complex disease

“…Recent advances in genome sequencing technology have enabled the comparison of clinically-isolated strains at the whole genome level and this has revealed that some mycobacterial species have open pan-genomes [6][7][8][9]. In M. avium, the genomic diversity is classified into several subspecies, including subspecies avium, hominissuis, paratuberculosis, and silvaticum [10].…”

Temporal genome-wide fitness analysis ofMycobacterium marinumduring infection reveals genetic requirement for virulence and survival in amoebae and microglial cells

“…Surprisingly, another Tn-Seq conducted on the M. marinum E11 strain showed only 6% of essential genes (Weerdenburg et al, 2015). The difference between the two studies is probably due to their genomic diversity, a recent comparative genomic analysis revealed that M. marinum strains should be divided into two different clusters, the "M"-and the "Aronson"-type (Das et al, 2018). Among the essential genes identified in M. marinum M, 237 orthologues were found in M. marinum E11 (37.3%) (Weerdenburg et al, 2015), and 311 orthologues in M. tuberculosis (44.4%) (DeJesus, Gerrick, et al, 2017).…”