Extensive Genomic Diversity among Bovine-Adapted Staphylococcus aureus: Evidence for a Genomic Rearrangement within CC97

Budd, Kathleen E.; McCoy, Finola; Monecke, Stefan; Cormican, Paul; Mitchell, Joan S.; Keane, Orla M.

doi:10.1371/journal.pone.0134592

Cited by 35 publications

(67 citation statements)

References 56 publications

(60 reference statements)

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“…CC1 is a common lineage among humans including both MSSA and community-acquired (CA-) MRSA; and CC1 is also frequently found in cattle [114,115]. A strain that closely resembled the MRSA from rooks was frequently found among humans in Romania [116].…”

Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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“…Both sequence types have been reported extensively from bovine mastitis cases in many countries including, but not limited to, Brazil, Chile, Italy, Japan, Norway, Spain, the Netherlands and the United States (Smith et al, 2005; Rabello et al, 2007; Hata et al, 2010). The expansive distribution of this successful lineage suggests that the strains are specifically adapted to the bovine host (Smith et al, 2005; Budd et al, 2015). …”

Section: Discussionmentioning

confidence: 99%

“…Several epidemiological studies have suggested that a few specialized S. aureus strains are responsible for the majority of bovine intramammary infections (IMIs) worldwide (Kapur et al, 1995; Zadoks et al, 2002; Sakwinska et al, 2011; Budd et al, 2015). However, the occurrence of herd-specific strains has also been reported (Joo et al, 2001; Sommerhäuser et al, 2003; Rabello et al, 2007; Oliveira et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Staphylococcus aureus Isolated from Bovine Mastitis and Close Human Contacts in South African Dairy Herds: Genetic Diversity and Inter-Species Host Transmission

2017

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Staphylococcus aureus is one of the most common etiological agents of contagious bovine mastitis worldwide. The purpose of this study was to genetically characterize a collection of S. aureus isolates (bovine = 146, human = 12) recovered from cases of bovine mastitis and nasal swabs of close human contacts in the dairy environment. Isolates were screened for a combination of clinically significant antimicrobial and virulence gene markers whilst the molecular epidemiology of these isolates and possible inter-species host transmission was investigated using a combination of genotyping techniques. None of the isolates under evaluation tested positive for methicillin or vancomycin resistance encoding genes. Twenty seven percent of the bovine S. aureus isolates tested positive for one or more of the pyrogenic toxin superantigen (PTSAg) genes with the sec and sell genes predominating. Comparatively, 83% of the human S. aureus isolates tested positive for one or more PTSAg genes with a greater variety of genes being detected. Genomic DNA macrorestriction followed by pulsed-field gel electrophoresis (PFGE) of the bovine isolates generated 58 electrophoretic patterns which grouped into 10 pulsotypes at an 80% similarity level. The majority of the bovine isolates, 93.2% (136/146), clustered into four major pulsotypes. Seven sequence types (ST) were identified among the representative bovine S. aureus isolates genotyped, including: ST8 (CC8), ST97 (CC97), ST351 (CC705), ST352 (CC97), ST508 (CC45), ST2992 (CC97) and a novel sequence type, ST3538 (CC97). Based on PFGE analysis, greater genetic diversity was observed among the human S. aureus isolates. Bovine and human isolates from three sampling sites clustered together and were genotypically indistinguishable. Two of the isolates, ST97 and ST352 belong to the common bovine lineage CC97, and their isolation from close human contacts suggests zoonotic transfer. In the context of this study, the third isolate, ST8 (CC8), is believed to be a human clone which has transferred to a dairy cow and has subsequently caused mastitis. The detection of indistinguishable S. aureus isolates from bovine and human hosts at three of the sampling sites is suggestive of bacterial transmission and supports the need for vigilant monitoring of staphylococcal populations at the human-animal interface.

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“…Within each of the major bovine IMI pathogen species, a diversity of strains and lineages (see Table 1) exists, each with a potentially unique repertoire of virulence factors and immune-stimulatory antigens (Budd et al, 2015;Hossain et al, 2015;Keane, 2016). Strain typing of E. coli, Strep.…”

Section: Genomics Of Imi Pathogensmentioning

confidence: 99%

“…uberis considered environmental pathogens (Bradley, 2002). More recently it has been recognized that some IMI pathogens may be capable of both contagious and environmental transmission and that the mode of transmission may be associated with the strain rather than species of pathogen (Zadoks et al, 2000;Budd et al, 2015;Davies et al, 2016). Prevention and management of contagious mastitis relies on the implementation of several well-characterized infection control strategies such as those outlined in the National Mastitis Council's "5-point" plan (NMC, 2011), later extended to a "10-point" plan.…”

Section: Introductionmentioning

confidence: 99%

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

Keane

2019

Journal of Dairy Science

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Intramammary infection (IMI) is one of the most costly diseases to the dairy industry. It is primarily due to bacterial infection and the major intramammary pathogens include Escherichia coli, Streptococcus uberis, and Staphylococcus aureus. The severity and outcome of IMI is dependent on several host factors including innate host resistance, energy balance, immune status, parity, and stage of lactation. Additionally, the infecting organism can influence the host immune response and progression of disease. It is increasingly recognized that not only the infecting pathogen species, but also the strain, can affect the transmission, severity, and outcome of IMI. For each of 3 major IMI-associated pathogens, S. aureus, Strep. uberis, and E. coli, specific strains have been identified that are adapted to the intramammary environment. Strain-dependent variation in the host immune response to infection has also been reported. The diversity of strains associated with IMI must be considered if vaccines effective against the full repertoire of mammary pathogenic strains are to be developed. Although important advances have been made recently in understanding the molecular mechanism underpinning strain-specific virulence, further research is required to fully elucidate the cellular and molecular pathogenesis of mammary adapted strains and the role of the strain in influencing the pathophysiology of infection. Improved understanding of molecular pathogenesis of strains associated with bovine IMI will contribute to the development of new control strategies, therapies, and vaccines. The development of enabling technologies such as pathogenomics, transcriptomics, and proteomics can facilitate system-level studies of strain-specific molecular pathogenesis and the identification of key mediators of host-pathogen interactions.

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Extensive Genomic Diversity among Bovine-Adapted Staphylococcus aureus: Evidence for a Genomic Rearrangement within CC97

Cited by 35 publications

References 56 publications

Diversity of Staphylococcus aureus Isolates in European Wildlife

Diversity of Staphylococcus aureus Isolates in European Wildlife

Molecular Characterization of Staphylococcus aureus Isolated from Bovine Mastitis and Close Human Contacts in South African Dairy Herds: Genetic Diversity and Inter-Species Host Transmission

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

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