Extensive database of liquid phase diffusion coefficients of some frequently used test molecules in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography

Song, Heping; Vanderheyden, Yoachim; Adams, Erwin; Desmet, Gert; Cabooter, Deirdre

doi:10.1016/j.chroma.2016.05.054

Cited by 34 publications

(27 citation statements)

References 36 publications

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“…A series of reference compounds were analysed to further validate the experimental set up. The values obtained for toluene (9.06 × 10 −10 m 2 /s), ascorbic acid (8.68 × 10 −10 m 2 /s), phenol (1.05 × 10 −9 m 2 /s) and thiourea (1.40 × 10 −9 m 2 /s) were consistent with reported data . Having demonstrated the validity of the experimental set‐up, phytocannabinoid D m values were calculated as a function of temperature (30, 40, 50°C).…”

Section: Resultssupporting

confidence: 86%

“…The observed range 0.854–0.912 × 10 −9 m 2 /s at 50°C (Tables and S2), while narrow, was reasonable when the structural similarity of all the phytocannabinoids was considered. The absolute phytocannabinoid D m values reported herein are consistent with recently published D m values for reference compounds with analogous lipophilicity and size . Having these physical constants will facilitate future expansion of the developed method to include additional phytocannabinoids, or potentially synthetic cannabinoids.…”

Section: Resultssupporting

confidence: 86%

“…To calculate the reduced linear velocity, it was necessary to know the phytocannabinoid bulk diffusion coefficients ( D m ; Table , Table S2). The D m values were calculated using the Taylor–Aris open tubular method summarised by Song et al . Since the capillary dimensions used in the present study were different than Song et al ., the contributions of axial diffusion (equations –) and radial concentration equilibration (equation ) to the observed band‐broadening were evaluated:

σ_{normalt, diff}^{2} ≪ σ_{normalt, Taylor - Aris}^{2}

σ_{normalt, diff}^{2} = \frac{2 \times D_{m} \times t_{r}}{u^{2}}

σ_{normalt, Taylor - Aris}^{2} = {(\frac{FWHM}{2.35})}^{2}

L ≫ \frac{d_{t}^{2} \times u}{60 \times D_{m}}

with

σ_{normalt, diff}^{2}

the peak variance due to axial diffusion,

σ_{normalt, Taylor - Aris}^{2}

the standard Gaussian peak variance, t r the retention time of the solute in the open tubular capillary, u the linear velocity, L the length and

d_{t}^{2}

the diameter of the capillary.…”

Section: Resultssupporting

confidence: 84%

“…Bulk molecular diffusion coefficients ( D m values) were calculated at 30, 40 and 50°C (depending on the column and UPLC used, Table ) with the open tubular method described by Song et al . using equation :

D_{m} = \frac{d_{t}^{2} \times t}{96 \times σ_{t}^{2}}

where d t is the capillary tube diameter, t is the residence time in the capillary and σ t is the chromatographic peak variance (Table S2).…”

Section: Methodsmentioning

confidence: 99%

“…Bulk molecular diffusion coefficients (D m values) were calculated at 30, 40 and 50°C (depending on the column and UPLC used, Table 1) with the open tubular method described by Song et al 19 using equation 2:…”

Section: Column Metricsmentioning

confidence: 99%

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