Extension of coronary artery disease is associated with increased IL-6 and decreased adiponectin gene expression in epicardial adipose tissue

Eiras, Sonia; Teijeira-Fernández, Elvis; Shamagian, Lilian Grigorian; Fernández, Ángel L.; Vázquez-Boquete, Ángel; González-Juanàtey, José Ramón

doi:10.1016/j.cyto.2008.05.006

Cited by 116 publications

(85 citation statements)

References 29 publications

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“…Like other adipose tissues, EAT is now recognized as an endocrine organ, and its proximity to myocardium and coronary arteries suggests the possibility of paracrine action on these structures. In fact, in patients with coronary artery disease (CAD) (5,12) and hypertension (32), EAT produces lower levels of the anti-inflammatory adipokine adiponectin and higher levels of the proinflammatory cytokines IL-6 and leptin than does the EAT of non-CAD or nonhypertensive patients undergoing cardiac surgery, whereas the levels of adiponectin in SAT do not differ between hypertensive and nonhypertensive patients (32), and proinflammatory cytokines are higher in the EAT than the SAT of CAD patients (20).…”

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confidence: 99%

Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress

Salgado-Somoza¹,

Teijeira-Fernández

Fernández

et al. 2010

American Journal of Physiology-Heart and Circulatory Physiology

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Salgado-Somoza A, Teijeira-Fernández E, Fernández AL, González-Juanatey JR, Eiras S. Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress. Am J Physiol Heart Circ Physiol 299: H202-H209, 2010. First published April 30, 2010; doi:10.1152/ajpheart.00120.2010.-Epicardial adipose tissue (EAT) is an endocrine organ adjacent to coronary arteries and myocardium without anatomy barriers. Locally produced adipokines may reflect or affect to cardiovascular physiology and pathology. Our aim was to study the protein expression profiles of EAT and subcutaneous adipose tissue (SAT) to identify local candidate molecules characterizing EAT in patients with cardiovascular disease. EAT and SAT samples were collected from 55 patients undergoing heart surgery. Proteins from these tissues were separated by two-dimensional (2D) gel electrophoresis, and differences between them were identified by MALDI-TOF/TOF spectra. Differences in protein levels were further investigated by real-time RT-PCR and Western blots, and production of reactive oxygen species (ROS) in EAT and SAT was evaluated by nitroblue tetrazolium chloride assays. ROS production was higher in EAT than SAT. We have found mRNA differences for catalase, glutathione S-transferase P, and protein disulfide isomerase, and 2D Western blots additionally showed post-translational differences for phosphoglycerate mutase 1; all four are related to oxidative stress pathways. EAT suffers greater oxidative stress than SAT in patients with cardiovascular diseases and exhibits associated proteomic differences that suggest the possibility of its association with myocardial stress in these patients. epicardial adipose tisssue OBESITY IS ONE OF THE MAIN causes of metabolic diseases. It is associated with a chronic inflammatory response and cardiovascular diseases (34). However, risk depends significantly on the distribution of adipose tissue in the body; for example, metabolic risk factors were more associated with omental adipose tissue (OAT) than with subcutaneous adipose tissue (SAT) among 3,001 participants in the Framingham Heart Study (6). These differences are plausibly due to different adipose tissues differing in adipokine expression and the production of inflammatory reactive oxygen species (ROS), which are known to be associated with cardiovascular disease (8); OAT is associated with more circulating substances related to inflammation and oxidative stress than SAT is (24).Epicardial adipose tissue (EAT) is a visceral fat pad adjacent to coronary arteries and the myocardium (11). The amount of EAT is related to left ventricle mass (13) and to the amount of intra-abdominal visceral fat, and visceral adiposity can in fact be evaluated by means of its echocardiographic measurement (10). Like other adipose tissues, EAT is now recognized as an endocrine organ, and its proximity to myocardium and coronary arteries suggests the possibility of paracrine action on these structures. In fact, in patients with coronary ...

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confidence: 99%

Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress

Salgado-Somoza¹,

Teijeira-Fernández

Fernández

et al. 2010

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

141

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“…Epicardial adipose tissue (EAT) is the visceral adipose tissue located in the atrioventricular and interventricular grooves, adjacent to adventitia of coronary arteries without fascia structures. This tissue is a source of pro-inflammatory cytokines (6)(7)(8) and contains low levels of adiponectin, an anti-inflammatory adipokine, in patients with CAD (9,10). Moreover, adiponectin expression in EAT decreases in patients with hypertension (11); although its levels are not modified in diabetes (12).…”

Section: Introductionmentioning

confidence: 99%

Receptor for advanced glycation end-products expression in subcutaneous adipose tissue is related to coronary artery disease

Rodiño-Janeiro

Salgado-Somoza²,

Teijeira-Fernández³

et al. 2011

European Journal of Endocrinology

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Objective: Obesity, a risk factor for coronary artery disease (CAD), is associated with inflammation and reactive oxygen species (ROS) production, while advanced glycation end-products, through their receptor (AGER or RAGE), play an important role on these processes. The aim of this study was to analyze the expression levels of RAGE, NADPH oxidase subunits, and catalase in adipose tissue in relation with CAD. Design and methods: Patients undergoing heart surgery were included in two groups: with and without CAD. Epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) biopsies were analyzed for gene expression by RT-quantitative PCR, immunohistochemistry, or western blot. Results: RAGE mRNA and protein expression in SAT from patients with CAD was lower than in patients without CAD. However, there was no change in EAT from patients with or without CAD. P22-PHOX and RAGE gene expression were higher in EAT than in SAT, whereas catalase mRNA levels were lower. NADPH oxidase subunits and catalase mRNA expression were not influenced by CAD. Whereas NADPH oxidase-dependent oxidative response of SAT and EAT to lipid circulating levels could be different; glycemic levels were not related with the analyzed genes expression. Conclusions: This study demonstrates that RAGE expression in SAT, but not in EAT, is down-regulated in patients with CAD with respect to those without CAD. Although changes were not observed for NADPH oxidase subunits or catalase expression between CAD and non-CAD patients, a possible relationship between ROS production and RAGE expression in adipose tissues cannot be ruled out.

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“…A previous study has reported that level of APN released from EAT local expression were lower in patients with hypertension (34) as well as lower levels of the hormone were related to the promotion of coronary artery disease (35). Another study has suggested that local expression of APN has local effect on myo- cardium and coronary artery rather than systemic effects (36).…”

Section: Discussionmentioning

confidence: 99%

The evaluation of relationship between adiponectin levels and epicardial adipose tissue thickness with low cardiac risk in Gilbert`s syndrome: an observational study

Cüre¹,

Çiçek²,

Cüre³

et al. 2013

Anadolu Kardiyol Derg

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Objective: Atherosclerotic heart diseases are less frequently seen in patients with Gilbert's syndrome (GS). We aimed to investigate whether serum adiponectin (APN) and epicardial adipose tissue (EAT) thickness have an effect beside the antioxidant effect of bilirubin in lowering the incidence of the atherosclerotic process. Methods: Sixty-eight patients diagnosed with GS (39 females and 29 males) who had applied at the internal medicine clinic of the hospital were included in this cross-sectional, observational study. The control group included 63 healthy people (39 females and 24 males). EAT thickness was measured by echocardiography. The serum APN levels were also checked. Statistical analysis was performed by using independent sample t-test, Pearson correlation and linear regression analyses. Results: The mean age of the GS group was 28±9 years, and the average EAT thickness was found to be 2.5±0.1 mm. The mean age of the control group was 26±6 years, and the average EAT thickness was found to be 4.2±0.5 mm. When comparing the two groups, the EAT thickness of the GS group was found to be significantly lower (p<0.001) than that of the control group. In the GS group the APN was 14.9±4.2 mg/L, and in the control group the APN was 12.6±4.5 mg/L (p<0.022). We found that total bilirubin (β=-1,607, p<0,001) and indirect bilirubin (β=1,086, p<0,001) have an independent association with decreased EAT thickness. Conclusion: EAT thickness is associated with coronary atherosclerosis. Low EAT thickness may be related with low release of proinflammatory cytokine. High levels of APN may be related high anti-inflammatory effect. Therefore, low EAT thickness and high levels of APN may demonstrate protective effect on atherosclerotic heart diseases in GS patients. (

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Extension of coronary artery disease is associated with increased IL-6 and decreased adiponectin gene expression in epicardial adipose tissue

Cited by 116 publications

References 29 publications

Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress

Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress

Receptor for advanced glycation end-products expression in subcutaneous adipose tissue is related to coronary artery disease

The evaluation of relationship between adiponectin levels and epicardial adipose tissue thickness with low cardiac risk in Gilbert`s syndrome: an observational study

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