Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene.

Croizier, G.; Croizier, L.; Argaud, Olivier; Poudevigne, Didier

doi:10.1073/pnas.91.1.48

Cited by 118 publications

(76 citation statements)

References 28 publications

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“…The p143 gene of AcMNPV has helicase activity (McDougal & Guarino, 2001) and has been implicated as a host-range factor (Croizier et al, 1994;Kamita & Maeda, 1997). For example, although AcMNPV and BmNPV share high DNA sequence similarity, AcMNPV could not replicate in B. mori cells and BmNPV could not replicate in Sf21 cells.…”

Section: Discussionmentioning

confidence: 99%

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Slow cell infection, inefficient primary infection and inability to replicate in the fat body determine the host range of Thysanoplusia orichalcea nucleopolyhedrovirus

Wang

Salem

Lynn

et al. 2008

Journal of General Virology

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Thysanoplusia orichacea multicapsid nucleopolyhedrovirus (ThorMNPV) carrying an enhanced green fluorescent protein (EGFP) gene expression cassette (vThGFP) was used to study host-range mechanisms. Infection kinetics showed that vThGFP replication in Sf21 cells was too slow to suppress cell growth. Wide-host-range Autographa californica MNPV (AcMNPV) could speed up vThGFP infection and enhance the vThGFP infection rate in Sf21 cells. The enhancement was not due to recombination, as no recombinant virus was isolated from coinfection by plaque assay. No improvement of vThGFP infection in Sf21 was found by AcMNPV cosmid transactivation assay. However, culture medium from Sf21 cells infected with AcMNPV did enhance vThGFP replication in Sf21. Third-instar larvae of Spodoptera frugiperda, S. exigua and Helicoverpa zea were not killed by feeding with vThGFP polyhedra but were killed by intrahaemocoelic injection using budded viruses (BVs). This suggested that insufficient BVs were generated during the primary infection in the midgut. vThGFP infected haemocytes, tracheae and Malpighian tubules but not fat bodies of larvae of S. frugiperda, S. exigua and H. zea. Third-instar S. frugiperda larvae co-infected by injection with vThGFP and vAcDsRed2, an AcMNPV expressing a red fluorescent protein gene, showed EGFP expression in the fat body. This result suggests that vAcDsRed2 could help vThGFP to replicate in the fat body or trans-activate EGFP expression in the fat body. All these results suggested that slow cell infection, insufficient primary infection and inability to replicate in the fat body control the host range of ThorMNPV.

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Section: Discussionmentioning

confidence: 99%

“…For example, although AcMNPV and BmNPV share high DNA sequence similarity, AcMNPV could not replicate in B. mori cells and BmNPV could not replicate in Sf21 cells. The inability of AcMNPV to replicate in B. mori cells is due to the p143 gene of AcMNPV (Croizier et al, 1994;Kamita & Maeda, 1997).…”

Section: Discussionmentioning

confidence: 99%

Slow cell infection, inefficient primary infection and inability to replicate in the fat body determine the host range of Thysanoplusia orichalcea nucleopolyhedrovirus

Wang

Salem

Lynn

et al. 2008

Journal of General Virology

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“…Recent studies have identified several viral genes that are involved in host range determination of Autographa californica multinucleocapsid NPV (AcMNPV) in insect cell systems (6,7,18,21,22,24,31). One of these genes, host range factor 1 (hrf-1) from the gypsy moth NPV, Lymantria dispar MNPV (LdMNPV), was identified as a factor that promoted AcMNPV replication in nonpermissive cell line Ld652Y (12), derived from L. dispar.…”

mentioning

confidence: 99%

Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells Derived from L. dispar

Ishikawa

Ikeda

Alves

et al. 2004

J Virol

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Host range factor 1 (HRF-1) of Lymantria dispar multinucleocapsid nucleopolyhedrovirus promotes Autographa californica MNPV replication in nonpermissive Ld652Y cells derived from L. dispar. Here we demonstrate that restricted Hyphantria cunea NPV replication in Ld652Y cells was not due to apoptosis but was likely due to global protein synthesis arrest that could be restored by HRF-1. Our data also showed that HRF-1 promoted the production of progeny virions for two other baculoviruses, Bombyx mori NPV and Spodoptera exigua MNPV, whose replication in Ld652Y cells is limited to replication of viral DNA without successful production of infectious progeny virions. Thus, HRF-1 is an essential viral factor required for productive infection of NPVs in Ld652Y cells.Nucleopolyhedroviruses (NPVs) belong to the family Baculoviridae and possess a double-stranded, circular DNA genome of 80 to 180 kbp within a large rod-shaped capsid (4). These viruses are insect-specific viruses reported from more than 520 insect species (1). NPVs generally exhibit a narrow host range property (1). Lethal infection is commonly restricted to insect species from which the NPVs are originally isolated and to closely related insect species. Analyses with cell cultures have revealed that while budded virions (BV) of NPVs are capable of entering a phylogenetically broad range of insect cells, NPV replication is often restricted at a step after viral entry that differs depending on the specific combinations of NPVs and insect cell lines (20,27,29,33,34).The molecular mechanisms underlying the host specificity of NPVs are not clear. Recent studies have identified several viral genes that are involved in host range determination of Autographa californica multinucleocapsid NPV (AcMNPV) in insect cell systems (6,7,18,21,22,24,31). One of these genes, host range factor 1 (hrf-1) from the gypsy moth NPV, Lymantria dispar MNPV (LdMNPV), was identified as a factor that promoted AcMNPV replication in nonpermissive cell line Ld652Y (12), derived from L. dispar. AcMNPV infection of Ld652Y cells results in global protein synthesis shutoff and yields no progeny virions (8,13,14,23,32). The recombinant AcMNPV that possesses hrf-1 restores viral protein synthesis and replicates successfully in Ld652Y cells and L. dispar larvae (5, 7, 31). Thus, HRF-1 protein precludes global protein synthesis shutoff and promotes production of progeny virions in AcMNPV-infected Ld652Y cells. Analyses of whole-genome sequences from several NPVs (2,3,11,15,16,19,28) revealed that hrf-1 was specifically located on the genome of LdMNPV and Orgyia pseudotsugata MNPV that could replicate in Ld652Y cells. In this study, we demonstrate that HRF-1 is an essential factor required for NPVs to replicate successfully in Ld652Y cells.HycuNPV replication is restricted in Ld652Y cells by a mechanism other than apoptosis. It was previously shown that infection of Ld652Y cells with Hyphantria cunea NPV (HycuNPV) resulted in induction of severe cellular apoptosis in which no progeny virions were pr...

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“…Though DNA replication seemingly occurred as in the permissive Sf-21 cells, the defect in AcMNPV-BmN cells was shown to be caused by differences in the DNA helicase gene (p143) (Maeda et al, 1993). A few amino acid changes in AcMNV P143 were sufficient to overcome the defect in B. mori cells and larvae (Argaud et al, 1998;Croizier et al, 1994). The cytotoxicity and block in AcMNPV infection of B. mori cells are suggested to stem from aberrant DNA replication (reviewed in Thiem & Cheng, 2009;Rohrmann, 2011).…”

Section: Late Phase -Dna Replicationmentioning

confidence: 99%

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

Lapointe¹,

Thumbi²,

Lucarotti³

2012

Integrated Pest Management and Pest Control - Current and Future Tactics

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Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene.

Cited by 118 publications

References 28 publications

Slow cell infection, inefficient primary infection and inability to replicate in the fat body determine the host range of Thysanoplusia orichalcea nucleopolyhedrovirus

Slow cell infection, inefficient primary infection and inability to replicate in the fat body determine the host range of Thysanoplusia orichalcea nucleopolyhedrovirus

Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells Derived from L. dispar

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

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