Extending the scope of neuropeptidomics in the mammalian brain

Zhang, Xiaozhe; Petruzziello, Filomena; Rainer, Gregor

doi:10.1016/j.euprot.2014.04.004

Cited by 7 publications

(8 citation statements)

References 46 publications

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“…Although more than one hundred neuropeptides have been discovered, the role of only a few neuropeptides in nicotine withdrawal have been investigated (Bruijnzeel 2012; Zhang et al 2014). In our laboratory, we investigated the effects of NPY on somatic and affective nicotine withdrawal signs (Rylkova et al 2008).…”

Section: Dysphoria and Nicotine Withdrawalmentioning

confidence: 99%

Neuropeptide systems and new treatments for nicotine addiction

Bruijnzeel

2016

Psychopharmacology

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RATIONALE The mildly euphoric and cognitive enhancing effects of nicotine play a role in the initiation of smoking, while dysphoria and anxiety associated with smoking cessation contribute to relapse. After the acute withdrawal phase, smoking cues, a few cigarettes (i.e., lapse), and stressors can cause relapse. Human and animal studies have shown that neuropeptides play a critical role in nicotine addiction. OBJECTIVES The goal of this paper is to describe the role of neuropeptide systems in the initiation of nicotine intake, nicotine withdrawal, and the reinstatement of extinguished nicotine seeking. RESULTS The reviewed studies indicate that several drugs that target neuropeptide systems diminish the rewarding effects of nicotine by preventing the activation of dopaminergic systems. Other peptide-based drugs diminish the hyperactivity of brain stress systems and diminish withdrawal-associated symptom severity. Blockade of hypocretin-1 and nociceptin receptors and stimulation of galanin and neurotensin receptors diminishes the rewarding effects of nicotine. Both corticotropin-releasing factor type 1 and kappa-opioid receptor antagonists diminish dysphoria and anxiety-like behavior associated with nicotine withdrawal and inhibit stress-induced reinstatement of nicotine seeking. Furthermore, blockade of vasopressin 1b receptors diminishes dysphoria during nicotine withdrawal and melanocortin 4 receptor blockade prevents stress-induced reinstatement of nicotine seeking. The role of neuropeptide systems in nicotine-primed and cue-induced reinstatement is largely unexplored, but there is evidence for a role of hypocretin-1 receptors in cue-induced reinstatement of nicotine seeking. CONCLUSION Drugs that target neuropeptide systems might decrease the euphoric effects of smoking and improve relapse rates by diminishing withdrawal symptoms and improving stress resilience.

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Section: Dysphoria and Nicotine Withdrawalmentioning

confidence: 99%

Neuropeptide systems and new treatments for nicotine addiction

Bruijnzeel

2016

Psychopharmacology

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“…Previous studies have shown that independent of fragmentation technique, the peptide spectral matches (PSMs) are largely determined by the speed of fragmentation and abundance of peptides, 26 which suggests that most of the low-abundance peptides are subject to a low chance of fragmentation, 27 consequently leading to tandem MS spectra with poor quality and an unsatisfactory identification rate. 28 Workflow for Mining the MS Data. To efficiently detect disulfide bonded peptides, we developed this data mining strategy to recognize disulfide bonded peptide candidates from brain extracts (Scheme 1).…”

Section: Inspection Of Disulfide Bonded Peptides Usingmentioning

confidence: 99%

High-Efficiency Recognition and Identification of Disulfide Bonded Peptides in Rat Neuropeptidome Using Targeted Electron Transfer Dissociation Tandem Mass Spectrometry

Khani

et al. 2015

Anal. Chem.

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ABSTRACT:The main goal of the present study is to develop a method to recognize and identify endogenous intrachain disulfide bonded peptide, which are rarely sequenced in current peptidomics studies. In order to achieve highly efficient detection of these peptides in a neuropeptidome analysis, we alkylated the peptides, mined the raw mass spectrometry data, and then recognized the candidates of untreated disulfide bonded peptides from unalkylated peptide extracts. After removing more than 90% features, targeted electron transfer dissociation fragmentation was performed for detecting and fragmenting disulfide bonded peptides, and even most of them were present in low abundance in the original sample. Diverse endogenous disulfide bonded peptides were then detected and sequenced, opening up new perspectives for comprehensively understanding the response of a neuropeptidome.

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“…As neuropeptides are a highly diverse class of signaling molecules in the brain and other peripheral organs, their structures, functions and localization are of great interest and relevance (Hughes and Woodruff, 1992). The extent of their implied roles in normal biological processes has been a point of focus in studies over the years (Hook and Bandeira, 2015;Zhang et al, 2014). Abnormalities in their expression can contribute to various neurological diseases by altering the function of specific neurons, and so understanding the mechanisms of neuropeptide signaling can help researchers to better understand these diseases and develop more focused and effective treatments (Beal and Martin, 2016).…”

Section: Introductionmentioning

confidence: 99%

New techniques, applications and perspectives in neuropeptide research

DeLaney

Buchberger

Atkinson

et al. 2018

Journal of Experimental Biology

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Neuropeptides are one of the most diverse classes of signaling molecules and have attracted great interest over the years owing to their roles in regulation of a wide range of physiological processes. However, there are unique challenges associated with neuropeptide studies stemming from the highly variable molecular sizes of the peptides, low concentrations, high degree of structural diversity and large number of isoforms. As a result, much effort has been focused on developing new techniques for studying neuropeptides, as well as novel applications directed towards learning more about these endogenous peptides. The areas of importance for neuropeptide studies include structure, localization within tissues, interaction with their receptors, including ion channels, and physiological function. Here, we discuss these aspects and the associated techniques, focusing on technologies that have demonstrated potential in advancing the field in recent years. Most identification and structural information has been gained by mass spectrometry, either alone or with confirmations from other techniques, such as nuclear magnetic resonance spectroscopy and other spectroscopic tools. While mass spectrometry and bioinformatic tools have proven to be the most powerful for large-scale analyses, they still rely heavily on complementary methods for confirmation. Localization within tissues, for example, can be probed by mass spectrometry imaging, immunohistochemistry and radioimmunoassays. Functional information has been gained primarily from behavioral studies coupled with tissue-specific assays, electrophysiology, mass spectrometry and optogenetic tools. Concerning the receptors for neuropeptides, the discovery of ion channels that are directly gated by neuropeptides opens up the possibility of developing a new generation of tools for neuroscience, which could be used to monitor neuropeptide release or to specifically change the membrane potential of neurons. It is expected that future neuropeptide research will involve the integration of complementary bioanalytical technologies and functional assays.

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Extending the scope of neuropeptidomics in the mammalian brain

Cited by 7 publications

References 46 publications

Neuropeptide systems and new treatments for nicotine addiction

Neuropeptide systems and new treatments for nicotine addiction

High-Efficiency Recognition and Identification of Disulfide Bonded Peptides in Rat Neuropeptidome Using Targeted Electron Transfer Dissociation Tandem Mass Spectrometry

New techniques, applications and perspectives in neuropeptide research

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