Extending the range of distances accessible by 19F electron-nuclear double resonance in proteins using high-spin Gd(III) labels
Alexey Bogdanov,
Veronica Frydman,
Manas Seal
et al.
Abstract:Fluorine electron-nuclear double resonance (19F ENDOR) has recently emerged as a valuable tool in structural biology for distance determination between F atoms and a paramagnetic center, either intrinsic or conjugated to a biomolecule via spin labeling, yielding distances beyond those accessible by double electron-electron resonance (DEER). To further extend the accessible distance range we exploit the high-spin properties of Gd(III) and focus on transitions other than the central transition (|–1/2> <-&g… Show more
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