Extending the limits of Bacillus for novel biotechnological applications

Kumar, Prasun; Patel, Sanjay; Lee, Jung-Kul; Kalia, Vipin Chandra

doi:10.1016/j.biotechadv.2013.08.007

Cited by 211 publications

(126 citation statements)

References 212 publications

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“…In most cases, scl-PHA are produced, which include PHB, P(3HB:3HV), P(3HB:4HB), P(3HB:3HP) and P(3HB:3HV:4HB) [3,4,16,17]. PHAs having mcl-HAs include P(3HB:3HB:3HHx), P(3HHx:3HO:3HD), and P(3HHx:3HO:3HD:3HDD).…”

Section: Factors Affecting Pha Characteristics Monomeric Composition mentioning

confidence: 99%

“…PHA polymers, which are less commonly observed, include other non-PHB homopolymers of 4HB, 3HV, 3H5PV, 3HHx, 3HHp, 3HO and 3HN. The M w of PHA covers a wide range from 0.5 9 10 5 to 35 9 10 5 Da and their polydispersity index (PDI) varies between 1.1 and 6.0 [4]. PDI is the index for the distribution of molecules within a polymer sample, which is measured as ratio of the weight (M w ) versus number (M n ) average molecular weight.…”

Section: Factors Affecting Pha Characteristics Monomeric Composition mentioning

confidence: 99%

“…3HHx. PHAs varying from PHB, P(3HB:3HV), P(3HB:3HHx), P(3HB:4HB:3HHx) to P(3HB:6HHx:3HHx) depending upon the substrate [3,4,30,32,33]. Bacillus provides a robust option as potential PHA producer under more stringent conditions [3,4].…”

Section: Bacterial Pha Synthasesmentioning

confidence: 99%

“…PHAs with varying composition can be produced by microbes by fermenting an array of substrates [1,2]. High cost of production and difficulties in processing the homopolymers of PHAs makes them less favourable for large scale usage [2][3][4][5]. Efforts to improve the monomer composition and their molecular weight (M w ) have resulted in polymers, which are more suitable for downstream processing.…”

Section: Introductionmentioning

confidence: 99%

“…The major challenges to make PHAs economically viable, is to make them easier to process and handle. Manipulating the genetic makeup of bacteria appears to be the most obvious choice for achieving these targets [4,5,14]. Interestingly, this can be also achieved by adopting strategies such as selection of bacteria, suitable substrates, appropriate feeding regime, and variations in physiological conditions [15].…”

Section: Introductionmentioning

confidence: 99%

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Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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Section: Factors Affecting Pha Characteristics Monomeric Composition mentioning

confidence: 99%

Section: Factors Affecting Pha Characteristics Monomeric Composition mentioning

confidence: 99%