Extending the Host Range of Fusarium Poae Virus 1 from Fusarium poae to other Fusarium Species in the Field

Song, Xiu-Shi; Sun, Yuqing; Gao, Jing; Gu, Kai-Xin; Hou, Yiping; Wang, Jianxin; Zhou, Mingguo

doi:10.3390/v14102246

Cited by 3 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first report of interspecific transmission of dsRNA was the transmission of Sclerotinia sclerotiorum virus 1 (Ssv1) from Sclerotinia sclerotiorum to S. minor through hyphal anastomosis [ 61 ]. Fusarium poae virus 1-Fa (FpV1-Fa) was also reported to be transferred from F. asiaticum to F. poae and F. tricinctum through hyphal anastomosis [ 62 ]. FgV1-DK21 virus was transmitted horizontally from F. boothii to F. graminearum , F. asiaticum , F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

View full text Add to dashboard Cite

A novel virus with a double-stranded RNA (dsRNA) genome was isolated from Fusarium avenaceum strain GS-WW-224, the causal agent of potato dry rot. The virus has been designated as Fusarium avenaceum alternavirus 1 (FaAV1). Its genome consists of two dsRNA segments, 3538 bp (dsRNA1) and 2477 bp (dsRNA2) in length, encoding RNA-dependent RNA polymerase (RdRp) and a hypothetical protein (HP), respectively. The virions of FaAV1 are isometric spherical and approximately 30 nm in diameter. Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FaAV1 appears to be a new member of the proposed family Alternaviridae. No significant differences in colony morphology and spore production were observed between strains GS-WW-224 and GS-WW-224-VF, the latter strain being one in which FaAV1 was eliminated from strain GS-WW-224. Notably, however, the dry weight of mycelial biomass of GS-WW-224 was higher than that of mycelial biomass of GS-WW-224-VF. The depth and the width of lesions on potato tubers caused by GS-WW-224 were significantly greater, relative to GS-WW-224-VF, suggesting that FaAV1 confers hypervirulence to its host, F. avenaceum. Moreover, FaAV1 was successfully transmitted horizontally from GS-WW-224 to ten other species of Fusarium, and purified virions of FaAV1 were capable of transfecting wounded hyphae of the ten species of Fusarium. This is the first report of an alternavirus infecting F. avenaceum and conferring hypervirulence.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

“…For instance, a RNA virus from Sclerotinia homoeocarpa shares 92.4% nucleotide and 95.1% amino acid sequence identities with the Ophiostoma novo-ulmi mitovirus 3a-Ld from Ophiostoma novo-ulmi ( Deng et al, 2003 ). Increasing evidence suggests that mycoviruses may have a broader host range in nature and could potentially spread between different hosts through unknown mechanisms ( Khalifa and MacDiarmid, 2019 ; Song et al, 2022 ). Additionally, some studies indicate that under laboratory conditions, mycoviruses can achieve cross-species transmission in certain fungi ( Melzer et al, 2002 ; Ning et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Cross-species transmission of a novel bisegmented orfanplasmovirus in the phytopathogenic fungus Exserohilum rostratum

Jia,

Nan,

Song

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Mycoviruses have been found in various fungal species across different taxonomic groups, while no viruses have been reported yet in the fungus Exserohilum rostratum. In this study, a novel orfanplasmovirus, namely Exserohilum rostratum orfanplasmovirus 1 (ErOrfV1), was identified in the Exserohilum rostratum strain JZ1 from maize leaf. The complete genome of ErOrfV1 consists of two positive single-stranded RNA segments, encoding an RNA-dependent RNA polymerase and a hypothetical protein with unknown function, respectively. Phylogenetic analysis revealed that ErOrfV1 clusters with other orfanplasmoviruses, forming a distinct phyletic clade. A new family, Orfanplasmoviridae, is proposed to encompass this newly discovered ErOrfV1 and its associated orfanplasmoviruses. ErOrfV1 exhibits effective vertical transmission through conidia, as evidenced by its 100% presence in over 200 single conidium isolates. Moreover, it can be horizontally transmitted to Exserohilum turcicum. Additionally, the infection of ErOrfV1 is cryptic in E. turcicum because there were no significant differences in mycelial growth rate and colony morphology between ErOrfV1-infected and ErOrfV1-free strains. This study represents the inaugural report of a mycovirus in E. rostratum, as well as the first documentation of the biological and transmission characteristics of orfanplasmovirus. These discoveries significantly contribute to our understanding of orfanplasmovirus.

show abstract

“…Momordicae , recipient strain, which causes Fusarium wilt of bitter gourd), and thus conferred hypovirulence on its recipient strain [ 11 ]. In particular, a strain of Fusarium poae virus 1 (FpV1-Fa) identified from F. asiaticum could transfer from its host fungus, F. asiaticum , to two other species of Fusarium , F. poae and F. tricinctum ; consequently, the virulence of the FpV1-Fa recipient strain ( F. poae or F. tricinctum ) was reduced [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Strain of Fusarium oxysporum Alternavirus 1 Isolated from Fusarium oxysporum f. sp. melonis Strain T-BJ17 Confers Hypovirulence and Increases the Sensitivity of Its Host Fungus to Difenoconazole and Pydiflumetofen

Hua,

Zhang,

Liu

et al. 2024

Viruses

View full text Add to dashboard Cite

In the current study, a novel strain of Fusarium oxysporum alternavirus 1 (FoAV1) was identified from the Fusarium oxysporum f. sp. melonis (FOM) strain T-BJ17 and was designated as Fusarium oxysporum alternavirus 1-FOM (FoAV1-FOM). Its genome consists of four dsRNA segments of 3515 bp (dsRNA1), 2663 bp (dsRNA2), 2368 bp (dsRNA3), and 1776 bp (dsRNA4) in length. Open reading frame 1 (ORF1) in dsRNA1 was found to encode a putative RNA-dependent RNA polymerase (RdRp), whose amino acid sequence was 99.02% identical to that of its counterpart in FoAV1; while ORF2 in dsRNA2, ORF3 in dsRNA3, and ORF4 in dsRNA4 were all found to encode hypothetical proteins. Strain T-BJ17-VF, which was verified to FoAV1-FOM-free, was obtained using single-hyphal-tip culture combined with high-temperature treatment to eliminate FoAV1-FOM from strain T-BJ17. The colony growth rate, ability to produce spores, and virulence of strain T-BJ17 were significantly lower than those of T-BJ17-VF, while the dry weight of the mycelial biomass and the sensitivity to difenoconazole and pydiflumetofen of strain T-BJ17 were greater than those of T-BJ17-VF. FoAV1-FOM was capable of 100% vertical transmission via spores. To our knowledge, this is the first time that an alternavirus has infected FOM, and this is the first report of hypovirulence and increased sensitivity to difenoconazole and pydiflumetofen induced by FoAV1-FOM infection in FOM.

show abstract

Extending the Host Range of Fusarium Poae Virus 1 from Fusarium poae to other Fusarium Species in the Field

Cited by 3 publications

References 50 publications

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Cross-species transmission of a novel bisegmented orfanplasmovirus in the phytopathogenic fungus Exserohilum rostratum

A Novel Strain of Fusarium oxysporum Alternavirus 1 Isolated from Fusarium oxysporum f. sp. melonis Strain T-BJ17 Confers Hypovirulence and Increases the Sensitivity of Its Host Fungus to Difenoconazole and Pydiflumetofen

Contact Info

Product

Resources

About