Extended Spectrum β-Lactamase-Producing Strains of Escherichia coli in Hospitalized Children in Isfahan, Iran

Abstract

Background: Beta-lactam antibiotics such as long-spectrum cephalosporins and carbapenems are the empirical treatment against urinary tract infections (UTIs). The common mechanism of resistance to B-lactamas in Escherichia coli is producing of ESBLs. Objectives: The aims of this study was evaluation of the susceptibility of the ESBL-producing E. coli strains to ordinarily used antibiotics and to detect the presence of the four common ESBL genes: blaTEM, blaOXA, blaCTX-M and blaSHV using multiplex PCR method. Materials and Methods: In a retrospective cross sectional study conducted between 2012 and 2013, out of145 strains of E. coli were isolated from hospitalized children with UTIs. Susceptibility test was performed by disc diffusion method. All isolates were also tested for ESBL by the standard CLSI double disk diffusion method, using cefotaxime/ clavulanic acid and ceftazidime/clavulanic acid disks. Multiplex PCR was performed for detect of blaCTX-MIV, blaOXA, blaTEM and blaSHV genes. Results: From 145 strains of E. coli, 51(35.2%) and 61(42.1%) isolates were resistance to ceftazidime and cefotaxime, respectively. ESBL production was noted in 59 (40.7%) strains. The amplification of β-lactamase genes showed the presence of blaCTX-M like genes in 22 (37.9%) strains, blaTEM in 42 (72.4%), blaSHV in 7 (11.9%) and blaOXA in 23 (39%) of the total 58 strains of E. coli. Conclusions: Our data showed that, the high prevalence of beta-lactamase genes among isolates, and it. Our findings may provide useful insights in replace of the appropriate antibiotics and it may also prevents of ESBLs, mediated resistance problem.

The evaluation of phenotyping and molecular resistance to antibiotics in Proteus species isolated from urinary tract infections in Ilam city

“…Continuous variables were described as mean ± standard deviation (SD) and compared with standard student t test, or and calculates median range. All the tests were two-tailed and P < 0.05 was considered statistically significant (9).…”

Whole Genome Characterization of the High-Risk Clone ST383 Klebsiella pneumoniae with a Simultaneous Carriage of blaCTX-M-14 on IncL/M Plasmid and blaCTX-M-15 on Convergent IncHI1B/IncFIB Plasmid from Egypt

“…Colony PCR was performed to detect the genes coding for the CTX-M enzymes of Group IV among the collected isolates [20]. The following primers, obtained from Willowfort, United Kingdom, were used: bla CTX-M-IV -forward (5 -GACAAAGAGAGTGCAACGG-ATG-3 ) and bla CTX-M-IV -reverse (5 -TCAGTGCGATCCAGACGAAA-3 ) [21]. The obtained PCR products were separated by gel electrophoresis in the presence of 100 bp DNA ladder (GeneDireX ® , Miaoli, Taiwan) used as a marker.…”

ANTIMICROBIAL SUSCEPTIBILITY AND DETECTION METHODS FOR THE EXTENDED‑SPECTRUM Β-Lactamases PRODUCING ENTEROBACTERIACEAE FROM CLINICAL SAMPLES

“…ESBLs are often associated with plasmids harboring resistance genes to other antimicrobial classes, resulting in multidrug-resistant isolates thereby facilitating their transfer to different hosts [12,13]. The first ESBLs have evolved from native β-lactamases TEM and SHV by a genetic mutation.…”