Extended-spectrum β-lactamase-producing Salmonella enterica serovar Infantis from humans in Italy

Dionisi, Anna Maria; Owczarek, Slawomir; Benedetti, Ildo; Luzzi, Ida; García-Fernández, Aurora

doi:10.1016/j.ijantimicag.2016.06.025

Cited by 6 publications

(5 citation statements)

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“…Dionisi et al observed that S . Infantis ESBL-producing strains isolated from humans in Italy belonged to profiles XbaI0125, XbaI0126, XbaI0129 and XbaI2116, different from the one we found [ 31 ]. However, these profiles had the same resistance core detected by us in the profile XbaI.2621, except the resistance to cefepime.…”

Section: Discussioncontrasting

confidence: 84%

“…The remaining strains belonged to clonally related groups, having a genetic homology from 81% to 97% with the XbaI.0126 profile, including the XbaI.0125 cluster. The strains belonging to these profiles, described only in humans by Dionisi et al [31], were isolated from different matrices collected along the broiler food chain independently on the year and on the region. However, it should be further underlined that in the previous investigations on Italian S. Infantis strains the ECDC nomenclature has not been used for the PFGE analysis [4,12].…”

Section: Discussionmentioning

confidence: 99%

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Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production

et al. 2020

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This work aimed to evaluate the antimicrobial susceptibility of 87 Salmonella Infantis strains isolated in Italy from 2016 to 2019 along the food chain of broiler meat production and in humans and to determine the genetic profiles of the strains in order to establish a possible correlation with the antimicrobial pattern. All isolates were tested by the disk diffusion method to evaluate antimicrobial susceptibility toward sixteen antimicrobials, and the broth microdilution method was used to confirm extended spectrum β-lactamase (ESBL) production. PCR and pulsed field gel electrophoresis (PFGE) were applied to characterize ESBL-encoding and AmpC β-lactamase genes and to analyze the S. Infantis strains genetic profiles respectively. S. Infantis isolates showed high prevalence of resistance, in particular toward nalidixic acid (97.7%), tetracycline (96.5%), sulphamethoxazole/trimethoprim (91%) and cefepime (72.4%). The 80.5% of isolates were ESBL, cefotaxime-resistant, carrying the blaCTX-M1 gene. The most prevalent PFGE profile was XbaI.0126 (35.6%). The remaining strains had a genetic homology from 81% to 97% with the XbaI.0126 profile. The strains belonging to these profiles were isolated from different matrices collected along the broiler food chain independently on the year and from the region and there was no correlation between the PFGE profiles and resistance patterns. We found two ESBL-producing S. Infantis strains with the same XbaI.2621 profile isolated from humans and from poultry feces, not yet reported in Italy. Our findings confirmed the diffusion of ESBL-multi drug resistant (MDR) S. Infantis along the broiler food chain and in humans and underlined the importance of continuous monitoring to control and to reduce the prevalence of this bacterium, applying a global One Health approach.

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Section: Discussioncontrasting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production

et al. 2020

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“…The real incompatibility group of some pESI-like plasmids is not clearly definable, because the replication origin of these plasmids is not conventional. It results from the substitution of the IncI1 oriV by an IncP-1alpha oriV (Aviv et al, 2014;Dionisi et al, 2016). This could be one reason for the controversial findings described above regarding incompatibility of pSI54/04, basically confirming findings of Bogomazova et al (2020) in relation to comparative analysis of a selected set of pESI-like plasmid-bearing strains on the basis of whole-genome sequencing.…”

Section: Discussionsupporting

confidence: 70%

Comparative genomics of emerging lineages and mobile resistomes of contemporary broiler strains ofSalmonellaInfantis andE. coli

Szmolka

Wami

Dobrindt

2021

Preprint

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IntroductionCommensal and pathogenic strains of MDR Escherichia coli and non-typhoid strains of Salmonella represent a growing foodborne threat from foods of poultry origin. MDR strains of S. Infantis and E. coli are frequently isolated from broiler chicks and the simultaneous presence of these two enteric bacterial species would potentially allow the exchange of mobile resistance determinants.ObjectivesIn order to understand possible genomic relations and to obtain a first insight into the potential interplay of resistance genes between enteric bacteria, we compared genomic diversity and mobile resistomes of S. Infantis and E. coli from broiler sources.ResultsThe cgMLST analysis of 56 S. Infantis and 90 E. coli contemporary strains revealed a high genomic heterogeneity of broiler E. coli. It also allowed the first insight into the genomic diversity of the MDR clone B2 of S. Infantis, which is endemic in Hungary. We also identified new MDR lineages for S. Infantis (ST7081 and ST7082) and for E. coli (ST8702 and ST10088). Comparative analysis of antibiotic resistance genes and plasmid types revealed a relatively narrow interface between the mobile resistomes of E. coli and S. Infantis. The mobile resistance genes tet(A), aadA1 and sul1 were identified at an overall high prevalence in both species. This gene association is characteristic to the plasmid pSI54/04 of the epidemic clone B2 of S. Infantis. Simultaneous presence of these genes and of IncI and IncX plasmids in cohabitant caecal strains of E. coli and S. Infantis suggests an important role of these plasmids in a possible interplay of resistance genes between S. Infantis and E. coli in broilers.ConclusionsThis is the first comparative genomic analysis of contemporary broiler strains of S. Infantis and E. coli. The diversity of mobile resistomes suggests that commensal E. coli could be potential reservoirs of resistance for S. Infantis, but so far only a few plasmid types and mobile resistance genes could be considered as potentially exchangeable between these two species. Among these, IncI plasmids could make the greatest contribution to the microevolution and genetic interaction between E. coli and S. Infantis.

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“…The real incompatibility group of some pESIlike plasmids is not clearly definable, because the replication origin of these plasmids is not conventional. It results from the substitution of the IncI1 oriV by an IncP-1alpha oriV (Aviv et al, 2014;Dionisi et al, 2016). This could be one reason for the controversial findings described above regarding incompatibility of pSI54/04, basically confirming findings of Bogomazova et al (2019) in relation to comparative analysis of a selected set of pESI-like plasmid-bearing strains on the basis of whole-genome sequencing.…”

Section: Discussionmentioning

confidence: 67%

Comparative Genomics of Emerging Lineages and Mobile Resistomes of Contemporary Broiler Strains of Salmonella Infantis and E. coli

2021

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IntroductionCommensal and pathogenic strains of multidrug-resistant (MDR) Escherichia coli and non-typhoid strains of Salmonella represent a growing foodborne threat from foods of poultry origin. MDR strains of Salmonella Infantis and E. coli are frequently isolated from broiler chicks and the simultaneous presence of these two enteric bacterial species would potentially allow the exchange of mobile resistance determinants.ObjectivesIn order to understand possible genomic relations and to obtain a first insight into the potential interplay of resistance genes between enteric bacteria, we compared genomic diversity and mobile resistomes of S. Infantis and E. coli from broiler sources.ResultsThe core genome MLST analysis of 56 S. Infantis and 90 E. coli contemporary strains revealed a high genomic heterogeneity of broiler E. coli. It also allowed the first insight into the genomic diversity of the MDR clone B2 of S. Infantis, which is endemic in Hungary. We also identified new MDR lineages for S. Infantis (ST7081 and ST7082) and for E. coli (ST8702 and ST10088). Comparative analysis of antibiotic resistance genes and plasmid types revealed a relatively narrow interface between the mobile resistomes of E. coli and S. Infantis. The mobile resistance genes tet(A), aadA1, and sul1 were identified at an overall high prevalence in both species. This gene association is characteristic to the plasmid pSI54/04 of the epidemic clone B2 of S. Infantis. Simultaneous presence of these genes and of IncI plasmids of the same subtype in cohabitant caecal strains of E. coli and S. Infantis suggests an important role of these plasmid families in a possible interplay of resistance genes between S. Infantis and E. coli in broilers.ConclusionThis is the first comparative genomic analysis of contemporary broiler strains of S. Infantis and E. coli. The diversity of mobile resistomes suggests that commensal E. coli could be potential reservoirs of resistance for S. Infantis, but so far only a few plasmid types and mobile resistance genes could be considered as potentially exchangeable between these two species. Among these, IncI1 plasmids could make the greatest contribution to the microevolution and genetic interaction between E. coli and S. Infantis.

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Extended-spectrum β-lactamase-producing Salmonella enterica serovar Infantis from humans in Italy

Cited by 6 publications

References 4 publications

Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production

Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production

Comparative genomics of emerging lineages and mobile resistomes of contemporary broiler strains ofSalmonellaInfantis andE. coli

Comparative Genomics of Emerging Lineages and Mobile Resistomes of Contemporary Broiler Strains of Salmonella Infantis and E. coli

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