Extended-spectrum β-lactamase (ESBL) in Danish clinical isolates of Escherichia coli and Klebsiella pneumoniae: Prevalence, β-lactamase distribution, phylogroups, and co-resistance

Hansen, Dennis Schrøder; Schumacher, Helga; Hansen, Frank; Stegger, Marc; Hertz, Frederik Boëtius; Schønning, Kristian; Justesen, Ulrik Stenz; Frimodt‐Møller, Niels

doi:10.3109/00365548.2011.632642

Cited by 44 publications

(28 citation statements)

References 29 publications

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“…In Denmark and many other European Union countries, the most prevalent CTX-M variant among human isolates is CTX-M-15 (60%), followed by CTX-M-14 (12%) and CTX-M-1 (11%) (29). CTX-M-15 is rarely detected in animals (2%) (6), thus indicating that the majority of human infections caused by CTX-M-producing E. coli are not attributable to zoonotic transmission.…”

Section: Discussionmentioning

confidence: 99%

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Hansen

Damborg

Andreasen

et al. 2013

Appl Environ Microbiol

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c Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 g/ml CTX and characterized for the presence of ESBLencoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (bla CTX-M-9 group, compatible with bla CTX-M-14/17 ) and B (bla CTX-M-1 group, compatible with bla CTX-M-1/61 ), whereas only three weaners were positive at farm C (bla CTX-M-1 group, compatible with bla CTX-M-1/61 ). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ϳ10 7 CFU/g in piglets to ϳ10 3 CFU/g in finishers (P < 0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.

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Section: Discussionmentioning

confidence: 99%

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Hansen

Damborg

Andreasen

et al. 2013

Appl Environ Microbiol

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“…the association of bla CTX-M genes with ciprofloxacin and amikacin resistance [44,45]. Co-resistance, as expected, was highest in K. pneumoniae [45], with a single, multi-resistant clone being dominant among the isolates. The co-resistance pattern of the 2 E. coli types was also very different.…”

Section: Discussionmentioning

confidence: 79%

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

Ebrahimi

Mózes²,

Mészáros³

et al. 2014

Chemotherapy

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Aims: We compared the prevalence of extended-spectrum beta-lactamase (ESBL) producers in the faecal samples of 1,109 healthy individuals screened for employment purposes and in 531 asymptomatic individuals applying for long-term care (LTC). Methods: Eosin-methylene blue agar plates supplemented with 2 mg/l cefotaxime were used to determine which individuals were ESBL producers. ESBL phenotype was confirmed by double-disk synergy test and ESBL genes were identified by sequencing. ESBL producers were characterized by co-resistance and integron carriage. Results: ESBL producers were more frequent in the LTC applicants than in the employment screening individuals (7.2 vs. 2.0%; p < 0.0001), with 43 Escherichia coli, 18 Klebsiella pneumoniae, 1 Klebsiella oxytoca and 1 Proteus mirabilis being found. In the employment screening individuals, only E. coli was found. Most ESBL genes (79.4%, 50/63) were bla_CTX-M type; bla_CTX-M-15 was more frequent in the LTC applicants (p < 0.001). Regarding ESBL genes and integron diversity, E. coli isolates from the LTC applicants were more similar to K. pneumoniae than to E. coli from the employment screening individuals. Conclusion: These differences in the characteristics of ESBL producers may represent different sources of colonization. Most LTC applicants harboured K. pneumoniae or E. coli that were probably hospital-acquired whereas the E. coli isolates of many healthy individuals showed similarities to environmental E. coli.

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“…ESBL production was screened for by cefpodoxime resistance analysis and the double-disk synergy test, with disks containing cefotaxime and ceftazidime with or without clavulanate (30). MICs for 18 antibiotics (amoxicillin-clavulanic acid, ampicillin, apramycin, cefotaxime, ceftiofur, chloramphenicol, ciprofloxacin, colistin, florfenicol, gentamicin, meropenem, nalidixic acid, neomycin, spectinomycin, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim) were determined by broth microdilution using a Sensititre system (Trek Diagnostic Systems Ltd., United Kingdom) according to the manufacturer's instructions and Clinical and Laboratory Standards Institute guidelines.…”

Section: Clinical and Epidemiological Information The Who Collaboratingmentioning

confidence: 99%

Temporal Trends in Antimicrobial Resistance and Virulence-Associated Traits within the Escherichia coli Sequence Type 131 Clonal Group and Its H 30 and H 30-Rx Subclones, 1968 to 2012

Olesen

Frimodt-Møller

Leihof

et al. 2014

Antimicrob Agents Chemother

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Extended-spectrum β-lactamase (ESBL) in Danish clinical isolates of Escherichia coli and Klebsiella pneumoniae: Prevalence, β-lactamase distribution, phylogroups, and co-resistance

Cited by 44 publications

References 29 publications

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

Temporal Trends in Antimicrobial Resistance and Virulence-Associated Traits within the Escherichia coli Sequence Type 131 Clonal Group and Its H 30 and H 30-Rx Subclones, 1968 to 2012

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