Extended-spectrum<i>β</i>-Lactamases: Implications for the Clinical Laboratory and Therapy

Harada, Sohei; Ishii, Yoshikazu; Yamaguchi, Keizo

doi:10.3343/kjlm.2008.28.6.401

Cited by 44 publications

(39 citation statements)

References 82 publications

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“…The resistance profiles and antibiotic susceptibility of isolates were determined using the disc diffusion method (Kirby-Bauer) based on Clinical and Laboratory Standards Institute (CLSI) guidelines (13) . The antibiotic discs (MAST, Merseyside, UK) used were imipenem (10µg), amikacin (30µg), gentamicin (10µg), ciprofl oxacin (5µg), ceftazidime (30µg), cefotaxime (30µg), cefpodoxime (10µg), ceftriaxone (30µg), aztreonam (30µg), cefepime (30µg), nalidixic acid (30µg), nitrofurantoin (300µg), co-trimoxazole (25µg), tetracycline (30µg), chloramphenicol (30µg), streptomycin (10µg), and colistin sulfate (25µg).…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Zahedani

Rigi

Bokaeian

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM-and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. Methods: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBLproducing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. Results: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1 , except for two isolates that were bla . The bla SHV types were bla SHV-1 , bla SHV-11 , bla , bla SHV-99 , bla , and bla . Conclusions: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the fi rst report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.

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Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Zahedani

Rigi

Bokaeian

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…These codons did not mutate in DH5α-F8. A previous study reported that mutations at codons 238 and 240 are relevant to the resistance to cefotaxime and ceftazidime (24). The MIC of the original isolates of F8 and F25 showed a high resistant-rate compared to DH5α-F8 and DH5α-F25, which suggested that the original isolates performed other resistant mechanisms leading to the resistance to the 3rd-generation cephalosporin.…”

Section: Discussionmentioning

confidence: 98%

Detection of the SHV genotype polymorphism of the extended-spectrum β-lactamase-producing Gram-negative bacterium

Deng

et al. 2015

Biomedical Reports

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Abstract. The prevalence of extended-spectrum β-lactamases (ESBLs) is due to the extensive usage of the extended-spectrum cephalosporins and leads to huge financial loss worldwide, whilst presenting a challenge to the clinical treatment. The aim of the present study was to delineate the frequency of ESBL occurrence in Enterobacteriaceae and confirm the SHV genotype. A random collection of 153 Escherichia coli isolates (E. coli) and 70 Klebsiella pneumoniae isolates were tested. The amplification products obtained by polymerase chain reaction were sequenced. Isolates with novel mutations were transformed to E. coli DH5α. The minimum inhibitory concentration (MIC) was obtained by a microdilution method. The relevance ratio of ESBL was 67.7% and the proportion of the SHV β-lactamase gene (bla SHV ) was 18.5%. A new genotype of β-lactamase was demonstrated and submitted to GenBank. A total of 12 mutational sites were found in 28 ESBL-producing isolates, including four nonsense mutations. Sensitive-rates of 28 ESBL-producing isolates to imipenem were 100%, and resistant-rates to penicillin, amoxicillin and oxacillin were 100%. The MIC of DH5α-F8 to penicillin, oxacillin, cefoxitin, cefotaxime, cefepime, cefoperazone/sulbactam, imipenem and netilmicin was 512, 512, 2, 0.03, 0.06, 4, 0.015 and 32 respectively. In conclusion, ESBL and SHV-28 is the most prevalent bla. Imipenem is the most effective antibiotic to ESBL, and the 4th-generation cephalosporins and β-lactamase inhibitor compound are also effective. ESBL is mediated by plasmids and able to spread among different Enterobacteriaceae. In conclusion, new mutations of the bla SHV gene exist from at least 2010.

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“…Studies performed in Iraq, Turkey and Iran to detect ESBLs genes using different primer sequences gave amplified products with different molecular weights [15]. The variation in the molecular weights of the amplified products could be attributed to the difference in primer sequences or the type of the gene that detect as currently there are more than 70 SHV and 150 TEM types are recognized [16]. CTX-M are divided into five subgroups having more than 80 enzymes.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq

Assafi¹

2016

Journal of Microbiology and Infectious Diseases

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Objectives: Uropathogenic Escherichia coli are the most important group of microorganisms responsible for urinary tract infection. A high percentage of uropathogenic E. coli over the world are detected to be ESBLs producers, which is now a problem that limits therapeutic options. The aim of this study is to determine the prevalence of ESBLs E. coli and study the prevalence of different ESBLs genotype patterns among the ESBLs producing isolates. Methods:One hundred and six uropathogenic E. coli isolates were analyzed for their ESBL production by molecular methods.Results: 55 (52%) isolates were detected as extended spectrum β-lactamases (ESBLs) producers. Based on the PCR results, all E. coli isolates possessed one or more ESBL gene. CTX-M type ESBL was the most dominant ESBL (87.2%) among the isolates. While those for TEM-type and SHV-type were 54.5% and 21.8% respectively. Six genotype patterns were detected (TEM, CTX-M, TEM+SHV, TEM+CTX-M, SHV+CTX-M and TEM+SHV+CTX-M). The genotype CTX-M was the most prevalent genotype (40%) followed by the genotype TEM+CTX-M combination (30.9%). The occurrences of the genotypes (TEM, TEM+SHV, SHV+CTX-M and TEM+SHV+CTX-M) were 7.3%, 5.5%, 5.4%, 10.9%, respectively. Conclusions:Control measures and education programs are necessary to avoid the uncontrollable use of β-lactam and cephalosporins in order to minimize the emergence of ESBLs producing E. coli. J Microbiol Infect Dis 2016;6(4): 163-167

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Extended-spectrumβ-Lactamases: Implications for the Clinical Laboratory and Therapy

Cited by 44 publications

References 82 publications

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Detection of the SHV genotype polymorphism of the extended-spectrum β-lactamase-producing Gram-negative bacterium

Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq

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