Extended-spectrum and CMY-type b-lactamase-producing Escherichia coli in clinical samples and retail meat from Pittsburgh, USA and Seville, Spain

Abstract

Infections due to Escherichia coli producing extended-spectrum beta-lactamase (ESBL) or CMY-type beta-lactamase (CMY) are increasingly observed in non-hospitalized patients. The origin of these organisms is uncertain, but retail meat contaminated with E. coli may be a source. In the present study, clinical information and strains collected from patients infected or colonized with ESBL-producing and CMY-producing E. coli at hospitals in Pittsburgh, USA and Seville, Spain were investigated. Retail meat purchased in these cities was also studied for the presence of these organisms. Twenty-five and 79 clinical cases with ESBL-producing E. coli and 22 cases and one case with CMY-producing E. coli were identified in Pittsburgh and Seville, respectively. Among them all, community-acquired and healthcare-associated cases together constituted 60% of the cases in Pittsburgh and 73% in Seville. Community-acquired cases were more common in Seville than in Pittsburgh (49% vs. 13%; p <0.001). ESBL-producing and CMY-producing E. coli isolates were commonly recovered from the local retail meat. In particular, 67% (8/12) of retail chickens in Seville and 85% (17/20) of those in Pittsburgh contained ESBL-producing and CMY-producing E. coli isolates, respectively. Among the ESBL-producing isolates, CTX-M and SHV were the most common ESBL types in both clinical and meat isolates. Approximately half of the ESBL-producing and CMY-producing E. coli isolates from meat belonged to phylogenetic groups associated with virulent extra-intestinal infections in humans. Community and healthcare environments are now significant reservoirs of ESBL-producing and CMY-producing E. coli. Retail meat is a potential source of these organisms.

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

“…CTX-M-15, the ESBL considered to have spread in a pandemic fashion in humans, was only detected incidentally in food-producing animals or food and in only in a few countries in E. coli (isolated in France from diseased cattle, in Belgium from healthy poultry, and in UK in broiler chickens and turkey) and in Salmonella isolates (Germany, one S. Typhimurium from horse origin) (Madec et al, 2008;Randall et al, 2011;Rodriguez et al, 2009;Smet et al, 2008). ESBLs of the SHV class were frequently detected throughout the EU, especially SHV-12 and SHV-2 Bortolaia et al, 2010b;Boyle et al, 2010;Brinas et al, 2003a;Brinas et al, 2003b;Chiaretto et al, 2008;Cortes et al, 2010;Costa et al, 2009;Dierikx et al, 2010a;Doi et al, 2009;Escudero et al, 2010;Goncalves et al, 2010;Hasman et al, 2005;Hopkins et al, 2006;Kolar et al, 2010;Machado et al, 2008;Madec et al, 2008;Morris et al, 2009;Riano et al, 2006). In relation to the ESBLs of the TEM class, the most frequently detected throughout the EU was TEM-52 Brinas et al, 2005;Carneiro et al, 2010;Cloeckaert et al, 2007;Costa et al, 2009;Dierikx et al, 2010a;Jensen et al, 2006;Machado et al, 2008;Morris et al, 2009;Randall et al, 2011;Rodriguez et al, 2009;Smet et al, 2008).…”

“…The ESBLs detected in food-producing animals or food in other non-European countries were: China (CTX-M-3, -13, -14, -15, -22, -24, -55, -64, -65; SHV-2, -12; TEM-52), Japan (CTX-M-2 and -14), and Senegal (SHV-12) (Ben Slama et al, 2010;Cardinale et al, 2001;Doi et al, 2009;Duan et al, 2006;Jouini et al, 2007;Kojima et al, 2005;Li et al, 2010a;Li et al, 2010b;Lim et al, 2009;Matsumoto et al, 2007;Rayamajhi et al, 2008;Shiraki et al, 2004;Tian et al, 2008;Wittum et al, 2010). These data show that mainly in Asian countries a higher diversity of CTX-Mvariants was observed.…”

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

“…Isolation of ESBL-resistant enterobacteria in meat and meat products raised questions about the presence of ESBLresistant organisms in food-producing animals Jouini et al, 2007;Hammad et al, 2008;Doi et al, 2010). There have been improvements in isolation of ESBL-resistant enterobacteria in food-producing animals worldwide (Geser et al, 2011).…”

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

“…These data concerns more often the occurrence of the mentioned microorganisms in animals at the farm (3, 4) or the slaughter-house (5, 9), and in less or more processed meat or meat products (8,20,25). The investigations concerning raw meat were performed most often with poultry meat (2,6,28), and more rarely with meat of other animal species (23,32).…”