Extended-spectrum and AmpC β-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: prevalence, risk factors and molecular characteristics

Huijbers, P.M.C.; Graat, E.A.M.; Haenen, Anja; Santen, M G Van; Essen-Zandbergen, Alieda van; Mevius, Dik; Duijkeren, Engeline van; Hoek, Angela H.A.M. van

doi:10.1093/jac/dku178

Cited by 137 publications

(136 citation statements)

References 20 publications

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“…People who worked or lived on a farm were excluded from this study. However, it is already known that broiler and pig farmers and their family members have a higher prevalence of ESBL/pAmpC carriage, and contact with animals was identified as risk factor in two studies [4,6,12]. Prevalence was 27%e33% for broiler farmers [4,6] and 13% for persons living or working on ESBL/pAmpCpositive pig farms [12].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to the other types of animals studied, mink are carnivores; they eat raw residual products from the poultry and fish industry at least once a day [27]. Given the fact that ESBL/pAmpC-E have been frequently found in poultry [2,4,6,17], it is likely that mink carry ESBL/pAmpC-E. It is still unclear if, and how, the presence of mink leads to an increased risk for carriage in the community.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it was unknown which farms were ESBL/pAmpC-positive. In the Netherlands, in the period just before this study (2009e2011), 100% of broiler farms [4,6] and 45% of pig farms were ESBL/pAmpCpositive [12], but for other types of farms, this prevalence is unknown. Finally, selection bias may have occurred due to the twostep selection process of participants.…”

Section: Limitationsmentioning

confidence: 99%

“…Usually, people working on a farm have a higher carriage rate of ESBL/pAmpC-E than the general population [4,6]. Although several studies hypothesize that exposure to ESBL/ pAmpC-E is elevated in close proximity around animal farms [17,18], the risk of this exposure is not clear [14].…”

Section: Introductionmentioning

confidence: 99%

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Extended-spectrum β-lactamase- and pAmpC-producing Enterobacteriaceae among the general population in a livestock-dense area

Wielders

Hoek

Hengeveld

et al. 2017

Clinical Microbiology and Infection

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Extended-spectrum b-lactamases b-lactam resistance Livestock farming Prevalence Risk factors a b s t r a c tObjectives: In the Netherlands there is an ongoing debate regarding environmental health risks of livestock farming for neighbouring residents. This explorative study aims to determine the prevalence of carriage of extended-spectrum b-lactamase and/or plasmid-mediated AmpC-producing Enterobacteriaceae (ESBL/pAmpC-E) in the general population living in a livestock-dense area, and to study associations between determinants, including exposure through contact with animals and the environment, and human carriage of ESBL/pAmpC-E. Methods: A cross-sectional study was performed among 2432 adults (aged 20e72 years) in 12 temporary research centres in the south of the Netherlands, consisting of a questionnaire and analysis of a faecal sample to assess carriage of ESBL/pAmpC-E. Risk factors were analysed using logistic regression. Results: The prevalence for carriage of ESBL/pAmpC-E was 4.5% (109/2432; 95% CI 3.7e5.4) ranging from 1.4% to 10.9% among the research centres. ESBL/pAmpC resistance genes were detected in Escherichia coli and Klebsiella pneumoniae isolates obtained from these 109 persons and the most common ESBLresistance genes were bla CTX-M-15 , bla CTX-M-14/17 and bla CTX-M-1 , originating from 76 participants. Travel in the previous 12 months to Africa, Asia or Latin America (OR 2.82; 95% CI 1.71e4.63), having kept cows for a hobby in the previous 5 years (OR 3.77; 95% CI 1.22e11.64), usage of proton-pump inhibitors (OR 1.84; 95% CI 1.05e3.23), and living within 1000 m of a mink farm (OR 2.26; 95% CI 1.28e3.98) were identified as risk factors. Exposure to poultry was not identified as a risk factor. Conclusions: Overall, living in close proximity to livestock animals and farms does not seem to be a risk factor for carriage of ESBL/pAmpC-E. C.C.H. Wielders, CMI 2017;23:120.e1e120.e8

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Limitationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extended-spectrum β-lactamase- and pAmpC-producing Enterobacteriaceae among the general population in a livestock-dense area

Wielders

Hoek

Hengeveld

et al. 2017

Clinical Microbiology and Infection

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“…ESBLs mediated by plasmids are common in Enterobacteriaceae, particularly in E. coli and K. pneumoniae, which have been reported to cause serious infections in hospitals, as well as in the community in different parts of the world (19,20). A previous study indicated that the present disk diffusion criteria underestimate the prevalence of ESBL-producing strains (21).…”

Section: Discussionmentioning

confidence: 60%

Detection of the SHV genotype polymorphism of the extended-spectrum β-lactamase-producing Gram-negative bacterium

Deng

et al. 2015

Biomedical Reports

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Abstract. The prevalence of extended-spectrum β-lactamases (ESBLs) is due to the extensive usage of the extended-spectrum cephalosporins and leads to huge financial loss worldwide, whilst presenting a challenge to the clinical treatment. The aim of the present study was to delineate the frequency of ESBL occurrence in Enterobacteriaceae and confirm the SHV genotype. A random collection of 153 Escherichia coli isolates (E. coli) and 70 Klebsiella pneumoniae isolates were tested. The amplification products obtained by polymerase chain reaction were sequenced. Isolates with novel mutations were transformed to E. coli DH5α. The minimum inhibitory concentration (MIC) was obtained by a microdilution method. The relevance ratio of ESBL was 67.7% and the proportion of the SHV β-lactamase gene (bla SHV ) was 18.5%. A new genotype of β-lactamase was demonstrated and submitted to GenBank. A total of 12 mutational sites were found in 28 ESBL-producing isolates, including four nonsense mutations. Sensitive-rates of 28 ESBL-producing isolates to imipenem were 100%, and resistant-rates to penicillin, amoxicillin and oxacillin were 100%. The MIC of DH5α-F8 to penicillin, oxacillin, cefoxitin, cefotaxime, cefepime, cefoperazone/sulbactam, imipenem and netilmicin was 512, 512, 2, 0.03, 0.06, 4, 0.015 and 32 respectively. In conclusion, ESBL and SHV-28 is the most prevalent bla. Imipenem is the most effective antibiotic to ESBL, and the 4th-generation cephalosporins and β-lactamase inhibitor compound are also effective. ESBL is mediated by plasmids and able to spread among different Enterobacteriaceae. In conclusion, new mutations of the bla SHV gene exist from at least 2010.

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Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh

Saha

Basri

Hossain

et al. 2023

Veterinary Medicine & Sci

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Background Resistance to multiple drugs in Klebsiella pneumoniae (KPN) is an important issue in human and animal medicine. KPN phenotypic and genotypic aspects in poultry samples have not been comprehensively explored in Bangladesh. Methods This research focused on the prevalence of antibiotic resistance and the characterization of KPN in Bangladeshi poultry isolates using both phenotypic and genotypic approaches. Results A total of 32 poultry samples were randomly obtained from a commercial poultry farm in Narsingdi, Bangladesh, and 43.90% (18/41) of the isolates were confirmed to be KPN, whereas all isolates were biofilm producers. The antibiotic sensitivity test revealed the most remarkable (100%) antibiotic resistance level against Ampicillin, Doxycycline and Tetracycline while remaining susceptible to Doripenem, Meropenem, Cefoxitin and Polymyxin B. Resistance to Nalidixic acid, Nitrofurantoin, Trimethoprim, Levofloxacin, Ciprofloxacin, Cefuroxime and Chloramphenicol ranges from 18% to 70%. Minimum inhibitory concentrations for carbapenem‐resistant KPN ranged from 128 to 512 mg/mL for Meropenem, Imipenem, Gentamycin and Ciprofloxacin, respectively. [Correction added on 15 June 2023, after first online publication: 512 g/mL was corrected to 512 mg/mL in the preceding sentence]. Carbapenemase‐producing KPN isolates harboured a single or multiple β‐lactamase genes, blaSIM‐1, blaIMP‐4 and blaOXA‐48 as well as one ESBL gene (blaTEM) and plasmid‐mediated quinolone resistance gene (qnrB). Furthermore, Cr and Co outperformed Cu and Zn in antibacterial effectiveness. Conclusions The results of this investigation showed that the high prevalence of multidrug‐resistant pathogenic KPN in our chosen geographic location had displayed sensitivity to FOX/PB/Cr/Co, which might be regarded as an alternate treatment to reduce pressure of use on carbapenems.

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Extended-spectrum and AmpC β-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: prevalence, risk factors and molecular characteristics

Cited by 137 publications

References 20 publications

Extended-spectrum β-lactamase- and pAmpC-producing Enterobacteriaceae among the general population in a livestock-dense area

Extended-spectrum β-lactamase- and pAmpC-producing Enterobacteriaceae among the general population in a livestock-dense area

Detection of the SHV genotype polymorphism of the extended-spectrum β-lactamase-producing Gram-negative bacterium

Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh

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