Extended sequence of the turkey MHC B-locus and sequence variation in the highly polymorphic B-G loci

Bauer, Miranda M.; Reed, Kent M.

doi:10.1007/s00251-010-0501-9

Cited by 13 publications

(4 citation statements)

References 61 publications

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“…For example, MHC class I and class II molecules are necessary for antigen presentation to T lymphocytes and are common to the galliform MHC [229][230][231][232][233][234][235][236][237]. In turkeys and chickens, the MHC is composed of 2 genetically unlinked regions, the B-locus (MHC-B) and Y-locus (MHC-Y), co-located on a single microchromosome (GGA16 or MGA18, respectively) [229][230][231][232][233][234][235][236][237]. Although some genes are well characterized, the functions and expression patterns of many poultry MHC genes are still unknown.…”

Section: Cytokines and The Mhcmentioning

confidence: 99%

Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1 in Poultry

2015

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This review is a comprehensive introduction to the effects of poultry exposure to the toxic and carcinogenic mycotoxin aflatoxin B1 (AFB1). The relationship between AFB1 sensitivity and metabolism, major direct and indirect effects of AFB1, recent studies of gene expression and transcriptome responses to exposure, and mitigation strategies to reduce toxicity are discussed. Exposure to AFB1 primarily occurs by consumption of contaminated corn, grain or other feed components. Low levels of residual AFB1 in poultry feeds can cause reduction in growth, feed conversion, egg production, and compromised immune functions, resulting in significant economic costs to producers. Thus, AFB1 acts as a "force multiplier" synergizing the adverse effects of microbial pathogens and other agents, and factors detrimental to poultry health. Domestic turkeys (Meleagris gallopavo) are one of the most sensitive animals known to AFB1 due, in large part, to a combination of efficient hepatic bioactivation by cytochromes P450 1A5 and 3A37, and deficient hepatic glutathione-S-transferase (GST)-mediated detoxification. Because of their sensitivity, turkeys are a good model to investigate chemopreventive treatments and feed additives for their ability to reduce AFB1 toxicity. Transcriptome analysis (RNA-seq) of turkey poults (liver and spleen) has identified AFB1-induced gene expression changes in pathways of apoptosis, carcinogenesis, lipid regulation, antimicrobial activity, cytotoxicity and antigen presentation. Current research focuses on further identifying the molecular mechanisms OPEN ACCESSAgriculture 2015, 5 743 underlying AFB1 toxicity with the goal of reducing aflatoxicosis and improving poultry health.

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Section: Cytokines and The Mhcmentioning

confidence: 99%

Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1 in Poultry

2015

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“…CTL kill transformed cells and viral infected cells, preventing replication of tumor cells or release of the pathogen [11]. The intracellular loading of newly synthesized Class I MHC molecules with peptides in the ER requires the co-operative function of several co-factors, including the TAP, calnexin, calreticulin, Erp57, and the dedicated chaperone Tapasin [12].…”

Section: Discussionmentioning

confidence: 99%

Identification and sequence analysis of Tapasin gene in guinea fowl

Panicker¹,

Uma²

2014

Vet World

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Aim: An attempt has been made to identify and study the nucleotide sequence variability in exon 5 -exon 6 regions of guinea fowl Tapasin gene.Materials and Methods: Blood samples were collected from randomly selected birds (12 guinea fowl birds) and Tapasin gene amplified using chicken specific primers designed from GenBank submitted sequences. Polymerase chain reaction conditions were standardized so as get only single amplicons. Obtained products were then cloned and sequenced; sequences were then analyzed using suitable software.Results: Amplicon size of the Tapasin gene in guinea fowl was same as reported in chicken with areas of transitions and transversions. The sequence variations reported in these coding sequences might have influence in the protein structure, which may be correlated with the increased immune status of the bird when compared with chicken breeds. Conclusion:Since Tapasin gene is an immunologically important gene, which plays an important role in the immune status of the bird. Sequence variations in the gene can be correlated with the altered immune status of the bird.

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“…Primers could be designed [Chaves et al, 2009;Bauer and Reed, 2011]. Gene families are similarly colored, and vertical lines connect homologs.…”

Section: Resultsmentioning

confidence: 99%

Conserved MHC Gene Orthologs Genetically Map to the Turkey MHC-<b><i>B</i></b>

Reed

Benoit

Wang

et al. 2014

Cytogenet Genome Res

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The avian MHC-associated gene set includes orthologs to genes found throughout the human major histocompatibility complex (MHC), including some loci of the evolutionarily conserved class III region. In the turkey and other Galliformes, genes linked to the MHC have been identified because they are closely associated with class I or class II genes. This study was designed to evaluate additional class III genes for linkage to the avian MHC to further determine conservation of these loci in birds. BLAST searches were used to locate sequences in the turkey genome with similarity to genes shared between the MHC of Xenopus and humans. Primers were designed to target 25 genes, and putative orthologs were amplified by PCR and sequenced. Sequence polymorphisms were identified for 15 genes in turkey reference mapping families, and 8 genes showed significant genetic linkage to the turkey MHC-B locus. These new genetic markers and linkage relationships broaden our understanding of the composition of the avian MHC and expand the gene content for the turkey MHC-B.

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Extended sequence of the turkey MHC B-locus and sequence variation in the highly polymorphic B-G loci

Cited by 13 publications

References 61 publications

Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1 in Poultry

Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1 in Poultry

Identification and sequence analysis of Tapasin gene in guinea fowl

Conserved MHC Gene Orthologs Genetically Map to the Turkey MHC-<b><i>B</i></b>

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