Extended Förster theory: a quantitative approach to the determination of inter-chromophore distances in biomacromolecules

Abstract

This review highlights recent theoretical and experimental advances in the study of biomacromolecular structure by using electronic transfer. The considered electronic transport in the extended Förster theory occurs within donor-acceptor pairs, donor-donor pairs, as well as within regular arrangements of many donors which may undergo reorienting and translational dynamics. The classical and the extended Förster theory are compared. Applications concern the determination of structural properties of proteins and non-covalent protein polymers. Studies of energy migration by means of two-photon excited fluorescence spectroscopy, as well as the relevant extension of the Förster theory are presented.

On the analyses of fluorescence depolarisation data in the presence of electronic energy migration. Part I: Theory and general description

“…One should notice that previously published equations for the TPE anisotropy in the presence of energy migration (4,22) are only justified for this particular case. The general expressions for (2) ij ( ) r t  are more complex (cf.…”

“…Unlike OPE depolarisation experiments, linearly as well as circularly polarised two-photon excitation experiments provide additional information. Actually, OPE and TPE experiments are independent and importantly, they provide the same molecular information about reorienting motions(9-10) and energy migration processes (4). TPE donor-acceptor electronic energy transfer is increasingly used in life sciences (11).…”

“…To overcome these limitations an extended Förster theory (EFT) has been developed(3) and applied on various chemical systems (4). The EFT provides a new tool for examining biomacromolecular structure, which can complement established structural methods.…”

On the analyses of fluorescence depolarisation data in the presence of electronic energy migration. Part II: Applying and evaluating two-photon excited fluorescence

“…According to the EFT of DDEM within pairs of identical fluorescent groups, the fluorescence anisotropy can be written as: 10,17 rðtÞ ¼ 1 2 In eqn (2) h. . .i denotes an ensemble average over all absorbing/ emitting DD-pairs, ra,ij (t) is the probability that a photon with a given polarisation is absorbed by the i th fluorophore and then followed by the emission of a photon with the polarisation a from the j th fluorophore.…”

Förster resonance energy transfer and excited state life time reduction of rhodamine 6G with NiO nanorods in PVP films

“…The emission energy of a donor molecule is extracted by an acceptor molecule by non-radiative and non-electron transfer phenomena and through the dipole -dipole interactions [8]. Coupling leads to efficient energy harvesting, light emitting, bio imaging and bio sensing devices [9].…”