Extended FMRFamides in dipteran insects: Conservative expression in the neuroendocrine system is accompanied by rapid sequence evolution

Rahman, M. M.; Fromm, Bastian; Neupert, Susanne; Kreusch, Stefan; Predel, Reinhard

doi:10.1016/j.ygcen.2008.08.006

Cited by 14 publications

(13 citation statements)

References 48 publications

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“…In extracts of nervous tissues from D. suzukii, FMRFamides were only found in the brain and TAG, and do not appear to be present in the CC of this insect. Similarly, previous reports identified FMRFamide-like peptides throughout the CNS of the cabbage root fly (Delia radicum), the house fly (Musca domestica), the sheep blowfly (Lucilia cuprina), the blue bottle (Calliphora vomitoria) and the grey flesh fly (Sarcophaga bullata), and were not detected in the CC [5,46,52].…”

Section: Discussionsupporting

confidence: 74%

“…This tetrapeptide itself is not present in insects, only N-terminally extended FMRFamides have been identified to date. Furthermore, although a variable number of peptides are predicted from the FMRFamide-like peptide precursors of insects, it is only in flies [5,46] and aphids [16,29] where peptides with a C-terminal FMRFamide motif have been identified. In extracts of nervous tissues from D. suzukii, FMRFamides were only found in the brain and TAG, and do not appear to be present in the CC of this insect.…”

Section: Discussionmentioning

confidence: 99%

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Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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Tel: +44 (0)1904 4626282 Highlights  In silico analysis identified 28 peptide precursors  Peptidome analysis of adult brain, corpus cardiacum, thoracico-abdominal ganglion identified peptides from 15 different families.  Myosuppressin was present in all tissue analysed, and was the only peptide identified in the crop nerve bundle AbstractDrosophila suzukii is a highly polyphagous invasive pest which has been recently introduced into Europe and North America, where it is causing severe economic losses through larval infestations of stone and berry fruits. The peptidome of the selected nervous tissues of adult D. suzukii was investigated as a first step in identifying potential targets for the development of novel insecticides.Through in silico analyses of the D. suzukii genome databases 28 neuropeptide families, comprising more than 72 predicted peptides were identified. Using a combination of liquid chromatography and mass spectrometry of tissue extracts, 33 predicted peptides, representing 13 different peptide families were identified by their molecular masses and a total of 17 peptide sequences were confirmed by ion fragmentation.A comparison between the peptides and precursors of D. suzukii and D. melanogaster shows they are highly conserved, with differences only identified in the amino acid sequences of the peptides encoded in the FMRFamide, hugin and ecydysis triggering hormone precursors. All other peptides predicted and identified from D. suzukii appear to be identical to those previously characterized from D. melanogaster.Adipokinetic hormone was only identified in the corpus cardiacum, other peptides present included short neuropeptide F, a pyrokinin and myosuppressin, the latter of which was the only peptide identified from the crop nerve bundle. Peptides present in extracts of the brain and/or thoracicoabdominal ganglion included allatostatins, cardioacceleratory peptide 2b, corazonin, extended FMRFamides, pyrokinins, myoinihibitory peptides, neuropeptide-like precursor 1, SIFamide, short neuropeptide F, kinin, sulfakinins and tachykinin related peptides.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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“…The FaLPs are distributed over the central and peripheral nervous system and may perform diverse roles as neurohormones, neuromodulators, or endocrine factors acting on the gut (Tsang and Orchard 1991). Recent peptidomic studies identified different FaLPs in flies (Rahman et al 2009;, locusts ), true bugs (Ons et al 2009;Huybrechts et al 2010), beetles (Li et al 2008;Marciniak and Rosinski 2010), and wasps (Hauser et al 2010) and their physiological functions have been subject to a number of studies.…”

Section: Fmrfamide-like Peptidesmentioning

confidence: 99%

Peptidergic control of food intake and digestion in insects¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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Like all heterotrophic organisms, insects require a strict control of food intake and efficient digestion of food into nutrients to maintain homeostasis and to fulfill physiological tasks. Feeding and digestion are steered by both external and internal signals that are transduced by a multitude of regulatory factors, delivered either by neurons innervating the gut or mouthparts, or by midgut endocrine cells. The present review gives an overview of peptide regulators known to control feeding and digestion in insects. We describe the discovery and functional role in these processes for insect allatoregulatory peptides, diuretic hormones, FMRFamide-related peptides, (short) neuropeptide F, proctolin, saliva production stimulating peptides, kinins, and tachykinins. These peptides control either gut myoactivity, food intake, and (or) release of digestive enzymes. Some peptides exert their action at multiple levels, possibly having a biological function that depends on their site of delivery. Many regulatory peptides have been physically extracted from different insect species. However, multiple peptidomics, proteomics, transcriptomics, and genome sequencing projects have led to increased discovery and prediction of peptide (precursor) and receptor sequences. In combination with physiological experiments, these large-scale projects have already led to important steps forward in unraveling the physiology of feeding and digestion in insects.Key words: insect, midgut, digestion, neuropeptides, stomatogastric nervous system. Résumé : Comme tous les organismes hétérotrophes, les insectes requièrent un contrôle strict de leur ingestion de nourriture et la digestion de leur nourriture en nutriments afin de maintenir leur homéostasie et de compléter leurs tâches physiologiques. L'alimentation et la digestion sont gouvernées par des signaux tant externes qu'internes qui sont transmis par un multitude de facteurs régulateurs, livrés ou bien par les neurones qui innervent le tube digestif ou les pièces buccales ou alors par les cellules endocrines du tube digestif moyen. Notre revue fournit une synthèse des régulateurs peptidiques connus pour contrôler l'alimentation et la digestion chez les insectes. Nous décrivons la découverte et le rôle fonctionnel de ces processus dans le cas des peptides allatorégulateurs, des hormones diurétiques, des peptides apparentés aux amides FMRF, du neuropeptide F (court), de la proctoline, des peptides stimulateurs de la production de salive, des kinines et des tachykinines des insectes. Ces peptides contrôlent la myoactivité du tube digestif, l'ingestion de nourriture et(ou) la libération des enzymes digestives. Certains peptides agissent à de multiples niveaux, possédant une fonction biologique qui dépend de leur site d'action. Plusieurs peptides régulateurs ont été extraits physiquement de différentes espèces d'insectes. Cependant, plusieurs projets de peptidomique, de protéomique, de transcriptomique et de génomique ont mené à de nombreuses découvertes supplémentaires et des prédict...

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“…There are over 50 deduced or isolated insect FLPs, examples are shown in Table 10, and from this it can be seen that FLPs are particularly diverse in flies (diptera) (Rahman et al 2009). Unlike the situation in crustacean, the main C-terminal FLP in insects is MRFa, followed by IRFa and then LRFa.…”

Section: Arthropodsmentioning

confidence: 99%

“…This immunoreactivity has been found in all types of neurones and immunoreactive axons extend to the corpora cardiaca and to tissues including the heart and somatic muscle. Extended FMRFas are expressed throughout the nervous system and are the most abundant neuropeptides in thoracic neuroendocrine cells (Predel and Neupert 2007;Rahman et al 2009). …”

Section: Arthropodsmentioning

confidence: 99%

A review of FMRFamide- and RFamide-like peptides in metazoa

2009

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Neuropeptides are a diverse class of signalling molecules that are widely employed as neurotransmitters and neuromodulators in animals, both invertebrate and vertebrate. However, despite their fundamental importance to animal physiology and behaviour, they are much less well understood than the small molecule neurotransmitters. The neuropeptides are classified into families according to similarities in their peptide sequence; and on this basis, the FMRFamide and RFamide-like peptides, first discovered in molluscs, are an example of a family that is conserved throughout the animal phyla. In this review, the literature on these neuropeptides has been consolidated with a particular emphasis on allowing a comparison between data sets in phyla as diverse as coelenterates and mammals. The intention is that this focus on the structure and functional aspects of FMRFamide and RFamide-like neuropeptides will inform understanding of conserved principles and distinct properties of signalling across the animal phyla.

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Extended FMRFamides in dipteran insects: Conservative expression in the neuroendocrine system is accompanied by rapid sequence evolution

Cited by 14 publications

References 48 publications

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

A review of FMRFamide- and RFamide-like peptides in metazoa

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