Abstract:Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails to capture effectively the fluorescence signal generated by the illumination light sheets, in imaging large volumes. Here, we present ExD-SPIM (extended depthof-field selective-plane illumination microscopy), an improved light-sheet microscopy strategy that solves this limita… Show more
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