Expulsion of selenium/protein nanoparticles through vesicle-like structures by Saccharomyces cerevisiae under microaerophilic environment

Zhang, Liang; Li, Daping; Gao, Ping

doi:10.1007/s11274-012-1150-y

Cited by 45 publications

(37 citation statements)

References 23 publications

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“…The most abundant modification was then searched separately in database searches as variable modifications to ensure full coverages. All modifications were restricted to a false discovery rate 1% at the peptide and protein levels and annotation score ( A ) > 20 . LFQ modification outputs were combined and processed as described using R, with contaminants, and reverse identification excluded from further data analysis.…”

Section: Methodsmentioning

confidence: 99%

“…All modifications were restricted to a false discovery rate 1% at the peptide and protein levels and annotation score (A) > 20. [29] LFQ modification outputs were combined and processed as described using R, with contaminants, and reverse identification excluded from further data analysis. LFQ ratio between modified and unmodified peptides was calculated and log2 transformed as well as non-zero quantitation value in more than half of the samples was required for further analysis.…”

Section: Protein Post-translational Modifications Identification and mentioning

confidence: 99%

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Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

et al. 2019

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Issues associated with upscaling exosome production for therapeutic use may be overcome through utilizing artificial exosomes. Cell‐derived mimetic nanovesicles (M‐NVs) are a potentially promising alternative to exosomes for clinical applicability, demonstrating higher yield without incumbent production and isolation issues. Although several studies have shown that M‐NVs have similar morphology, size and therapeutic potential compared to exosomes, comprehensive characterization and to what extent M‐NVs components mimic exosomes remain elusive. M‐NVs were generated through the extrusion of cells and proteomic profiling demonstrated an enrichment of proteins associated with membrane and cytosolic components. The proteomic data herein reveal a subset of proteins that are highly abundant in M‐NVs in comparison to exosomes. M‐NVs contain proteins that largely represent the parental cell proteome, whereas the profile of exosomal proteins highlight their endosomally derived origin. This advantage of M‐NVs alleviates the necessity of endosomal sorting of endogenous therapeutic proteins or RNA into exosomes. This study also highlights differences in protein post‐translational modifications among M‐NVs, as distinct from exosomes. Overall this study provides key insights into defining the proteome composition of M‐NVs as a distinct from exosomes, and the potential advantage of M‐NVs as an alternative nanocarrier when spontaneous endosomal sorting of therapeutics are limited.

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Section: Methodsmentioning

confidence: 99%

Section: Protein Post-translational Modifications Identification and mentioning

confidence: 99%

Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

et al. 2019

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“…The product from dissimilatory reduction of selenite is generally Se 0 , which appears in the form of Se nanoparticles. Microbial reduction occurs either in the periplasmic space (intracellularly) (23)(24)(25) or extracellularly (26)(27)(28). The reduction of Se oxyanions to Se 0 nanoparticles can also be mediated aerobically by diverse species of bacteria, namely, selenium-resistant bacteria (29)(30)(31)(32).…”

Section: ϫmentioning

confidence: 99%

Microbial Transformations of Selenium Species of Relevance to Bioremediation

Eswayah

Smith

Gardiner

2016

Appl Environ Microbiol

173

102

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b Selenium species, particularly the oxyanions selenite (SeO 3 2؊ ) and selenate (SeO 4 2؊ ), are significant pollutants in the environment that leach from rocks and are released by anthropogenic activities. Selenium is also an essential micronutrient for organisms across the tree of life, including microorganisms and human beings, particularly because of its presence in the 21st genetically encoded amino acid, selenocysteine. Environmental microorganisms are known to be capable of a range of transformations of selenium species, including reduction, methylation, oxidation, and demethylation. Assimilatory reduction of selenium species is necessary for the synthesis of selenoproteins. Dissimilatory reduction of selenate is known to support the anaerobic respiration of a number of microorganisms, and the dissimilatory reduction of soluble selenate and selenite to nanoparticulate elemental selenium greatly reduces the toxicity and bioavailability of selenium and has a major role in bioremediation and potentially in the production of selenium nanospheres for technological applications. Also, microbial methylation after reduction of Se oxyanions is another potentially effective detoxification process if limitations with low reaction rates and capture of the volatile methylated selenium species can be overcome. This review discusses microbial transformations of different forms of Se in an environmental context, with special emphasis on bioremediation of Se pollution. Since the discovery in 1954 by Pinsent that the oxidation of formate by cell suspensions of Escherichia coli requires growth medium containing molybdate and selenite, there has been a growing interest in the biochemical role of selenium in microorganisms (1). Se is an essential component of selenoamino acids, such as selenomethionine and selenocysteine (the 21st proteinogenic amino acid), that occur in certain types of prokaryotic enzymes. Indeed, the requirement for selenite in E. coli growing on formate is linked to the fact that formate dehydrogenase contains selenocysteine. Other prokaryotic enzymes that contain selenocysteine include glycine reductase in several clostridia, formate dehydrogenases in diverse prokaryotes, including Salmonella, Clostridium, and Methanococcus, as well as hydrogenases in Methanococcus and other anaerobes. In addition, other bacterial Se-dependent enzymes, in which the selenium is part of the active site molybdenum-containing cofactor, include nicotinic acid dehydrogenase and xanthine dehydrogenase, which is present in certain clostridial species (2-4).Reactions that are involved in the cycling of Se in soil, including those influenced by microbes, are diagrammatically summarized in Fig. 1 2Ϫ and was spatially separated from sulfate reduction in the environment despite the presence of substantial concentrations of sulfate where it occurred. Thus, it can be concluded that Se and S have different reductive biogeochemical cycles and appear to involve distinct populations of microorganisms.With respect to the remediation of sel...

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“…Thus, the common link is that Se specification in nature is strongly dependent on microbial activity 2122. The formation of Se(0) nanoparticles (20-300 nm in diameter) by selenium-respiring bacteria and yeast is a phenomenon that deserves special mention 1923. They occur outside the cell envelope, eventually slough off the cell surface and get released into the medium.…”

Section: Se Compounds In Naturementioning

confidence: 99%

Yeast as a model system to study metabolic impact of selenium compounds

Herrero¹,

Wellinger²

2015

MIC

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Inorganic Se forms such as selenate or selenite (the two more abundant forms in nature) can be toxic in Saccharomyces cerevisiae cells, which constitute an adequate model to study such toxicity at the molecular level and the functions participating in protection against Se compounds. Those Se forms enter the yeast cell through other oxyanion transporters. Once inside the cell, inorganic Se forms may be converted into selenide through a reductive pathway that in physiological conditions involves reduced glutathione with its consequent oxidation into diglutathione and alteration of the cellular redox buffering capacity. Selenide can subsequently be converted by molecular oxygen into elemental Se, with production of superoxide anions and other reactive oxygen species. Overall, these events result in DNA damage and dose-dependent reversible or irreversible protein oxidation, although additional oxidation of other cellular macromolecules cannot be discarded. Stress-adaptation pathways are essential for efficient Se detoxification, while activation of DNA damage checkpoint and repair pathways protects against Se-mediated genotoxicity. We propose that yeast may be used to improve our knowledge on the impact of Se on metal homeostasis, the identification of Se-targets at the DNA and protein levels, and to gain more insights into the mechanism of Se-mediated apoptosis.

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Expulsion of selenium/protein nanoparticles through vesicle-like structures by Saccharomyces cerevisiae under microaerophilic environment

Cited by 45 publications

References 23 publications

Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

Microbial Transformations of Selenium Species of Relevance to Bioremediation

Yeast as a model system to study metabolic impact of selenium compounds

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