Expressions of Senescence-Associated β-Galactosidase and Senescence Marker Protein-30 are Associated with Lens Epithelial Cell Apoptosis

Zhou, Dajun; Yin, Dan; Xiao, Fang; Hao, Jie

doi:10.12659/msm.895596

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…The association between ARC formation and aging markers has been reported [29,30], but the specific mechanism by which cellular senescence causes cataract remains largely unknown. In the present study, we explored whether Met treatment could attenuate human lens epithelial B3 cells (HLE-B3) senescence due to H 2 O 2 exposure.…”

Section: Introductionmentioning

confidence: 99%

Metformin Prevents H₂O₂-Induced Senescence in Human Lens Epithelial B3 Cells

Zhang

Chen

Zhou

et al. 2020

Med Sci Monit Basic Res

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Background: The primary purpose of this study was to investigate the protective effect of metformin against hydrogen peroxide (H 2 O 2)-induced cellular senescence and to explore the underlying molecular mechanism of lens epithelial cell senescence. Material/Methods: We used H 2 O 2 to establish senescence in human lens epithelial B3 cells. The cells were exposed to H 2 O 2 for different numbers of days to mimic aging. Senescence was assessed by senescence-associated b-galactosidase staining, and the molecular mechanism was assessed by real-time polymerase chain reaction (RT-PCR) and western blot analysis. The cultured cells were exposed to 150 μM H 2 O 2 for 7 days with or without metformin to detect the underlying molecular mechanism of lens epithelial cell senescence. Results: The lens epithelial cells exposed to 150 µM H 2 O 2 for 7 days exhibited senescence. The expression levels of senescence-related markers were increased in H 2 O 2-treated cells. Metformin prevented H 2 O 2-induced cellular senescence in human lens epithelial B3 cells. Conclusions: These findings suggest that senescence marker expression is increased in the cells exposed to H 2 O 2. Metformin protects human lens epithelial B3 cells from H 2 O 2-induced senescence.

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Section: Introductionmentioning

confidence: 99%

Metformin Prevents H₂O₂-Induced Senescence in Human Lens Epithelial B3 Cells

Zhang

Chen

Zhou

et al. 2020

Med Sci Monit Basic Res

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“…We have shown further that the number of senescent cells induced by UA is greater than those committed apoptosis, suggesting that cell senescence is the major process of cellular damages in the human lens epithelial cells. These cellular damages may underlie the apoptosis and senescence that were detected in epithelial cells of anterior lens capsule, particularly in the central regions, taken from lens with age‐related cataract 32 . UA is also shown to promote epithelial‐mesenchymal transition in renal tubular cells and induce expansion of myofibroblast population from fibroblasts 33,34 .…”

Section: Discussionmentioning

confidence: 99%

“…These cellular damages may underlie the apoptosis and senescence that were detected in epithelial cells of anterior lens capsule, particularly in the central regions, taken from lens with agerelated cataract. 32 UA is also shown to promote epithelial-mesenchymal transition in renal tubular cells and induce expansion of myofibroblast population from fibroblasts. 33,34 These findings together with current results propose that the elevated level of UA in aqueous humour might induce local and chronic oxidative stress to the lens epithelial cells, which leads to senescence and apoptosis in the anterior lens epithelial cells and mesenchymal transition of the equatorial lens cells that are associated with the pathogenesis of PSC.…”

Section: Discussionmentioning

confidence: 99%

Elevated level of uric acid in aqueous humour is associated with posterior subcapsular cataract in human lens

Qin

Chan

Lin

et al. 2020

Clinical Exper Ophthalmology

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Importance Age‐related cataract is the leading cause of blindness worldwide. The pathological mechanisms causing this disease remain elusive. Background To examine the involvement of uric acid (UA) in the pathogenesis of posterior subcapsular cataract (PSC). Design Retrospective study and experimental investigation. Participants A total of 180 patients with PSC or non‐PSC were included. Methods Samples obtained from the patients were used to analyse content of UA and for histochemical examinations. The effects of UA on human lens epithelial cells were also investigated. Main Outcome Measures Aqueous humour UA and urate deposits. Results The results showed a significant increase of aqueous humour UA in patients with PSC. After adjustment for potential confounders, elevated aqueous humour UA (odds ratio [OR] = 1.45) showed a stronger association with PSC than serum UA (OR = 1.10). Gomori methenamine silver staining revealed in PSC an intense deposit of urates in the lens fibres in equatorial regions, and in subcapsular fibres in posterior regions of the lens. Such staining was not detected in the lens with non‐PSC. Treatment with UA‐induced senescence and apoptosis in human lens epithelial cells in a dose dependent manner. Our results suggest that the elevated level of UA in aqueous humour causes a deposition of urates in human lens epithelium, which could possibly lead to dysfunction of these cells that generates opacification in PSC. Conclusions and Relevance These findings indicate the local action of excessive UA in the pathogenesis of PSC. Control of serum UA level could delay the progression of PSC.

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“…For reference, the intracellular Ca 2+ homeostasis was related to cell death through the action of the proteolytic enzymes. Additionally, the intracellular targets of apoptotic Ca 2+ signal was with the special emphasis of the mitochondria on the cytosolic Ca 2+ -dependent enzymes [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Red Algae “Sarcodia suieae” Acetyl-Xylogalactan Downregulate Heat-Induced Macrophage Stress Factors Ddit3 and Hyou1 Compared to the Aquatic Animal Model of Nile Tilapia (Oreochromis niloticus) Brain Arachidonic Acid Expression

Pan

Wang

Nan

et al. 2022

IJMS

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Anthropogenic climate change is known to be an increased stress that affects aquatic animal behavior and physiological alternations, which can induce the animal’s death. In order to known whether the extracted acetyl-xylogalactan function on the regulation of the external high temperature induced death, we first selected the mammalian cell line “RAW 264.7” used in the previous experiment to evaluate the extracted acetyl-xylogalactan function. We aimed to evaluate the effects of the acetyl-xylogalactan on the RAW 264.7 macrophages and Nile Tilapia stress factor expression under the heat environment. In the in vitro cell observation, we assessed the cell survival, phagocytic activity, intracellular Ca2+ level, mitochondria potential exchange, apoptotic assay findings, galactosidase activity, RNA-seq by NGS and real-time polymerase chain reaction (QPCR) expression. In the in vivo Nile Tilapia observation aimed to evaluate the blood biochemical indicator, brain metabolites exchange and the liver morphology. In our evaluation of RAW 264.7 macrophages, the RNA sequencing and real-time polymerase chain reaction (PCR) was shown to upregulate the expression of the anti-apoptosis Cflar gene and downregulate the expression of the apoptosis factors Ddit3 and Hyou1 to protect macrophages under heat stress. We already knew the extracted acetyl-xylogalactan function on the mammalian “RAW 264.7” system. Following, we used the aquatic Nile Tilapia model as the anthropogenic climate change high temperature experiment. After feeding the Nile Tilapia with the acetyl-xylogalactan, it was found to reduce the brain arachidonic acid (AA) production, which is related to the NF-κB-induced apoptosis mechanism. Combined with the in vitro and in vivo findings, the acetyl-xylogalactan was able to reduce the heat induced cell or tissue stress.

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Expressions of Senescence-Associated β-Galactosidase and Senescence Marker Protein-30 are Associated with Lens Epithelial Cell Apoptosis

Cited by 12 publications

References 44 publications

Metformin Prevents H₂O₂-Induced Senescence in Human Lens Epithelial B3 Cells

Metformin Prevents H₂O₂-Induced Senescence in Human Lens Epithelial B3 Cells

Elevated level of uric acid in aqueous humour is associated with posterior subcapsular cataract in human lens

Red Algae “Sarcodia suieae” Acetyl-Xylogalactan Downregulate Heat-Induced Macrophage Stress Factors Ddit3 and Hyou1 Compared to the Aquatic Animal Model of Nile Tilapia (Oreochromis niloticus) Brain Arachidonic Acid Expression

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