Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide

Lee, Kiho

doi:10.12717/dr.2014.18.4.293

Cited by 7 publications

(15 citation statements)

References 14 publications

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“…The differential expression of Cx isoforms in the epididymis during postnatal period has been demonstrated by our previous and other researches (Dufresne et al, 2003; Han & Lee, 2013; Lee 2013; Pointis et al, 2005). Moreover, our recent findings have demonstrated that expression of Cx isoforms in the initial segment and corpus epididymis is differentially regulated by estradiol benzoate, an agonist of estradiol, or flutamide, a synthetic antiandrogen, which is administrated at the early neonatal and/or weaning age (Lee, 2014; Lee & Lee, 2015). However, the effect of estradiol benzoate or flutamide on the expression of Cx isoforms in other epididymal regions has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Lee¹

2016

Development &Amp; Reproduction

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Direct communication between neighboring cells via gap junction in tissue is important for maintenance and regulation of its physiological functions. Each epididymal region has different composition of cell types. It is well recognized that the epididymis is a steroid hormone-responsive tissue. The present study was designed to determine the effect of estradiol benzoate (EB) or flutamide exposured at the early postnatal age on the expression of connexin (Cx) isoforms in the caudal epididymis. The EB or flutamide was subcutaneously administrated to male Spragure Dawley rat at 7 days of age, and expressional changes of Cx isoforms in the adult corpus epididymis were determined by quantitative real-time PCR. The treatment of low-dose EB resulted in decreases of Cx30.3, Cx31.1, Cx37, and Cx45 expression but caused an increase of Cx32 expression. Exposure to high-dose EB led into expressional increases of Cx31, Cx31.1, Cx32, Cx40, and Cx43, even though a decrease of Cx37 expression was found with a high-dose EB treatment. A low-dose flutamide induced increases of Cx31, Cx31.1, Cx32, and Cx43 expression but a decrease of Cx37 expression. Expression of most Cx genes were significantly increased by a high-dose flutamide, while no expressional change of Cx26 and Cx40 was detected by a high-dose flutamide. These results indicate that expression of Cx isoforms in the caudal epididymis is altered by exposure to steroidal compounds at the prepubertal age. It is suggested that a contact with environmental exogenous materials during the early postnatal period would lead to alteration of epididymal functions at the adult.

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Section: Introductionmentioning

confidence: 99%

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Lee¹

2016

Development &Amp; Reproduction

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“…St-Pierre et al (2003) have shown the drastic decrease of Cx 43 expression in the initial segment, caput epididymis, and corpus epididymis, but not in the caudal epididymis by propylthiouracil-induced hypothyroidism at the neonatal age. Exposure to estradiol benzoate (EB), an estrogen agonist, or flutamide (Flu), an antiandrogenic compound, at the neonatal or weaning age results in aberrant expression of several Cx isoforms in the initial segment and corpus epididymis of the adult rat (Lee, 2014, 2015a, Lee, 2015b). However, it is notable that the expressional changes of Cx isoforms induced by EB or Flu treatment are different in each epididymal segment and/or a type of the treatment (Lee, 2014, 2015a, 2015b).…”

Section: Introductionmentioning

confidence: 99%

“…Exposure to estradiol benzoate (EB), an estrogen agonist, or flutamide (Flu), an antiandrogenic compound, at the neonatal or weaning age results in aberrant expression of several Cx isoforms in the initial segment and corpus epididymis of the adult rat (Lee, 2014, 2015a, Lee, 2015b). However, it is notable that the expressional changes of Cx isoforms induced by EB or Flu treatment are different in each epididymal segment and/or a type of the treatment (Lee, 2014, 2015a, 2015b). Moreover, expressional patterns of Cx isoforms in a given region of the adult epididymis differ with the time of exposure to EB or Flu during postnatal period (Lee, 2014, 2015a).…”

Section: Introductionmentioning

confidence: 99%

“…However, it is notable that the expressional changes of Cx isoforms induced by EB or Flu treatment are different in each epididymal segment and/or a type of the treatment (Lee, 2014, 2015a, 2015b). Moreover, expressional patterns of Cx isoforms in a given region of the adult epididymis differ with the time of exposure to EB or Flu during postnatal period (Lee, 2014, 2015a). Most recently, our research has demonstrated expressional changes of Cx isoforms in the adult caudal epididymis administrated with EB or Flu at the early neonatal age (Lee, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Exposure to Estradiol Benzoate or Flutamide at the Weaning Age on Expression of Connexins in the Caudal Epididymis of Adult Rat

Lee

2016

Dev Reprod

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The present research was chiefly designed to determine the effect of the treatment of estrogenic agonist, estradiol benzoate (EB), or antiandrogenic compound, flutamide (Flu), at the weaning age on the expression of connexin (Cx) isoforms in the caudal epididymis of adult male rat. Animals were subcutaneously administrated with a single shot of either EB at a low-dose (0.015 µg of EB/kg body weight (BW)) or a high-dose (1.5 µg of EB/kg BW) or Flu at a low-dose (500 µg of EB/kg BW) or a high-dose (5 mg of EB/kg BW). Expressional changes of Cx isoforms in the adult caudal epididymis were examined by quantitative real-time PCR analysis. The treatment of a low-dose EB caused significant increases of Cx30.3, Cx31, Cx32, and Cx43 transcript levels but reduction of Cx31.1, Cx37, and Cx45 expression. Exposure to a high-dose EB resulted in very close responses observed in a low-dose EB treatment, except no significant expressional change of Cx37 and a significant induction of Cx40. Expression of all Cx isoforms, except Cx45, was significantly increased by a low-dose Flu treatment. Expressional increases of all Cx isoforms were detected by a high-dose Flu treatment. The current study demonstrates that a single exposure to estrogenic or antiandrogenic compound during the early postnatal developmental period is sufficient to disrupt normal expression of Cx isoforms in the adult caudal epididymis.

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“…Also, it seems that epidermal growth factor involves in regulation of Cx43 expression in human cauda epididymis (Dubé et al, 2012). Moreover, our recent research demonstrates disruption of expression of Cx isoforms in the adult IS by exposure to estradiol benzoate (EB), an estrogen agonist, and/or flutamide (Flu), an androgen antagonist, at 1 week of postnatal age (Lee, 2014). Expressional changes of some Cx isoforms are dose-dependent or steroidal hormonespecific but expression patterns of others are not (Lee, 2014).…”

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confidence: 99%

Exogenous Exposure to Estradiol Benzoate or Flutamide at the Weaning Age Alters Expression of Connexin Isoforms in the Initial Segment of Male Rat

Lee¹

2015

Development &Amp; Reproduciton

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Connexin (Cx) is a complex which allows direct communication between neighboring cells via exchange of signaling molecules and eventually leads to functional harmony of cells in a tissue. The initial segment (IS) is an excurrent duct of male reproductive tract and expression of numerous genes in the IS are controlled by androgens and estrogens. The effects of these steroid hormones on gene expression in the IS during postnatal development have not extensively examined. The present research investigated expressional modulation of Cx isoforms in the IS by exogenous exposure to estrogen agonist, estradiol benzoate (EB), or androgen antagonist, flutamide (Flu), at weaning age. Two different doses of EB or Flu were subcutaneously administrated in 21-day old of male rats, and expressional changes of Cx isoforms in the adult IS were analyzed by quantitative real-time PCR. Treatment of a low-dose EB (0.015 μg/kg body weight) resulted in an increased expression of Cx31 gene and a decreased expression of Cx37 gene. A high-dose EB (1.5 μg/kg body weight) treatment caused an increase of Cx31 gene expression. Increased levels of Cx30.3 and Cx40 transcripts were observed with a low-dose Flu (500 μg/kg body weight) treatment. Treatment of high-dose Flu (50 mg/kg body weight) led to expressional increases of Cx30.3, 40, and 43 genes. Our previous and present findings suggest differential responsiveness on gene expression of Cx isoforms in the IS by androgens and estrogens at different postnatal ages.

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Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide

Cited by 7 publications

References 14 publications

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Effects of Exposure to Estradiol Benzoate or Flutamide at the Weaning Age on Expression of Connexins in the Caudal Epididymis of Adult Rat

Exogenous Exposure to Estradiol Benzoate or Flutamide at the Weaning Age Alters Expression of Connexin Isoforms in the Initial Segment of Male Rat

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