Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Minakuchi, Chieka; Ishii, Fumika; Washidu, Yumiko; Ichikawa, Akio; Tanaka, Toshiharu; Miura, Ken; Shinoda, Tetsuro

doi:10.1016/j.jinsphys.2015.04.008

Cited by 31 publications

(31 citation statements)

References 35 publications

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“…). In previous studies, the transcript of MFE genes showed a significantly higher level in the corpora allata (CA) in Schistocerca gregaria (Marchal et al ., ), Bombyx mori (Daimon et al ., ), Diploptera punctate (Helvig et al ., ) and Tribolium castaneum (Minakuchi et al ., ). According to the specific activity of the MFE regulating the synthesis of JH, the abundant transcripts of EcMFE in the head in the male and female blister beetles may be attributed to the synthesis of JH from methyl farnesoate occurring in the CA.…”

Section: Discussionmentioning

confidence: 97%

Characterized cantharidin distribution and related gene expression patterns in tissues of blister beetles, Epicauta chinensis

Jiang

et al. 2017

Insect Science

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Cantharidin (CTD), a terpenoid defensive toxin mainly produced by blister beetles, is widely known by its toxicity to both cancer cells and pests. However, little information is known about its biosynthesis in blister beetles. In this study, first we determined the CTD content in various tissues of adult blister beetles on different days after mating, and then detected the temporal and spatial expression patterns of genes related to CTD biosynthesis in Epicauta chinensis. Results revealed that the accessory gland is the source of the highest CTD production. The second highest level was in the fat body in male blister beetles after mating. In females, the highest CTD content was in the reproductive system except the ovary after mating. As revealed by messenger RNA expression level analysis, the highest levels of 3-hydroxy-3-methylglutary-CoA reductase (HMGR) and juvenile hormone epoxide hydrolase (JHEH) transcripts of E. chinensis were observed in the fat body in males after mating. However, the highest transcript level of EcHMGR was in the ovary and EcJHEH was maintained at a nearly similar level in females. The transcript level of methyl-farnesoate epoxide was significantly higher in the head and that of CYP4BM1 in the midgut in both male and female E. chinensis. We speculate that the fat body may play a more important role than other tissues on the CTD biosynthesis in male E. chinensis after mating. There may be multiple tissues involved in the process of CTD biosynthesis. These four genes probably play regulatory roles in different tissues in males.

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Section: Discussionmentioning

confidence: 97%

Characterized cantharidin distribution and related gene expression patterns in tissues of blister beetles, Epicauta chinensis

Jiang

et al. 2017

Insect Science

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“…JH is a developmental regulating hormone that plays a critical role in the regulation of insect metamorphosis, especially in larvae. The inconsistency of expression levels of EcMFE and EcJHAMT with cantharidin production in larvae may be due to the high synthesis of JH III in larvae of E. chinensis 24, 29 . Furthermore, similar phenomena were found in female adults EcMFE and EcJHAMT transcripts were more abundance in females after mating than males but EcJHEH expression levels were lower.…”

Section: Discussionmentioning

confidence: 99%

“…For EcMFE gene, the high expression levels exhibit in the head and reproductive system reveal that EcMFE transcript level may appear more relevant with the synthesis of JH III than cantharidin. Previous studies suggest that the synthesis of JH III is performed primarily by the epoxidation of FA into JH III acid before the esterification of JH III acid into JH III in Lepidoptera 29, 41 . In orthopteroid insects, esterification of FA into MF occurs before epoxidation of MF into JH III 42 .…”

Section: Discussionmentioning

confidence: 99%

“…Recently research has shown that JHAMT protein in T. castaneum can catalyze the methylation of both FA and JH III acid to generate MF and JH III, respectively 24 . And TcCYP15A1 the methyl farnesoate epoxidase in red flour beetle epoxidizes both FA and MF to generate JH III acid and JH III, respectively 29 . Therefore it appears that there are two pathways to synthesize JH III in beetles.…”

Section: Discussionmentioning

confidence: 99%

“…The activity of epoxidation FA to JH III acid was demonstrated in B. mori 28 . MFEs were also identified and characterized in other species, such as T. castaneum 29 and S. gregaria 25 . In insects, JH III is principally degraded by JH epoxide hydrolase (JHEH) hydrolyzing JH III to JH III diol or converting JH III to JH III acid diol via JH III acid with the presence of JH esterase 30–32 .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis

Jiang

Zhang

2017

Sci Rep

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Cantharidin is a defensive toxin biosynthesized by blister beetles. It is well known for medical applications and toxicity. However, the biosynthesis process of cantharidin is still not well understood. In the present study, three genes (methyl farnesoate epoxidase (EcMFE), juvenile hormone acid O-methyltransferase (EcJHAMT) and juvenile hormone epoxide hydrolase (EcJHEH)) were identified from Epicauta chinensis. The temporal and spatial expression patterns of these three genes revealed that the expression levels of EcMFE and EcJHEH were high in the first instar larval stage of E. chinensis with EcJHEH transcripts highest in the fifth larval instar. The expression level of EcJHAMT was significantly higher in the 2nd and 3rd larval instars. The transcripts of EcMFE, EcJHEH and EcJHAMT showed a similar tendency with the cantharidin production in male blister beetles after mating. We verified the functions of these three genes in cantharidin biosynthesis using the RNA interference method. Interference of EcMFE and EcJHEH significantly inhibited the biosynthesis of cantharidin in male E. chinensis after mating, but interference of EcJHAMT has no apparent influence on cantharidin biosynthesis. We propose that EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but JH III might not be the direct precursor of cantharidin.

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Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Wang

et al. 2022

Arch Insect Biochem Physiol

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Cytochrome P450 monooxygenases (CYPs) are present in almost all areas of the tree of life. As one of the largest and most diverse superfamilies of multifunctional enzymes, they play important roles in the metabolism of xenobiotics and biosynthesis of endogenous compounds, shaping the success of insects. In this study, the CYPome (an omics term for all the CYP genes in a genome) diversification was examined in the four Tenebrionidea species through genome-wide analysis. A total of 483 CYP genes were identified, of which 103, 157, 122, and 101 were respectively deciphered from the genomes of Tebebrio molitor, Asbolus verucosus, Hycleus cichorii and Hycleus phaleratus. These CYPs were classified into four major clans (mitochondrial, CYP2, CYP3, and CYP4), and clans CYP3 and CYP4 are most diverse. Phylogenetic analysis showed that most CYPs of these Tenebrionidea beetles from each clan had a very close 1:1 orthology to each other, suggesting that they originate closely and have evolutionally conserved function. Expression analysis at different developmental stages and in various tissues showed the life stage-, gut-, salivary gland-, fat body-, Malpighian tubule-, antennae-, ovary-and testis-specific expression patterns of T. molitor CYP genes, implying their various potential roles in development, detoxification, immune response, digestion, olfaction, and reproduction.Our studies provide a platform to understand the evolution

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Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Cited by 31 publications

References 35 publications

Characterized cantharidin distribution and related gene expression patterns in tissues of blister beetles, Epicauta chinensis

Characterized cantharidin distribution and related gene expression patterns in tissues of blister beetles, Epicauta chinensis

Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

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