Expression studies of neuronatin in prenatal and postnatal rat pituitary

Kanno, Noriatsu; Higuchi, Masashi; Yoshida, Saishu; Yako, Hideji; Chen, Mo; Ueharu, Hiroki; Nishimura, Naoto; Katō, Takako; Kato, Yukio

doi:10.1007/s00441-015-2325-2

Cited by 13 publications

(22 citation statements)

References 33 publications

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“…6 However, the role of NNAT in vivo is poorly understood. Recently, we found that NNAT first appears in and occupies stem/progenitor cells in the rat embryonic pituitary primordium and is then downregulated during differentiation into mature hormone-producing cells, suggesting that NNAT may be involved in pituitary development 7 Moreover, we found that NNAT localizes in various subcellular organelles in addition to the endoplasmic reticulum (ER), as reported previously. 8…”

Section: Introductionsupporting

confidence: 85%

“…In the pancreas, NNAT localizes in the ER of β-cells, 8 However, in a previous study of the pituitary gland, we observed, for the first time, that NNAT was widely localized in subcellular organelles in addition to the ER. 7 Consequently, we have re-examined subcellular localization of NNAT in the adult pancreas using an antibody against PDI, a marker of the ER (Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

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Characteristic Localization of Neuronatin in Rat Tissues

Kanno

Yoshida

Katō

et al. 2018

Preprint

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1 Neuronatin (Nnat) is expressed in the pituitary, pancreas, and other tissues; however, the 2 function of NNAT is still unclear. Recent studies have demonstrated that NNAT is 3 localized in the sex determining region Y-box 2-positive stem/progenitor cells in the 4 developing rat pituitary primordium and is downregulated during differentiation into 5 mature hormone-producing cells. Moreover, NNAT is widely localized in subcellular 6 organelles, excluding the Golgi. Here, we further evaluated NNAT expression and 7 intracellular localization in embryonic and postnatal rat tissues such as the pancreas, 8 tongue, whisker hair follicle, and testis. Immunohistochemistry showed that NNAT was 9 localized in undifferentiated cells (i.e., epithelial basal cells and basement cells in the 1 0 papillae of the tongue and round and elongated spermatids of the testis) as well as in 1 1 differentiated cells (insulin-positive cells and exocrine cells of the pancreas, taste 1 2 receptor cells of the fungiform papilla, the inner root sheath of whisker hair follicles, 1 3 and spermatozoa). Additionally, NNAT showed novel intracellular localization in 1 4 acrosomes in the spermatozoa. Because the endoplasmic reticulum (ER) is excluded 1 5from spermatozoa and sarco/ER Ca 2+ -ATPase isoform 2 (SERCA2) is absent from the 1 6 inner root sheath, these findings suggested that NNAT localization in the ER and its 1 7 interaction with SERCA2 were cell-or tissue-specific properties.

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Section: Introductionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Characteristic Localization of Neuronatin in Rat Tissues

Kanno

Yoshida

Katō

et al. 2018

Preprint

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“…For example, the NNAT product, neuronatin, is expressed in prenatal and postnatal rat pituitary [23]. However, the role of neuronatin in the adult pituitary has not been clarified yet.…”

Section: Discussionmentioning

confidence: 99%

Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation

Pandey

Mizukami

Watanabe

et al. 2017

The Journal of Veterinary Medical Science

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We aimed to determine gene expression patterns in the anterior pituitary (AP) of heifers before and after ovulation via deep sequencing of the transcriptome (RNA-seq) to identify new genes and clarify important pathways. Heifers were slaughtered on the estrus day (pre-ovulation; n=5) or 3 days after ovulation (post-ovulation; n=5) for AP collection. We randomly selected 4 pre-ovulation and 4 post-ovulation APs, and the ribosomal RNA-depleted poly (A)+RNA were prepared to assemble next-generation sequencing libraries. The bovine APs expressed 12,769 annotated genes at pre- or post-ovulation. The sum of the reads per kilobase of exon model per million mapped reads (RPKM) values of all transcriptomes were 599,676 ± 38,913 and 668,209 ± 23,690, and 32.2 ± 2.6% and 44.0 ± 4.4% of these corresponded to the AP hormones in the APs of pre- and post-ovulation heifers, respectively. The bovine AP showed differential expression of 396 genes (P<0.05) in the pre- and post-ovulation APs. The 396 genes included two G-protein-coupled receptor (GPCR) genes (GPR61 and GPR153) and those encoding 13 binding proteins. The AP also expressed 259 receptor and other 364 binding proteins. Moreover, ingenuity pathway analysis for the 396 genes revealed (P=2.4 × 10−3) a canonical pathway linking GPCR to cytoskeleton reorganization, actin polymerization, microtubule growth, and gene expression. Thus, the present study clarified the novel genes found to be differentially expressed before and after ovulation and clarified an important pathway in the AP.

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“…Other markers expressed in the SOX2 stem cell niche include Sox9, Oct4, Gfra2, Ret, Ly6a (Sca-1), Prop1, Prrx1, Cdh1 (E-cadherin), S100β , β-catenin, neuronatin, neurturin, and Cxadr (Coxsackievirus and adenovirus receptor) (3,73,75,76,79–81). Clonogenic potential has been demonstrated for cells expressing Sox2, Sox9, Gfra2 , and S100b , but the remaining markers have not been tested yet.…”

Section: Pituitary Stem Cells: Postnatal Pituitary Cell Turnover and mentioning

confidence: 99%

Regulation of pituitary stem cells by epithelial to mesenchymal transition events and signaling pathways

Cheung

Davis

Brinkmeier

et al. 2017

Molecular and Cellular Endocrinology

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The anterior pituitary gland is comprised of specialized cell-types that produce and secrete polypeptide hormones in response to hypothalamic input and feedback from target organs. These specialized cells arise from stem cells that express SOX2 and the pituitary transcription factor PROP1, which is necessary to establish the stem cell pool and promote an epithelial to mesenchymal-like transition, releasing progenitors from the niche. The adult anterior pituitary responds to physiological challenge by mobilizing the SOX2-expressing progenitor pool and producing additional hormone-producing cells. Knowledge of the role of signaling pathways and extracellular matrix components in these processes may lead to improvements in the efficiency of differentiation of embryonic stem cells or induced pluripotent stem cells into hormone producing cells in vitro. Advances in our basic understanding of pituitary stem cell regulation and differentiation may lead to improved diagnosis and treatment for patients with hypopituitarism.

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Expression studies of neuronatin in prenatal and postnatal rat pituitary

Cited by 13 publications

References 33 publications

Characteristic Localization of Neuronatin in Rat Tissues

Characteristic Localization of Neuronatin in Rat Tissues

Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation

Regulation of pituitary stem cells by epithelial to mesenchymal transition events and signaling pathways

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