Expression specificity of CBP1 is regulated by transcriptional repression during vegetative growth of Magnaporthe oryzae

Nakajima, Yuichi; Saitoh, K; Arie, Tsutomu; Teraoka, Tohru; Kamakura, Takashi

doi:10.2323/jgam.56.437

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Transformation of M. oryzae was performed as described previously. 26,27) To prepare protoplasts, mycelia were harvested and treated with 30 mg/mL of Lysing enzymes from Trichoderma harzianum (Sigma-Aldrich) and 10 mg/mL of Yatalase (Takara Bio, Kusatsu, Japan) solution in osmotic medium (1.2 M MgSO 4 , 10mM sodium phosphate, pH 7.0) at 28 C overnight. Protoplasts were collected by centrifugation followed by overlaying of ST buffer (0.6 M sorbitol, and 100 mM Tris-HCl pH 7.5).…”

Section: Table 1 Primers Used In This Studymentioning

confidence: 99%

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

Ishii

Kumasaka

Nagashima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Roxithromycin (RXM), active against prokaryotes, has beneficial side effects such as anti-cancer activities on mammalian cells, but the mechanisms underlying these effects remain unclear. We found that RXM inhibited the cellular differentiation of the rice blast fungus Magnaporthe oryzae. Hence, we screened the targets of RXM by the T7 phage display method with fungal genomic DNA, and identified MoCDC27 (M. oryzae Cell Division Cycle 27) as a candidate. We generated mocdc27 knockdown mutants that the appressoria formation was less affected by RXM. A complemented mutant restored sensitivity against RXM to the level of the wild type. These results suggest that MoCDC27 was involved in the inhibition of appressorium formation by RXM, and that the complex of RXM-MoCDC27 affected another molecule involved in appressorium formation. The T7 phage display method with fungal genomic DNA can be a useful tool in the quest for drug target.

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Section: Table 1 Primers Used In This Studymentioning

confidence: 99%

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

Ishii

Kumasaka

Nagashima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Regulatory network of genes associated with stimuli sensing, signal transduction and physiological transformation of appressorium inMagnaporthe oryzae

et al. 2018

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Rice blast caused by Magnaporthe oryzae is the most destructive disease affecting the rice production (Oryza sativa), with an average global loss of 10–30% per annum. Recent reports have indicated that the fungus also inflicts blast disease on wheat (Triticum aestivum) posing a serious threat to the wheat production. Due to its easily detected infectious process and manoeuvrable genetic manipulation, M. oryzae is considered a model organism for exploring the molecular mechanism underlying fungal pathogenicity during the pathogen–host interaction. M. oryzae utilises an infectious structure called appressorium to breach the host surface by generating high turgor pressure. The appressorium development is induced by physical and chemical cues which are coordinated by the highly conserved cAMP/PKA, MAPK and calcium signalling cascades. Genes involved in the appressorium development have been identified and well studied in M. oryzae, a summary of the working gene network linking stimuli sensing and physiological transformation of appressorium is needed. This review provides a comprehensive discussion regarding the regulatory networks underlying appressorium development with particular emphasis on sensing of appressorium inducing stimuli, signal transduction, transcriptional regulation and the corresponding developmental and physiological responses. We also discussed the crosstalk and interaction of various pathways during the appressorium development.

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Key transcription factors required for outburst of rice blast disease in Magnaporthe oryzae

Wang,

Huang,

Khan

et al. 2024

Phytopathol Res

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Rice blast is a serious threat to the safe production of grain crops such as rice and wheat. Sporulation, appressorium formation, and invasive growth of Magnaporthe oryzae are the key stages of the development and spread of rice blast epidemics. M. oryzae is a hemibiotrophic fungus that undergoes changes in available carbon sources during the infection cycle. Lipid is a major storage for M. oryzae spores and a major carbon source used in glycerol synthesis and turgor pressure generation in appressoria. The formation of a dense cell wall melanin layer is necessary for an appressorium to produce turgor and to be pathogenic. The plant cell wall is an important carbon source during the infection stage of M. oryzae. Transcription factors regulate gene expression in fungi and are key intermediates between the reception of external environmental signals and the control of development and pathogenicity in M. oryzae. The disease cycle of M. oryzae is controlled by some key transcription factors, such as sporulation by Cos1 and Hox2, appressorium formation by Sfl1, Hox7, and Vrf1, invasive growth by Mst12 and Mig1, and resistance to host basal immunity by Ap1 and Atf1. This review focuses on describing the key transcription factors of M. oryzae that regulate sporulation, appressorium formation, invasive growth, lipid metabolism, carbohydrate metabolism, melanin synthesis, oxidative response, and host basal immunity, as well as the working mechanism of the transcription factors.

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Expression specificity of CBP1 is regulated by transcriptional repression during vegetative growth of Magnaporthe oryzae

Cited by 3 publications

References 23 publications

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

Regulatory network of genes associated with stimuli sensing, signal transduction and physiological transformation of appressorium inMagnaporthe oryzae

Key transcription factors required for outburst of rice blast disease in Magnaporthe oryzae

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