1991
DOI: 10.1073/pnas.88.16.7313
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Expression, secretion, and lipid-binding characterization of the N-terminal 17% of apolipoprotein B.

Abstract: The N-termnal 17% of human apolipoprotein B (apoB-17) was expressed in murine C127 cells following transfection with a bovine papilloma virus-based expression vector. A permanent cell line overexpressing the expected 89-kDa protein was selected and characterized. Pue-chase experiments showed that the depletion of intracellular apoB-17follows an apparent first-order kinetics with tl2 = 51 min.Under conditions of continuous labeling, >60% of the total synthesized apoB-17 was secreted in a soluble form, =98% lipi… Show more

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Cited by 58 publications
(122 citation statements)
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“…Third, Rava et al (60) demonstrated that MTP is not required for the secretion of apoB18. Relevant to this finding are studies demonstrating that apoB17 readily associates with phospholipids (61)(62)(63). Assuming that PL transfer activity of MTP is sufficient for the formation of primordial apoB particles, it is reasonable to expect that the secretion of apoB18 in COS cells would increase, at least partially, by the expression of Drosophila MTP; this was not observed by Rava et al (60).…”
Section: Discussionmentioning
confidence: 71%
“…Third, Rava et al (60) demonstrated that MTP is not required for the secretion of apoB18. Relevant to this finding are studies demonstrating that apoB17 readily associates with phospholipids (61)(62)(63). Assuming that PL transfer activity of MTP is sufficient for the formation of primordial apoB particles, it is reasonable to expect that the secretion of apoB18 in COS cells would increase, at least partially, by the expression of Drosophila MTP; this was not observed by Rava et al (60).…”
Section: Discussionmentioning
confidence: 71%
“…It has been proposed that this region has a strong affinity for phospholipids (28,29,31). In this study, we use a "divide and conquer" approach to map the phospholipid recruiting elements in the βα1 domain of apoB.…”
Section: Discussionmentioning
confidence: 99%
“…In fact, B17 binds both phospholipid vesicles and phospholipid-coated triglyceride emulsions in vitro (28)(29)(30). It also has the potential to convert multilamellar DMPC into smaller particles (29).…”
mentioning
confidence: 99%
“…Indeed, one of the hallmarks of the amino-terminal ␤␣ 1 domain of apoB (e.g. apoB17-20) is its capacity to interact spontaneously with lipid surfaces, including phospholipid vesicles (31,32), emulsion particles (33), oil droplets (34), and the inner leaflet of ERderived microsomal vesicles (35). Thus, the observation that the extension of apoB19.5 to apoB20.5 is associated with a simultaneous, discontinuous rise in the mean apoB residue area and fractional particle lipid volume (Fig.…”
Section: Resultsmentioning
confidence: 99%