Expression, purification and properties of lycopene cyclase from <i>Erwinia uredovora</i>

Schnurr, Georg; Misawa, Norihiko; Sandmann, Gerhard

doi:10.1042/bj3150869

Cited by 65 publications

(39 citation statements)

References 27 publications

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“…Similar to PDS and ZDS, other enzymes involved in carotenoid biosynthesis also contain a conserved FAD binding motif in their N termini, for example, CRTISO, LCY-E, LCY-B, and ZEP, suggesting the involvement of redox balance in these reactions even though they do not involve net electron transfer. This is the case in lycopene b-cyclase, which was active in vitro only in the presence of NADH or NADPH (Schnurr et al, 1996), and carotenoid isomerase, which was dependent on redox driving force (Isaacson et al, 2004). Involvement of FAD in ZEP activity has also been demonstrated (Buch et al, 1996).…”

Section: Involvement Of Orr In the Carotenoid Biosynthetic Pathwaymentioning

confidence: 99%

An Orange Ripening Mutant Links Plastid NAD(P)H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation

et al. 2010

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In higher plants, the plastidial NADH dehydrogenase (Ndh) complex supports nonphotochemical electron fluxes from stromal electron donors to plastoquinones. Ndh functions in chloroplasts are not clearly established; however, its activity was linked to the prevention of the overreduction of stroma, especially under stress conditions. Here, we show by the characterization of Orr Ds , a dominant transposon-tagged tomato (Solanum lycopersicum) mutant deficient in the NDH-M subunit, that this complex is also essential for the fruit ripening process. Alteration to the NDH complex in fruit changed the climacteric, ripening-associated metabolites and transcripts as well as fruit shelf life. Metabolic processes in chromoplasts of ripening tomato fruit were affected in Orr Ds , as mutant fruit were yellow-orange and accumulated substantially less total carotenoids, mainly b-carotene and lutein. The changes in carotenoids were largely influenced by environmental conditions and accompanied by modifications in levels of other fruit antioxidants, namely, flavonoids and tocopherols. In contrast with the pigmentation phenotype in mature mutant fruit, Orr Ds leaves and green fruits did not display a visible phenotype but exhibited reduced Ndh complex quantity and activity. This study therefore paves the way for further studies on the role of electron transport and redox reactions in the regulation of fruit ripening and its associated metabolism.

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Section: Involvement Of Orr In the Carotenoid Biosynthetic Pathwaymentioning

confidence: 99%

An Orange Ripening Mutant Links Plastid NAD(P)H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation

et al. 2010

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“…Lycopene cyclase (CrtY) from Erwiizia was expressed in E. coli, purified in an active form and assayed as described [21]. I n vitm assays were carried out by incubation of about 20 pg CrtY, a soybean lipid suspension containing 1.5 pg purified neurosporene and NADPH (final concentration 5 mM) in 0.5 ml SO mM Tridmalate, pH 6.7.…”

Section: Methodsmentioning

confidence: 99%

The Carotenoid 7, 8‐Dihydro‐ψ end Group can be Cyclized by the Lycopene Cyclases from the Bacterium Erwinia Uredovora and the Higher Plant Capsicum Annuum

Takaichi

Sandmann

Schnurr

et al. 1996

European Journal of Biochemistry

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The genes for geranylgeranyl diphosphate synthase (crtE) and phytoene synthase (crtB) from the epiphytic bacterium Erwinia uredovora and the phytoene desaturase gene from the photosynthetic bacterium Rhodobacter capsulatus (Rc‐crtI) were introduced into Escherichia coli, which resulted in the accumulation of the acyclic carotenoid, neurosporene. Further introduction of the lycopene cyclase gene from E. uredovora (crtY) or the higher plant Capsicum annuum (Icy) resulted in the production of a bicyclic carotenoid, 7,8‐dihydro‐β‐carotene, via monocyclic β‐zeacarotene. ζ‐Carotene was also found to be cyclized to bicyclic 7,8,7′,8′‐tetrahydro‐β‐carotene by the Erwinia cyclase. These results indicate that both lycopene cyclases can cyclize a 7,8‐dihydro‐ψ end group to a 7,8‐dihydro‐β end group, in addition to the usual cyclization of the ψ end group to the β end group. Furthermore, β‐carotene hydroxylase from Enwnia (CrtZ) was able to add a hydroxyl group to the 7,8‐dihydro‐β end group and the β end group.

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“…It was determined that the bacterial-type lycopene cyclase, CRTY, converts all-trans-lycopene to ␤-carotene and does not accept tetra-cis-prolycopene as a substrate (Schnurr et al, 1996). Likewise, it was shown that the plant-type lycopene cyclases efficiently convert all-trans-lycopene to ␤-carotene or ␦-carotene (Cunningham et al, 1993Pecker et al, 1996;Ronen et al, 1999).…”

Section: Requirement For Cis-trans Isomerization For Lycopene Cyclizamentioning

confidence: 99%

Cloning of tangerine from Tomato Reveals a Carotenoid Isomerase Essential for the Production of β-Carotene and Xanthophylls in Plants

et al. 2002

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Carotenoid biosynthesis in plants has been described at the molecular level for most of the biochemical steps in the pathway. However, the cis-trans isomerization of carotenoids, which is known to occur in vivo, has remained a mystery since its discovery five decades ago. To elucidate the molecular mechanism of carotenoid isomerization, we have taken a genetic map-based approach to clone the tangerine locus from tomato. Fruit of tangerine are orange and accumulate prolycopene (7Z,9Z,7 Z,9 Z-tetra-cis -lycopene) instead of the all-trans -lycopene, which normally is synthesized in the wild type. Our data indicate that the tangerine gene, designated CRTISO , encodes an authentic carotenoid isomerase that is required during carotenoid desaturation. CRTISO is a redox-type enzyme structurally related to the bacterial-type phytoene desaturase CRTI. Two alleles of tangerine have been investigated. In tangerine mic , loss of function is attributable to a deletion mutation in CRTISO , and in tangerine 3183 , expression of this gene is impaired. CRTISO from tomato is expressed in all green tissues but is upregulated during fruit ripening and in flowers. The function of carotene isomerase in plants presumably is to enable carotenoid biosynthesis to occur in the dark and in nonphotosynthetic tissues.

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Expression, purification and properties of lycopene cyclase from Erwinia uredovora

Cited by 65 publications

References 27 publications

An Orange Ripening Mutant Links Plastid NAD(P)H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation

An Orange Ripening Mutant Links Plastid NAD(P)H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation

The Carotenoid 7, 8‐Dihydro‐ψ end Group can be Cyclized by the Lycopene Cyclases from the Bacterium Erwinia Uredovora and the Higher Plant Capsicum Annuum

Cloning of tangerine from Tomato Reveals a Carotenoid Isomerase Essential for the Production of β-Carotene and Xanthophylls in Plants

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