Expression Profiling of Tumorigenesis Genes in Patients of Laryngeal Papillomatosis

Abstract

Recurrent Laryngeal papillomatosis (RLP) is a disease of the aero-digestive junction superimposed at the upper respiratory tract. Low risk human papillomavirus type 6 and 11 are infectious agents in RLP. Mainstay of treatment for RLP is CO2 laser vaporization for debulking of papilloma. Understanding of the gene changes at expression level might prove helpful for RLP management. The study was designed to evaluate the changes in tumorigenesis genes in RLP cases to mark the severity and aggression at gene expression level for disease management. Tissue samples and peripheral blood samples were acquired from clinically diagnosed RLP patients of age < 12 years undergoing CO2 laser vaporization. Control samples were taken from the normal thyroid tissue of individuals undergoing thyroidectomy. Relative quantitation of gene expression was done by real time polymerase reaction using cDNA samples. Up regulation of CXCL12/ CXCR4 signaling axis, parallel increase in GLUT-1 and over expression of IGF-I, EGFR and ER-α gene product was observed in RLP. On the other hand HIF-1a levels not discerned elevated along with VEGF, PIK3 and AKT gene expression profile. The expression analysis has provoked the possible strategies which can be planned to treat RLP by adjuvant and tailored medication to reduce multiple surgeries.