Expression profiling of transcription factors (TFs) in sugarcane X Colletotrichum falcatum interaction

Muthiah, Muthumeena; Ashwin, N. M. R.; Sundar, A. Ramesh; Malathi, P.; Viswanathan, R.

doi:10.1007/s13562-012-0157-7

Cited by 19 publications

(6 citation statements)

References 31 publications

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“…Having a good knowledge of the molecular mechanisms related to drought-responses and the relationship between water-utilized and carbon-fixed in plants would greatly help to increase yield 37 , and even breeding. A number of novel drought- or salt-related genes/microRNAs have been identified in sugarcane 5 6 7 35 36 37 38 . Though Iskandar et al 29 had identified GAPDH as a reliable reference for normalizing the transcriptional profile of target genes in two sugarcane cultivars and three Saccharum species and Ling et al 30 had identified GAPDH , eEF-1a and eIF-4a as the most stable and suitable references genes for normalizing target gene related to various experimental samples, several genes, such as β-actin , tubulin , 25S rRNA , GAPDH and poly- ubiquitin , were still frequently used as the reference genes for the normalization of gene expression in sugarcane.…”

Section: Discussionmentioning

confidence: 99%

The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses

Guo

Ling

et al. 2014

Sci Rep

110

106

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Sugarcane (Saccharum spp. hybrids) is a world-wide cash crop for sugar and biofuel in tropical and subtropical regions and suffers serious losses in cane yield and sugar content under salinity and drought stresses. Although real-time quantitative PCR has a numerous advantage in the expression quantification of stress-related genes for the elaboration of the corresponding molecular mechanism in sugarcane, the variation happened across the process of gene expression quantification should be normalized and monitored by introducing one or several reference genes. To validate suitable reference genes or gene sets for sugarcane gene expression normalization, 13 candidate reference genes have been tested across 12 NaCl- and PEG-treated sugarcane samples for four sugarcane genotypes using four commonly used systematic statistical algorithms termed geNorm, BestKeeper, NormFinder and the deltaCt method. The results demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and eukaryotic elongation factor 1-alpha (eEF-1a) were identified as suitable reference genes for gene expression normalization under salinity/drought-treatment in sugarcane. Moreover, the expression analyses of SuSK and 6PGDH further validated that a combination of clathrin adaptor complex (CAC) and cullin (CUL) as reference should be better for gene expression normalization. These results can facilitate the future research on gene expression in sugarcane under salinity and drought stresses.

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Section: Discussionmentioning

confidence: 99%

The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses

Guo

Ling

et al. 2014

Sci Rep

110

106

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“…In rice, all OsTLPs might involve in plant-pathogen interaction [ 12 ], besides, OsTLP2 can bind to the promoter of OsWRKY13 , which encodes an activator important in rice resistance to bacterial infection [ 13 ], suggesting that OsTLP2 might function as a transcription factor to regulate biotic stress tolerance. In coffee and sugarcane TLPs were provided that play a role in response to biotic stress [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

Wang

et al. 2021

BMC Plant Biol

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Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.

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“…Early induction of TFs (NAC, WRKY, MYB, TLP, and bZIP) can be actively involved or coordinate the defence against pathogen attack [133] Colletotrichum falcatum Red rot Clusters of ESTs expressed in resistant cultivars of sugarcane against red rot [134]…”

Section: Red Rotmentioning

confidence: 99%

Modern Biotechnologies: Innovative and Sustainable Approaches for the Improvement of Sugarcane Tolerance to Environmental Stresses

et al. 2021

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Sugarcane (Saccharum spp.) is one of the most important industrial cash crops, contributing to the world sugar industry and biofuel production. It has been cultivated and improved from prehistoric times through natural selection and conventional breeding and, more recently, using the modern tools of genetic engineering and biotechnology. However, the heterogenicity, complex poly-aneuploid genome and susceptibility of sugarcane to different biotic and abiotic stresses represent impediments that require us to pay greater attention to the improvement of the sugarcane crop. Compared to traditional breeding, recent advances in breeding technologies (molecular marker-assisted breeding, sugarcane transformation, genome-editing and multiple omics technologies) can potentially improve sugarcane, especially against environmental stressors. This article will focus on efficient modern breeding technologies, which provide crucial clues for the engineering of sugarcane cultivars resistant to environmental stresses.

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Expression profiling of transcription factors (TFs) in sugarcane X Colletotrichum falcatum interaction

Cited by 19 publications

References 31 publications

The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses

The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses

A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

Modern Biotechnologies: Innovative and Sustainable Approaches for the Improvement of Sugarcane Tolerance to Environmental Stresses

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