Expression Profiling of Nuclear Receptors in Human and Mouse Embryonic Stem Cells

Xie, CQ; Jeong, Y; Fu, M; Bookout, AL; Garcia-Barrio, MT; Sun, T; Bh, Kim; Xie, Y; Root, S; Zhang, J; Xu, Ruwei; Ye, Chen; Mangelsdorf, DJ

doi:10.1621/datasets.05005

Cited by 15 publications

(19 citation statements)

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“…As mentioned earlier, RA treatment induces neural differentiation of ES cells, which further promotes neuronal maturation but suppresses neuronal cell proliferation (Kim M et al, 2009). A recent finding suggests a potential molecular mechanism of RA action by which the expression of a subset of microRNAs (e.g., miR-134, miR-296, and miR-470) is induced by treatment with a vitamin A derivative.…”

Section: Embryonic Stem Cellsmentioning

confidence: 86%

“…Lineage-specific progenitors are mostly tissue-specific stem cells that include mesenchymal stem cells (MSCs) for adipose, muscle, or bone; hematopoietic stem cells (HSCs) for blood or bone remodeling; and neural stem cells (NSCs) for the nervous system. Since ethical concerns limit the therapeutic applicability of ES cells in humans, autograft therapy using self-stem cells has been pursued to treat diverse categories of diseases including neurologic abbreviation abbreviation AR NR3C4 Adipogenesis (Singh et al, 2006;Gupta V et al, 2008) Myogenesis (Singh et al, 2003(Singh et al, , 2009) COUP-TFβ NR2F2 Adipogenesis (Xu et al, 2008;Li et al, 2009) Myogenesis (Bailey et al, 1998Lee et al, 2004) DAX-1 NR0B1 ES cell stemness (Wang et al, 2006;Kim et al, 2008;Sun et al, 2008;Xie et al, 2009) ERβ NR3A2 NSC differentiation (Wang et al, 2001;Brannvall et al, 2002;Kishi et al, 2005) ERRβ NR3B2 ES cell stemness (Zhou et al, 2007;van den Berg et al, 2008;Zhang X et al, 2008) Induce iPS cells (Gu et al, 2005a;Feng et al, 2009) GCNF NR6A1 ES cell differentiation (Gu et al, 2005b) Neuronal differentiation (Sattler et al, 2004;Chung et al, 2006;Akamatsu et al, 2009 (Wallen-Mackenzie et al, 2003;Perlmann and Wallen-Mackenzie, 2004;Blin et al, 2008;Jacobs et al, 2009) PPARδ NR1C2 Transdifferentiation (Holst et al, 2003Wang et al, 2004;Teunissen et al, 2007) PPARγ NR1C3 Adipogenesis (Tontonoz et al, 1994;Hu et al, 1995;Fu et al, 2005;Crossno et al, 2006;Yu et al, 2006;...…”

Section: Introductionmentioning

confidence: 99%

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Nuclear receptor regulation of stemness and stem cell differentiation

Jeong

Mangelsdorf

2009

Exp Mol Med

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Stem cells include a diverse number of toti-, pluri-, and multi-potent cells that play important roles in cellular genesis and differentiation, tissue development, and organogenesis. Genetic regulation involving various transcription factors results in the self-renewal and differentiation properties of stem cells. The nuclear receptor (NR) superfamily is composed of 48 ligand-activated transcription factors involved in diverse physiological functions such as metabolism, development, and reproduction. Increasing evidence shows that certain NRs function in regulating stemness or differentiation of embryonic stem (ES) cells and tissue-specific adult stem cells. Here, we review the role of the NR superfamily in various aspects of stem cell biology, including their regulation of stemness, forward-and trans-differentiation events; reprogramming of terminally differentiated cells; and interspecies differences. These studies provide insights into the therapeutic potential of the NR superfamily in stem cell therapy and in treating stem cell-associated diseases (e.g., cancer stem cell).

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Section: Embryonic Stem Cellsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Nuclear receptor regulation of stemness and stem cell differentiation

Jeong

Mangelsdorf

2009

Exp Mol Med

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“…Because the VDR is known to be expressed in embryonic stem cells (56), the induction of miR-498 by 1,25(OH) 2 D 3 in embryonic stems cells appears possible, which may have profound implications in aging and lifespan regulation. Interestingly, miR-498 belongs to a cluster of primate-specific miRNA molecules absent in mice and rats (57), and the miR-498 target sequence in hTERT also appears missing in the 3Ј-UTR of mouse TERT.…”

Section: Discussionmentioning

confidence: 99%

1,25-Dihydroxyvitamin D3 Suppresses Telomerase Expression and Human Cancer Growth through MicroRNA-498

Kasiappan

Shen

Tse

et al. 2012

Journal of Biological Chemistry

113

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Background:Telomerase is essential for cancer cell growth. Results: MiR-498 is a novel 1,25(OH) 2 D 3 target gene that decreases telomerase, induces cell death, and suppresses tumor growth. Conclusion: MiR-498 is an important mediator of the anti-tumor activity of 1,25(OH) 2 D 3 . Significance: The studies define a new mechanism of telomerase regulation by small non-coding RNAs in response to 1,25(OH) 2 D 3 .

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“…cDNA was synthesized and subjected to polymerase chain reaction (PCR) amplification with primers specific for mouse SMC genes and other control genes with 18S RNA as an internal standard. PCR primers and reaction conditions are described in Supplementary Table S1 (Supplementary Materials available online at www.liebertonline.com=scd) [19].…”

Section: Quantitative Real Time-polymerase Chain Reactionmentioning

confidence: 99%

MicroRNA-1 Regulates Smooth Muscle Cell Differentiation by Repressing Kruppel-Like Factor 4

Xie

Huang

Sun

et al. 2011

Stem Cells and Development

140

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The role of microRNA-1 (miR-1) has been studied in cardiac and skeletal muscle differentiation. However, it remains unexplored in vascular smooth muscle cells (SMCs) differentiation. The aim of this study was to uncover novel targets of and shed light on the function of miR-1 in the context of embryonic stem cell (ESC) differentiation of SMCs in vitro. miR-1 expression is steadily increased during differentiation of mouse ESC to SMCs. Loss-of-function approaches using miR-1 inhibitors uncovered that miR-1 is required for SMC lineage differentiation in ESC-derived SMC cultures, as evidenced by downregulation of SMC-specific markers and decrease of derived SMC population. In addition, bioinformatics analysis unveiled a miR-1 binding site on the Kruppel-like factor 4 (KLF4) 3' untranslated region (3 0 UTR), in a region that is highly conserved across species. Consistently, miR-1 mimic reduced KLF4 3 0 UTR luciferase activity, which can be rescued by mutating the miR-1 binding site on the KLF4 3 0 UTR in the reporter construct. Additionally, repression of the miR-1 expression by miR-1 inhibitor can reverse KLF4 downregulation during ESC-SMC differentiation, which subsequently inhibits SMC differentiation. We conclude that miR-1 plays a critical role in the determination of SMC fate during retinoid acidinduced ESC=SMC differentiation, which may indicate that miR-1 has a role to promote SMC differentiation.

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Expression Profiling of Nuclear Receptors in Human and Mouse Embryonic Stem Cells

Cited by 15 publications

References 0 publications

Nuclear receptor regulation of stemness and stem cell differentiation

Nuclear receptor regulation of stemness and stem cell differentiation

1,25-Dihydroxyvitamin D3 Suppresses Telomerase Expression and Human Cancer Growth through MicroRNA-498

MicroRNA-1 Regulates Smooth Muscle Cell Differentiation by Repressing Kruppel-Like Factor 4

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