Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray

Larkin, Patrick; Folmar, Leroy C.; Hemmer, Michael J.; Poston, Arianna J.; Denslow, Nancy D.

doi:10.1289/txg.5752

Cited by 14 publications

(11 citation statements)

References 45 publications

(67 reference statements)

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“…Considering the basic criterion that a 2-fold change in transcript abundance represents differential expression [43] , [44] , the gene expression levels in survivors were not significantly different from controls (i.e. relative normalized gene expression levels in the range 0.5–2, see table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Differential Cytokine Gene Expression According to Outcome in a Hamster Model of Leptospirosis

Vernel-Pauillac

Goarant²

2010

PLoS Negl Trop Dis

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BackgroundParameters predicting the evolution of leptospirosis would be useful for clinicians, as well as to better understand severe leptospirosis, but are scarce and rarely validated. Because severe leptospirosis includes septic shock, similarities with predictors evidenced for sepsis and septic shock were studied in a hamster model.Methodology/Principal FindingsUsing an LD50 model of leptospirosis in hamsters, we first determined that 3 days post-infection was a time-point that allowed studying the regulation of immune gene expression and represented the onset of the clinical signs of the disease. In the absence of tools to assess serum concentrations of immune effectors in hamsters, we determined mRNA levels of various immune genes, especially cytokines, together with leptospiraemia at this particular time-point. We found differential expression of both pro- and anti-inflammatory mediators, with significantly higher expression levels of tumor necrosis factor α, interleukin 1α, cyclo-oxygenase 2 and interleukin 10 genes in nonsurvivors compared to survivors. Higher leptospiraemia was also observed in nonsurvivors. Lastly, we demonstrated the relevance of these results by comparing their respective expression levels using a LD100 model or an isogenic high-passage nonvirulent variant.Conclusions/SignificanceUp-regulated gene expression of both pro- and anti-inflammatory immune effectors in hamsters with fatal outcome in an LD50 model of leptospirosis, together with a higher Leptospira burden, suggest that these gene expression levels could be predictors of adverse outcome in leptospirosis.

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Section: Resultsmentioning

confidence: 99%

Differential Cytokine Gene Expression According to Outcome in a Hamster Model of Leptospirosis

Vernel-Pauillac

Goarant²

2010

PLoS Negl Trop Dis

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“…Ten genes cloned and sequenced from hepatopancreatic tissue of wild-caught blue crabs (metallothionein [two isoforms], MnSOD [two isoforms], Heat Shock Protein 70, hemocyanin, actin and ribosomal proteins S15, S20 and L23) were PCR amplified, spotted in duplicate onto neutral nylon macroarrays and hybridized as previously described (Larkin, Folmar, Hemmer, Poston, & Denslow, 2003). For each cDNA clone, the general background of each membrane was subtracted from the average value of the duplicate spots on the membrane.…”

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confidence: 99%

Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus

Brouwer

Larkin

Brown‐Peterson

et al. 2004

Marine Environmental Research

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“…Microarrays have been used to detect changes in gene transcription since the late 1990s (Schena et al, 1995). The use of microarrays to assess gene expression in sentinel organisms from an ecotoxicological standpoint has become widespread (Lettieri, 2006), and taxa for which they have been developed for this purpose include zebrafish (Danio rerio) (Handley-Goldstone et al, 2005), sheepshead minnow (Cyprinodon variegatus) (Larkin et al, 2003), fathead minnow (Pimephales promelas) (Larkin et al, 2007), European flounder (Platichthys flesus) (Williams et al, 2003), salmonids (Rise et al, 2004) and largemouth bass (Micropterus salmoides) (Larkin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Although the ability to evaluate the near-whole transcriptome response by fish to environmental contaminant exposure is now possible for numerous species, data regarding these responses to several chemicals are not readily available. Larkin et al (2002Larkin et al ( , 2003Larkin et al ( , 2007, Wang et al (2008), and Cohen et al (2008) have addressed the effects of endocrine disrupting compounds (e.g. 17 b-estradiol, 17 a-ethinyl estradiol, diethylstillbestrol, methoxychlor) on the transcriptome of largemouth bass, sheepshead minnow, fathead minnow and zebrafish.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants

Sanchez

Carter

Hammers

et al. 2011

J of Applied Toxicology

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Microarrays enable gene transcript expression changes in near-whole genomes to be assessed in response to environmental stimuli. We utilized oligonucleotide microarrays and subsequent gene set enrichment analysis (GSEA) to assess patterns of gene expression changes in male largemouth bass (Micropterus salmoides) hepatic tissues after a 96 h exposure to common environmental contaminants. Fish were exposed to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene via intraperitoneal injection with target body burdens of 3.0, 0.00067, 2.5, 50 and 100 µg g(-1), respectively. This was conducted in an effort to identify potential biomarkers of exposure. The expressions of 4, 126, 118, 137 and 58 mRNA transcripts were significantly (P ≤ 0.001, fold change ≥2×) affected by exposure to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene exposures, respectively. GSEA revealed that none, four, five, five and three biological function gene ontology categories were significantly influenced by exposure to these chemicals, respectively. We observed that cadmium chloride elicited ethanol metabolism responses, and along with PCB 126 and phenanthrene affected transcripts associated with protein biosynthesis. PCB 126, phenanthrene and toxaphene also influenced one-carbon compound metabolism while PCB 126 and phenanthrene affected mRNA transcription and mRNA export from the nucleus and may have induced an antiestrogenic response. Atrazine was found to alter the expression of few hepatic transcripts. This work has highlighted several biological processes of interest that may be helpful in the development of gene transcript biomarkers of chemical exposure in fish.

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Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray

Cited by 14 publications

References 45 publications

Differential Cytokine Gene Expression According to Outcome in a Hamster Model of Leptospirosis

Differential Cytokine Gene Expression According to Outcome in a Hamster Model of Leptospirosis

Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus

Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants

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