Expression profiling of DNA repair genes in human oocytes and blastocysts using microarrays

Jaroudi, S.; Kakourou, Georgia; Cawood, Suzanne; Doshi, Alpesh; Ranieri, Domenico Massimo; Serhal, Paul; Harper, Joyce; SenGupta, Sioban

doi:10.1093/humrep/dep224

Cited by 108 publications

(86 citation statements)

References 39 publications

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“…As has been demonstrated in human and mouse, zygotic DNA repair initially depends on the oocyte-born mRNAs and proteins and, additionally, on genes expressed very early in development (Derijck et al 2008, Jaroudi et al 2009). Therefore, the combined effect of sperm DNA damage and the capacity of the oocyte to repair it will determine the final impact on the offspring development (GonzalezMarin et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

González-Rojo¹,

Lombó²,

Herráez³

2016

Reproduction

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Section: Discussionmentioning

confidence: 99%

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

González-Rojo¹,

Lombó²,

Herráez³

2016

Reproduction

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“…Thus, transmutation of paternal genome may compromise embryo quality, viability and maintaining pregnancy, ultimately leading to recurrent pregnancy loss. Oocytes are well equipped to handle DNA damage in sperm by different pathways like direct reversal of damage, singlestrand damage repair, base excision repair, nucleotide excision repair, mismatch repair that repair sperm DNA damages [26,31]. Oocytes can repair sperm DNA damage, but there is a threshold beyond which sperm DNA may not be repaired, and accumulation of ethenonucleosides (type of DNA lesion) in sperm may inhibit nucleotide excision repair mechanism.…”

Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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“…An expression profile of DNA repair genes in human oocytes and blastocysts was previously established using microarrays [43,44]. This study was used as a guideline to identify mRNAs that were differentially expressed in human oocytes relative to blastocysts.…”

Section: Selection Of Mrna and Mirna For Analysismentioning

confidence: 99%

Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts

Tulay

Naja

Cascales-Roman

et al. 2015

J Assist Reprod Genet

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Purpose The aim of the study is to investigate the regulation of DNA repair genes by microRNAs (miRNAs). miRNAs are short non-coding RNAs that regulate transcriptional and post-transcriptional gene silencing. Several miRNAs that are expressed during preimplantation embryo development have been shown or are predicted to target genes that regulate cell cycle checkpoints and DNA repair in response to DNA damage. Methods This study compares the expression level of 20 miRNAs and 9 target transcripts involved in DNA repair. The statistical significance of differential miRNA expression between oocytes and blastocysts was determined by t test analysis using the GraphPad Prism v6 software. The possible regulatory roles of miRNAs on their target messenger RNAs (mRNAs) were analysed using a Pearson correlation test. Results This study shows for the first time that several miRNAs are expressed in human oocytes and blastocysts that target key genes involved in DNA repair and cell cycle checkpoints. Blastocysts exhibited statistically significant lower expression levels for the majority of miRNAs compared to oocytes (p<0.05). Correlation analyses showed that there was both inverse and direct association between miRNAs and their target mRNAs. Conclusions miRNAs target many mRNAs including ones involved in DNA repair mechanisms. This study suggests that miRNAs and their target mRNAs involved in DNA repair are expressed in preimplantation embryos. Similar to the miRNAs expressed in adult tissues, these miRNAs seem to have regulatory roles on their target DNA repair mRNAs during preimplantation embryo development.

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Expression profiling of DNA repair genes in human oocytes and blastocysts using microarrays

Cited by 108 publications

References 39 publications

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts

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