Expression Profiling of Clinical Specimens Supports the Existence of Neural Progenitor-Like Stem Cells in Basal Breast Cancers

Panaccione, Alex; Guo, Yan; Yarbrough, Wendell G.; Ivanov, Sergey V.

doi:10.1016/j.clbc.2017.01.007

Cited by 22 publications

(16 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies, the association between TRIM2 and osteosarcoma have not been reported. In studies focused on different types of carcinoma, TRIM2 has been detected to be significantly differentially expressed in ovarian cancer, breast cancer, cervical carcinoma, buccal mucosa carcinoma and follicular carcinoma (27)(28)(29)(30)(31)(32). In a study of ovarian cancer, the expression of TRIM2 was detected as upregulated, and negatively regulated Bim (7).…”

Section: Discussionmentioning

confidence: 99%

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma

Qin

Zhao

et al. 2018

Int J Oncol

View full text Add to dashboard Cite

The present study aimed to investigate candidate genes involved in the development and metastasis of osteosarcoma. Candidate genes were screened preliminarily from the Gene Expression Omnibus database and then validated using actual tumor tissues collected from patients with osteosarcoma. The cells were prepared and transfected with specific gene-targeted small interfering RNA followed by an MTS assay for cell viability detection and Transwell assays for cell migration and invasion capacity detection. The cell apoptosis was determined by flow cytometry and the protein level of the genes was detected by western blot analysis. An in vivo nude model was used and injected with cells to detect the functions of the genes. Transcriptome sequencing was performed to verify the regulation network, followed by reverse transcription-quantitative polymerase chain reaction and western blot analyses for validation. Increased tripartite motif-containing protein 2 (TRIM2) was detected in the osteosarcoma tumor tissues compared with normal tissues. The inhibition of TRIM2 induced lower cell viability and cell invasion capacity, and increased the rate of cell apoptosis. Decreased TRIM2 also inhibited the development and metastasis of osteosarcoma in the nude mouse models. The transcriptome sequencing revealed that the regulation of TRIM2 may be correlated with genes, Sirtuin 4, DNA damage inducible transcript 3, cAMP responsive element binding protein 5, G protein-coupled receptor 65 (GPR65) and ADP-ribosyltransferase 5. Western blot analysis indicated that TRIM2 regulated the development and metastasis of osteosarcoma via the phosphoinositide 3-kinase/protein kinase B signaling pathway. Therefore, TRIM2 performs important functions in regulating the development and metastasis of osteosarcoma.

show abstract

Section: Discussionmentioning

confidence: 99%

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma

Qin

Zhao

et al. 2018

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…Marked by expression of SOX10, similar CSC populations appear to drive breast basal-like carcinoma, melanoma, and other cancers that appear to arise from neural crest (14,50). We confirmed that ACC cultures maintained MYB fusions found in xenografts or primary tumors and identified a novel MYB fusion to a non-coding RNA.…”

Section: Discussionmentioning

confidence: 99%

MYB fusions and CD markers as tools for authentication and purification of cancer stem cells from salivary adenoid cystic carcinoma

et al. 2017

Self Cite

View full text Add to dashboard Cite

Cancer stem cells (CSC) are considered the major cause of aggressive tumor behavior, recurrence, metastases, and resistance to radiation, making them an attractive therapeutic target. However, isolation of CSC from tumor tissue and their characterization are challenging due to uncertainty about their molecular markers and conditions for their propagation. Adenoid cystic carcinoma (ACC), which arises predominantly in the salivary glands, is a slow-growing but relentless tumor that frequently invades nerves and metastasizes. New effective treatment approaches for ACC have not emerged over the last 40 years. Previously, based on a highly conserved SOX10 gene signature that we identified in the majority of ACC tumors, we suggested the existence in ACC of SOX10+ cells with neural stem properties and corroborated this hypothesis via isolation from ACC tissue a novel population of CSC, termed ACC-CSC. These cells co-expressed SOX10 and other ACC-intrinsic neural crest stem cell markers with CD133, a CSC cell surface marker, and activated NOTCH1 signaling suggesting that ACC is driven by a previously uncharacterized population of SOX10+/CD133+ cells with neural stem cell properties. Here, we authenticated ACC identity of our primary cultures by demonstrating that most of them harbor MYB-NFIB fusions, which are found in 86% of ACC. We demonstrated using CyTOF, a novel mass cytometry technology, that these cells express high β-catenin and STAT3 levels and are marked by CD24 and CD44. Finally, to streamline development of ACC cell lines, we developed RT-PCR tests for distinguishing mouse and human cells and used immunomagnetic cell sorting to eliminate mouse cells from long-term cell cultures. Overall, this study describes a new population of CSC that activates signaling pathways associated with poor prognosis, validates their ACC identity, and optimizes approaches that can be used for purification of ACC-CSC and generation of cell lines.

show abstract

“…Their results implied that basal breast cancer progression is stimulated by SOX10 + /CD133 + cells, which express neural stem cell-specific markers and have characteristics of cancer stem cells derived from the neural crest. Their results also provided clinical information for the development of cancer stem cell-targeted therapy ( Ivanov et al, 2013 ; Panaccione et al, 2016 , 2017 ). The expression of nestin mRNA is positively correlated with the expression of SOX10 mRNA in breast cancer.…”

Section: Roles Of Sox10 In Diverse Human Neoplasmsmentioning

confidence: 95%

“…SOX10 knockout reduces the rate of CD24 – /CD44 + cells and cancer stem cell properties, which ultimately inhibits tumor formation ( Feng et al, 2017 ). Panaccione et al (2016 , 2017) identified a highly conserved SOX10 /prominin-1 (CD133) gene marker in the clinical breast cancer data set. Their results implied that basal breast cancer progression is stimulated by SOX10 + /CD133 + cells, which express neural stem cell-specific markers and have characteristics of cancer stem cells derived from the neural crest.…”

Section: Roles Of Sox10 In Diverse Human Neoplasmsmentioning

confidence: 99%

Sex-Determining Region Y Chromosome-Related High-Mobility-Group Box 10 in Cancer: A Potential Therapeutic Target

Peng

Dong

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Sex-determining region Y-related high mobility group-box 10 (SOX10), a member of the SOX family, has recently been highlighted as an essential transcriptional factor involved in developmental biology. Recently, the functionality of SOX 10 has been increasingly revealed by researchers worldwide. It has been reported that SOX10 significantly regulates the proliferation, migration, and apoptosis of tumors and is closely associated with the progression of cancer. In this review, we first introduce the basic background of the SOX family and SOX10 and then discuss the pathophysiological roles of SOX10 in cancer. Besides, we enumerate the application of SOX10 in the pathological diagnosis and therapeutic potential of cancer. Eventually, we summarize the potential directions and perspectives of SOX10 in neoplastic theranostics. The information compiled herein may assist in additional studies and increase the potential of SOX10 as a therapeutic target for cancer.

show abstract

Expression Profiling of Clinical Specimens Supports the Existence of Neural Progenitor-Like Stem Cells in Basal Breast Cancers

Cited by 22 publications

References 81 publications

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma

MYB fusions and CD markers as tools for authentication and purification of cancer stem cells from salivary adenoid cystic carcinoma

Sex-Determining Region Y Chromosome-Related High-Mobility-Group Box 10 in Cancer: A Potential Therapeutic Target

Contact Info

Product

Resources

About