Expression profiling of CEACAM6 associated with the tumorigenesis and progression in gastric adenocarcinoma

Abstract

ABSTRACT. Carcinoembryonic antigen-related cellular adhesion molecule 6 (CEACAM6) is a member of the immunoglobulin superfamily and has been recently reported to affect the neoplastic, metastatic, and invasive ability of malignant cells by regulating intracellular signaling pathways during tumorigenesis and progression. We investigated the expression and amplification of CEACAM6 in relation to the clinicopathological and biological significance of gastric adenocarcinoma. Expression of CEACAM6 mRNA in 75 primary gastric adenocarcinom and 20 adjacent tissues compared to normal gastric mucosas were explored using real-time quantitative-polymerase chain reaction. Immunohistochemical assays were conducted to evaluate the expression and tissue distribution of CEACAM6 protein. Overexpression of CEACAM6 mRNA in both gastric adenocarcinoma (2.513 ± 0.869) and adjacent tissues (1.171 ± 0.428) was significantly higher than the relative expressions in non-neoplastic specimens (0.594 ± 0.513) (P < 0.01). CEACAM6 protein was present in 52 (69.33%) gastric adenocarcinomas, but Overexpression of CEACAM6 in gastric cancer not in normal gastric tissues. Adenocarcinomas with elevated CEACAM6 expression were significantly associated with lymph node metastases and advanced stages. There were no relationships between CEACAM6 expression and tumor size, histological differentiation, or different subtypes, respectively. Moreover, higher expression of CEACAM6 was found to be correlated with short postoperative survival time of patients with gastric cancer. Amplification and upregulation of CEACAM6 expression was observed in human gastric adenocarcinomas, which may be correlated with the generation or transformation of malignant cells, tumor aggressive progression, and clinical outcome. CEACAM6 may be a valuable biomarker screening for gastric tumor and novel predictor for patients in advanced stages of gastric cancer.

SHCBP1 is a novel target and exhibits tumor‑promoting effects in gastric cancer

“…Screening for new oncogenes and studying their mechanisms of action have important clinical diagnostic value for the early diagnosis of biomarkers and potential molecular therapeutic targets for GC. The metastasis of GC is a multi-step pathological process involving a loss of epithelial properties, such as reduced expression of E-cadherin, and a gain in mesenchymal phenotypes, such as enhanced expression of N-cadherin and vimentin (16). On one hand, tumor cells regulate the changes of their own molecules, so that they can develop in the direction of metastasis; on the other hand, they interact with other cells to form a special metastatic microenvironment and promote liver metastasis of GC (17).…”

Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 Promotes Carcinogenesis of Gastric Cancer and Anti-CEACAM6 Fluorescent Probe Can Diagnose the Precancerous Lesions

“…It was also reported that CEACAM6 expression was higher in pancreatic cancer with low differentiation than in medium differentiation, similarly, it was found that CEACAM6 expression was higher in tumor tissues than in adjacent normal tissues in colon cancer (31). An increasing number of studies have found that CEACAM6 plays an important role in GC progression and that it is a prognostic biomarker and potential therapeutic target for GC (32,33).…”

CEACAM6’s Role as a Chemoresistance and Prognostic Biomarker for Pancreatic Cancer: A Comparison of CEACAM6’s Diagnostic and Prognostic Capabilities with Those of CA19-9 and CEA

“…To our knowledge, our publication is the first report on CEACAM6 blood serum expression as a statistically significant PDAC prognostic biomarker. As CEACAM6 has universal oncogenic features, it has been reported as a relevant prognostic biomarker in other malignancies: cholangiocarcinoma, osteosarcoma, renal cancer, colorectal cancer, gastric cancer [39][40][41][42][43][44][45].…”