Expression profiling based on coexpressed modules in obese prepubertal children

Zhang, J. F.; Yao, Guijun; Wu, Yanyan

doi:10.4238/2012.august.31.5

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…Arguments have been presented that adipocyte stress which accumulates during fat loss, is a driving factor for fat regain during follow-up after return to energy balance [23]. This mechanical stress is concentrated at the focal adhesions linking the stress-fibres of the cytoplasm to the extracellular matrix [43][44][45][46]. In the present in-vitro study, we also observed a higher level of components of the focal adhesion when RF was compared to normal feeding.…”

Section: Discussionsupporting

confidence: 70%

Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain

et al. 2019

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Long-term weight loss maintenance is a problem of overweight and obesity. Changes of gene expression during weight loss (WL) by calorie restriction (CR) are linked to the risk of weight regain (WR). However, detailed information on genes/proteins involved in the mechanism is still lacking. Therefore, we developed an in-vitro model system for glucose restriction (GR) and refeeding (RF) to uncover proteome differences between GR with RF vs normal feeding, of which we explored the relation with WR after WL. Human Simpson-Golabi-Behmel Syndrome cells were subjected to changing levels of glucose to mimic the condition of CR and RF. Proteome profiling was performed by liquid chromatography tandem mass spectrometry. This in-vitro model revealed 44 proteins differentially expressed after GR and RF versus feeding including proteins of the focal adhesions. Four proteins showed a persistent up-or down-regulation: liver carboxylesterase (CES1), mitochondrial superoxide dismutase [Mn] (SOD2), alpha-crystallin B-chain (CRYAB), alpha-enolase (ENO1). In-vivo weight loss-induced RNA expression changes linked CES1, CRYAB and ENO1 to WR. Moreover, of these 44 proteins, CES1 and glucosidase II alpha subunit (GANAB) during follow up correlated with WR. Correlation clustering of in-vivo protein expression data indicated an interaction of these proteins with structural components of the focal adhesions and cytoplasmic filaments in the adipocytes.

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Section: Discussionsupporting

confidence: 70%

Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain

et al. 2019

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“…Individuals with increased body mass index (BMI) have been reported to display different gene expression patterns relative to those of normal BMI. In a study of omental adipose tissue from five obese and six non-obese pre-pubescent children, 342 differentially expressed genes were found between groups 20 , 21 . Similarly, extensive weight loss after bariatric surgery leads to significant increased expression in adipose tissue of several genes involved in lipid and mitochondrial metabolism 22 .…”

Section: Introductionmentioning

confidence: 99%

Development of multivariable models to predict change in Body Mass Index within a clinical trial population of psychotic individuals

Harrison

Gaughran

Murray

et al. 2017

Sci Rep

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Many antipsychotics promote weight gain, which can lead to non-compliance and relapse of psychosis. By developing models that accurately identify individuals at greater risk of weight gain, clinicians can make informed treatment decisions and target intervention measures. We examined clinical, genetic and expression data for 284 individuals with psychosis derived from a previously published randomised controlled trial (IMPACT). These data were used to develop regression and classification models predicting change in Body Mass Index (BMI) over one year. Clinical predictors included demographics, anthropometrics, cardiac and blood measures, diet and exercise, physical and mental health, medication and BMI outcome measures. We included genetic polygenic risk scores (PRS) for schizophrenia, bipolar disorder, BMI, waist-hip-ratio, insulin resistance and height, as well as gene co-expression modules generated by Weighted Gene Co-expression Network Analysis (WGCNA). The best performing predictive models for BMI and BMI gain after one year used clinical data only, which suggests expression and genetic data do not improve prediction in this cohort.

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“…This finding stresses the concept of concomitant growth of adipose tissue and its vasculature. In line with this, VWF is elevated in the plasma of obese children, and there is evidence for a differential expression in microarray studies [32,33,34,35].…”

Section: Discussionmentioning

confidence: 80%

Age- and BMI-Associated Expression of Angiogenic Factors in White Adipose Tissue of Children

Gaebler¹,

Haggenmüller²,

Kapapa³

et al. 2019

IJMS

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The growth of adipose tissue and its vasculature are tightly associated. Angiogenic factors have been linked to obesity, yet little is known about their expression during early childhood. To identify associations of angiogenic factors with characteristics on individual and tissue level, subcutaneous white adipose tissue samples were taken from 45 children aged 0–9 years undergoing elective surgery. We measured the expression of vascular endothelial growth factor A (VEFGA), fibroblast growth factor 1 and 2 (FGF1, FGF2), angiopoietin 1 and 2 (ANGPT1, ANGPT2), TEK receptor tyrosine kinase (TEK), and von Willebrand factor (VWF). In addition, we determined the mean adipocyte size in histologic tissue sections. We found positive correlations of age with FGF1 and FGF2 and a negative correlation with ANGPT2, with pronounced differences in the first two years of life. FGF1, FGF2, and ANGPT1 correlated positively with adipocyte size. Furthermore, we identified a correlation of ANGPT1 and TEK with body mass index-standard deviation score (BMI-SDS), a measure to define childhood obesity. Except for ANGPT2, all angiogenic factors correlated positively with the endothelial marker VWF. In sum, our findings suggest that differences related to BMI-SDS begin early in childhood, and the analyzed angiogenic factors possess distinct roles in adipose tissue biology.

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Expression profiling based on coexpressed modules in obese prepubertal children

Cited by 5 publications

References 36 publications

Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain

Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain

Development of multivariable models to predict change in Body Mass Index within a clinical trial population of psychotic individuals

Age- and BMI-Associated Expression of Angiogenic Factors in White Adipose Tissue of Children

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