Expression profiles of toll like receptors, MHC and cytokine genes along with viral load in organs of ducklings infected with an Indian isolate of duck enteritis virus

KUMAR, JYOTI; Dandapat, S.; Panickan, Sivasankar; Kumar, Ajay; Singh, Mithilesh; Bindu, Suresh; Dhama, Kuldeep

doi:10.1016/j.micpath.2022.105502

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Our results suggested that the liver and trachea had the highest viral load, while the heart had the lowest viral load. On the contrary, a previous study by Kumar et al showed that the spleen and liver had the highest viral loads [ 30 ]. This variation may be caused by the fact that they did not check the viral load in the trachea.…”

Section: Discussionmentioning

confidence: 87%

Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021

Liang

Guo

Yuan

et al. 2022

Animals

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Duck plague (DP) is a highly contagious viral disease in ducks caused by the duck plague virus (DPV). The DPV, a member of Herpesviridae, poses a severe threat to the waterfowl farming industry worldwide. In this study, we reported a recent outbreak of DPV in domestic laying ducks at 310 days of age from southern China in December 2021. The gross lesion, histopathologic examination, molecular detection, and genetic characterization studies of DPV are described here. As a result, gross lesions such as an enlarged congestive spleen and liver were observed. Liver with vacuolar degeneration and small vacuoles and spleen with hemosiderosis were remarkable microscopic findings. Our results suggested that the liver had the highest viral load, followed by the trachea, pancreas, kidney, brain, spleen, and heart. In addition, DPV was successfully isolated in chicken embryo fibroblast cell culture and designated as DP-GD-305-21. The UL2, UL12, UL41, UL47, and LORF11 genes of DP-GD-305-21 shared a high nucleotide homology with the Chinese virulent (CHv) strain and the Chinese variant (CV) strain. In conclusion, this study reports the isolation and molecular characterization of DPV from a recent outbreak in southern China. Our results contributed to the understanding of the pathological and molecular characterization of currently circulating DPV in China.

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Section: Discussionmentioning

confidence: 87%

Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021

Liang

Guo

Yuan

et al. 2022

Animals

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“…During DEV infection, upregulation of the expression of proinflammatory cytokines (IFN-α, IFN- β, IL-1β, IL-6) [ 10 , 11 , 12 ], RIG-I [ 12 ] and TLRs (TLR-2, 3, 21) [ 10 ] have been reported. However, the process by which innate immune response is regulated and the immune molecules involved in immune heamostais during DEV infection have not been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

TBK1 Mediates Innate Antiviral Immune Response against Duck Enteritis Virus

Wang

Hong

Werid

et al. 2022

Viruses

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Duck enteritis virus (DEV) can infect several types of waterfowl can cause high mortality and huge economic losses to the global waterfowl industry. Type I interferons (IFN) are important for host defense against virus infection through induction of antiviral effector molecules. TANK-binding kinase 1 (TBK1) is a key kinase required for the induction of type I IFNs; however, the role of TBK1 on DEV infection remains unclear. Here, we observed that the expression levels of TBK1 and IFN-β were upregulated during DEV infection in vivo and in vitro. Thus, the function of TBK1 on DEV infection was determined. The results showed that overexpression of TBK1 reduced DEV infection and knockdown of TBK1 resulted in the increased of DEV infection. Additionally, TBK1 overexpression upregulated the expression of IFN-β and a few interferon-stimulated genes (ISGs), which thus inhibited the synthesis of DEV glycoprotein B. On the other hand, the TBK1 inhibitor Amlexanox down-regulated the expression levels of IFN-β and IRF3. Interestingly, the expression levels of MAVS and GSK-3β were decreased in the cells treated with Amlexanox. Furthermore, overexpression of TBK1 activated the expression of upstream molecules MAVS and GSK-3β. Whereas, the expression of TBK1, IRF3 and IFN-β was inhibited by the GSK-3β inhibitor SB216763. Our findings suggest that DEV–stimulated TBK1 may be involved in defense against DEV infection.

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RIG-I expression pattern and cytokine profile in indigenous ducks infected with duck plague virus

Chabukdhara

Kalita

Tamuly

et al. 2023

Microbial Pathogenesis

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Expression profiles of toll like receptors, MHC and cytokine genes along with viral load in organs of ducklings infected with an Indian isolate of duck enteritis virus

Cited by 4 publications

References 46 publications

Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021

Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021

TBK1 Mediates Innate Antiviral Immune Response against Duck Enteritis Virus

RIG-I expression pattern and cytokine profile in indigenous ducks infected with duck plague virus

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