Expression profiles of select genes in cumulus–oocyte complexes from young and aged mares

Cox, Lindsay; Vanderwall, Dirk K.; Parkinson, Kate C.; Sweat, Alexis; Isom, S. Clay

doi:10.1071/rd14446

Cited by 15 publications

(10 citation statements)

References 60 publications

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“…In equine follicles and oocytes, gene expression, as studied by RT-qPCR, quantitatively and temporally differed with maternal age, suggesting a desynchronization of essential genes for maturation [51]. Moreover, a study on the expression of 48 genes in cumulusoocytes complexes from young (< 12 years of age) and old (> 18 years old) mares showed differential expression that may be related with the reduced developmental competence observed in old mares' oocytes [52]. Finally, genes related to mitochondrial replication and function were down-regulated in oocytes of old mares [16].…”

Section: Discussionmentioning

confidence: 96%

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Derisoud

Jouneau

Dubois³

et al. 2021

Preprint

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Background: Increased embryo loss as mares become older is a major consideration for breeders as older animals are currently used for reproduction in the equine industry. Lower embryo quality has been pointed out as partly responsible for this reduced fertility. Here, the effect of mare's age on blastocysts' gene expression was explored. Day 8 post ovulation embryos were collected by uterine flushing from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure (TE_part) or inner cell mass enriched (ICMandTE) trophoblast were obtained by embryo bisection and paired end, non-oriented RNA sequencing (Illumina, NextSeq500) was performed on each hemi-embryo. To discriminate gene expression in the ICM from that in the TE, deconvolution (DeMixT R package) was used on the ICMandTE dataset. Differential expression was analyzed (DESeq2) with embryo sex and diameter as cofactors using a false discovery rate < 0.05 cutoff. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results: Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity but modified gene expression in equine D8 embryos. The expression of genes in the ICM seemed to be more altered by maternal aging than in the TE. In both compartments, the expression of genes involved in mitochondrial function, translation and transcription due to chromatin modification were disturbed by maternal age. Mitosis, signaling and adhesion pathways and embryo development were particularly decreased in the ICM hemi-embryos from old mares. Finally, in TE, ion movement related genes were affected. Conclusions: This is the first study showing an effect of maternal age on gene expression in the equine blastocyst at Day 8 post ovulation. Maternal age, even for mares as young as 10 years old, disturbs mitosis, translation and transcription, cell signaling and adhesion as well as mitochondrial function and cell commitment of horse embryos. These perturbations may affect further embryo development and contribute to decreased fertility due to aging.

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Section: Discussionmentioning

confidence: 96%

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Derisoud

Jouneau

Dubois³

et al. 2021

Preprint

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“…ARL6IP6 is involved in DNA repair and apoptosis 38 , which is mainly expressed in nucleus and plasma membrane. Previous GWAS identi ed that ARL6IP6 is signi cantly associated with ischemic attacks 39 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide association study identifies novel susceptibility genes contributing to hearing impairment

Xie

Niu

Ping

et al. 2021

Preprint

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Hearing impairment (HI) is a growing public health issue of global concern, which may lead to decreased work efficiency, social withdrawal and depression. However, the knowledge of the genetic basis of HI is limited. We conducted a transcriptome-wide association study (TWAS) on risk of HI by combining an European cohort (578 cases and 583 controls) with a Chinese cohort (89 cases and 209 controls). We identified that 46 genes show transcriptome-wide significant associations with the HI risk in the meta-analyses of the European and the Chinese cohorts (all P < 3.96 ×10− 6). Of these 46 candidate genes, four genes (ARL6IP6, TMEM127, TOMM7 and JAM3) showed nominal evidence of association in both cohorts (all P < 0.05). Among these four genes, two ones (ARL6IP6 and TMEM127) revealed strong, colocalized, and potentially causal associations with HI. Pathway enrichment analyses showed that the ciliary landscape (P = 5.55 × 10− 4), cholesterol biosynthesis (P = 7.09 × 10− 3), glycerophospholipid metabolism (P = 9.65 × 10− 3) and IL-4 (P = 1.11 × 10− 2) signaling pathways are significantly associated with HI. Our findings identified several novel susceptibility genes contributing to HI, and emphasized the power of TWAS in identifying putatively causal genes for common diseases.

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“…In horses, reproductive tract dysfunction [ 199 ], reduced ovarian reserve [ 89 , 200 ], and decreased oocyte quality [ 201 , 202 , 203 , 204 ] have been well identified as age-related factors affecting fertility. Reproductive tract degeneration occurs gradually as mares grow older with oviduct lumps [ 81 ] and uterine cysts being frequently observed in old mares.…”

Section: Effects Of Maternal Environment In Equine and Humanmentioning

confidence: 99%

“…In horses, to the author’s knowledge, high throughput analysis of the oocyte is not yet available. The expression of 48 genes was analyzed in cumulus–oocyte complexes according to maternal age and showed an age-related differential expression associated with lower quality and reduced developmental competency in oocytes from old mares [ 204 ]. In vivo, the expression of a limited number of genes required for follicular and oocyte maturation was studied in the cumulus cells and the oocyte, showing asynchronous expression in old compared to young mares [ 202 ].…”

Section: Effects Of Maternal Environment In Equine and Humanmentioning

confidence: 99%

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Benammar

Derisoud

Vialard

et al. 2021

Animals

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Although there are large differences between horses and humans for reproductive anatomy, follicular dynamics, mono-ovulation, and embryo development kinetics until the blastocyst stage are similar. In contrast to humans, however, horses are seasonal animals and do not have a menstrual cycle. Moreover, horse implantation takes place 30 days later than in humans. In terms of artificial reproduction techniques (ART), oocytes are generally matured in vitro in horses because ovarian stimulation remains inefficient. This allows the collection of oocytes without hormonal treatments. In humans, in vivo matured oocytes are collected after ovarian stimulation. Subsequently, only intra-cytoplasmic sperm injection (ICSI) is performed in horses to produce embryos, whereas both in vitro fertilization and ICSI are applied in humans. Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. Embryo and oocyte cryopreservation has been mastered in humans, but not completely in horses. Finally, both species share infertility concerns due to ageing and obesity. Thus, reciprocal knowledge could be gained through the comparative study of ART and infertility treatments both in woman and mare, even though the horse could not be used as a single model for human ART.

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Expression profiles of select genes in cumulus–oocyte complexes from young and aged mares

Cited by 15 publications

References 60 publications

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Transcriptome-wide association study identifies novel susceptibility genes contributing to hearing impairment

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

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