Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds

Cheng, Yi; Liu, Songcai; Zhang, Xin; Wu, Qingyan; Li, Siming; Fu, Haoyu; Dong, Lijie; Yu, Hao; Hao, Linlin

doi:10.1007/s10930-016-9666-x

Cited by 17 publications

(16 citation statements)

References 19 publications

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“…That is to say, the gene may change its transcriptional level through alternative splicing, thereby affecting its function. In our previous studies, IGF-1R protein in Landrace pig was significantly higher than that in BaMa pig at birth (Cheng et al, 2016). However, in this study, the mRNA expression level of IGF-1R in Landrace and BaMa pigs showed no significantly different.…”

Section: Expression Of Igf-1r Iso01 and Igf-1r As02 In Different Pig contrasting

confidence: 81%

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Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs

Yang

Dong

Liu

et al. 2019

IJAR

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The transcript variants of Insulin-like growth factor 1 receptor (IGF-1R) and their expression profiles had never been illuminated in pigs until now. Herein, we identified IGF-1R AS02 as a novel splice variant of IGF-1R gene by RT-PCR and analyzed its mRNA expression level by qRT-PCR in liver, cartilage and muscle tissues, while also detecting the single-nucleotide polymorphism (SNP) site near the splice site of the IGF-1R gene (in intron 19) of BaMa and Landrace pigs. Results demonstrated that the IGF-1R AS02 variant showed a significantly (P less than 0.05) higher expression level in cartilage than in muscle and liver across two pig breeds respectively. The expression level of the normal transcript (IGF-1R ISO01) of IGF-1R in cartilage was markedly lower than that in the other two tissues (P less than 0.05). In cartilage, IGF-1R ISO01 expression was higher in BaMa than in Landrace (P less than 0.05), while the expression level of IGF-1R AS02 was lower in BaMa than in Landrace (P less than 0.05). The SNP was detected in intron 19 of the IGF-1R gene of BaMa and Landrace pigs. These results contributed to facilitating a better understanding of IGF-1R gene in pigs.

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Section: Expression Of Igf-1r Iso01 and Igf-1r As02 In Different Pig contrasting

confidence: 81%

“…In this study, BaMa pigs were chosen as the representative model of the miniature pigs and Landrace pigs (adult mean body weight, 250 kg) as large pig breeds. The two pig breeds were opposite in many growth characters but they were all genetically stable and their genetic backgrounds were clear (Cheng et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs

Yang

Dong

Liu

et al. 2019

IJAR

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“…In China, the Bama Xiang pig is a well-known miniature pig breed from Guangxi Zhuang Autonomous Region. It is characterized by highly inbred, stable heredity and mini-body size (the mean body weight of adults is nearly 40 kg) (Cheng et al, 2016;Yang et al, 2018). The Large White pig also called Yorkshire pigs, is considered the most representative of large pigs because of its large body size and high growth rate (mean body weight of adults is almost 250 kg) and is considered as the most typical representative of large pigs.…”

Section: Introductionmentioning

confidence: 99%

“…IGF-1R exhibits different protein expression levels in the liver and muscle tissues of large and miniature pigs (Cheng et al, 2016). A previous study screened nine strong linkage synonymous mutations in the coding sequence (CDS) of IGF-1R between large and miniature pigs, four of which were found in the extracellular domain (ECD), whereas five were located in the intracellular domain (ICD) (Changhong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Synonymous Mutations of Porcine Igf1r Extracellular Domain Affect Differentiation and Mineralization in MC3T3-E1 Cells

Wang

Liu

et al. 2020

Front. Cell Dev. Biol.

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Owing to the wide application of miniature pigs in biomedicine, the formation mechanism of its short stature must be elucidated. The insulin-like growth factor 1 receptor (IGF-1R), which receives signals through the extracellular domain (ECD) binding with ligands, is crucial in regulating cell growth and bone matrix mineralization. In this study, two haplotypes of Igf1r with four synonymous mutations in the coding sequences of IGF-1R ECD between large pigs (LP) and Bama pigs (BM) were stably expressed in the Igf1r-knockout MC3T3-E1 cells and named as MC3T3-LP cells (LP group) and MC3T3-BM cells (BM group), respectively. IGF-1R expression was lower in the BM group than in the LP group both in terms of transcription and translation levels, and IGF-1R expression inhibited cell proliferation. In addition, IGF-1R expression in the BM group promoted early-stage differentiation but delayed late-stage differentiation, which not only suppressed the expression of bone-related factors but also reduced alkaline phosphatase activity and calcium deposition. Moreover, different haplotypes of Igf1r changed the stability and conformation of the protein, further affecting the binding with IGF-1. Our data indicated that the four synonymous mutations of IGF1R ECD encoded by affect gene transcription and translation, thereby further leading to differences in the downstream pathways and functional changes of osteoblasts.

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“…Insulin-like growth factor-1 receptor (IGF-1R) is found on the surface of many kinds of cells, including hepatocytes, myocytes, and osteocytes (Cheng et al, 2016). Recent studies suggest that IGF-1R is involved in both glucose and bone metabolism (Pelosi et al, 2017;Solomon-Zemler et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

IGF-1R/β-catenin signaling axis is involved in type 2 diabetic osteoporosis

Zhang

Ren

Wang

et al. 2019

J. Zhejiang Univ. Sci. B

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Insulin-like growth factor-1 receptor (IGF-1R) is involved in both glucose and bone metabolism. IGF-1R signaling regulates the canonical Wnt/β-catenin signaling pathway. In this study, we investigated whether the IGF-1R/ β-catenin signaling axis plays a role in the pathogenesis of diabetic osteoporosis (DOP). Serum from patients with or without DOP was collected to measure the IGF-1R level using enzyme-linked immunosorbent assay (ELISA). Rats were given streptozotocin following a four-week high-fat diet induction (DOP group), or received vehicle after the same period of a normal diet (control group). Dual energy X-ray absorption, a biomechanics test, and hematoxylin-eosin (HE) staining were performed to evaluate bone mass, bone strength, and histomorphology, respectively, in vertebrae. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were performed to measure the total and phosphorylation levels of IGF-1R, glycogen synthase kinase-3β (GSK-3β), and β-catenin. The serum IGF-1R level was much higher in patients with DOP than in controls. DOP rats exhibited strikingly reduced bone mass and attenuated compression strength of the vertebrae compared with the control group. HE staining showed that the histomorphology of DOP vertebrae was seriously impaired, which manifested as decreased and thinned trabeculae and increased lipid droplets within trabeculae. PCR analysis demonstrated that IGF-1R mRNA expression was significantly up-regulated, and western blotting detection showed that phosphorylation levels of IGF-1R, GSK-3β, and β-catenin were enhanced in DOP rat vertebrae. Our results suggest that the IGF-1R/β-catenin signaling axis plays a role in the pathogenesis of DOP. This may contribute to development of the underlying therapeutic target for DOP.

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Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds

Cited by 17 publications

References 19 publications

Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs

Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs

Synonymous Mutations of Porcine Igf1r Extracellular Domain Affect Differentiation and Mineralization in MC3T3-E1 Cells

IGF-1R/β-catenin signaling axis is involved in type 2 diabetic osteoporosis

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