Expression profiles and potential roles of transfer RNA‐derived small RNAs in atherosclerosis

He, Xiangqin; Yang, Yanyan; Wang, Qi; Wang, Jueru; Li, Shifang; Li, Chunrong; Zong, Tingyu; Li, Xiaolu; Zhang, Ying; Zou, Yulin; Yu, Tao

doi:10.1111/jcmm.16719

Cited by 26 publications

(13 citation statements)

References 53 publications

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“…In addition, studies have found that male patients generally have higher infection and death rates than female patients (18), which is believed to be due to the influence of hormones, and the higher expression of the viral receptor, angiotensin-converting enzyme 2 (ACE2) protein in males (19). In addition, studies have reported that non-coding RNA has an important regulatory role in cardiovascular disease (20,21). As a cardiovascular regulator, ACE2 may play a potential role in the re-positive of COVID-19 and may be regulated by non-coding RNA, which is worthy of further development research.…”

Section: Discussionmentioning

confidence: 99%

Eosinophil: A Nonnegligible Predictor in COVID-19 Re-Positive Patients

Yin²,

Yang

et al. 2021

Front. Immunol.

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Although vaccine resources are being distributed worldwide, insufficient vaccine production remains a major obstacle to herd immunity. In such an environment, the cases of re-positive occurred frequently, and there is a big controversy regarding the cause of re-positive episodes and the infectivity of re-positive cases. In this case-control study, we tracked 39 patients diagnosed with COVID-19 from the Jiaodong Peninsula area of China, of which 7 patients tested re-positive. We compared the sex distribution, age, comorbidities, and clinical laboratory results between normal patients and re-positive patients, and analysed the correlation between the significantly different indicators and the re-positive. Re-positive patients displayed a lower level of serum creatinine (63.38 ± 4.94 U/L vs. 86.82 ± 16.98 U/L; P =0.014) and lower albumin (34.70 ± 5.46 g/L vs. 41.24 ± 5.44 g/L, P =0.039) at the time of initial diagnosis. In addition, two positive phases and the middle negative phase in re-positive patients with significantly different eosinophil counts (0.005 ± 0.005 × 109/L; 0.103 ± 0.033 × 109/L; 0.007 ± 0.115 × 109/L; Normal range: 0.02-0.52 × 109/L). The level of eosinophils in peripheral blood can be used as a marker to predict re-positive in patients who once had COVID-19.

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Section: Discussionmentioning

confidence: 99%

Eosinophil: A Nonnegligible Predictor in COVID-19 Re-Positive Patients

Yin²,

Yang

et al. 2021

Front. Immunol.

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“…Compared to miRNAs, tsRNAs are novel and remain more unknown. It has been reported that the tsRNA tRF-Gly-GCC is involved in the pathogenesis of atherosclerosis via functional regulation in human umbilical vein endothelial cells and vascular smooth muscle cells ( 23 ). Until now, few studies have investigated the role of tsRNAs in ocular disorders.…”

Section: Discussionmentioning

confidence: 99%

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

et al. 2022

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PurposeWe sought to reveal the expression profiles of transfer RNA-derived small RNAs (tsRNAs) and microRNAs (miRNAs) in the vitreous humor of patients with proliferative diabetic retinopathy (PDR).MethodsVitreous humor samples were obtained from PDR patients and a control group for this study. Sequencing of small RNAs was conducted to assess the expression profiles of tsRNAs and miRNAs in both groups, which was followed by validation using reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Bioinformatics analyses were conducted to predict the target genes and their potential biological functions and signaling pathways.ResultsA total of 37 tsRNAs and 70 miRNAs with significant differences were screened out from the vitreous humor samples of PDR patients compared to controls. Following validation by RT-qPCR, the target genes of the validated tsRNAs and miRNAs were predicted, and Gene Ontology analysis indicated that the target genes of the tsRNAs were most enriched in the cellular macromolecule metabolic process, cytoplasm, and ion-binding, while those of the miRNAs were most abundant in the regulation of major metabolic process, cytoplasm, and protein-binding. In addition, Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the target genes of said tsRNAs and miRNAs were most enriched in the adenosine monophosphate-activated protein kinase signaling pathway and Th17 cell differentiation, respectively.ConclusionsThe present study identified altered tsRNAs and miRNAs in vitreous humor samples of PDR patients, which may play important roles in the pathogenesis of PDR and could be considered potential therapeutic targets in the treatment of PDR.

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“…tRF GlnCTG , which is highly increased in injured rat common carotid arteries, promotes rat VSMC proliferation by inhibiting Fas cell surface death receptor (FAS) levels in a 3′-UTR-targeted manner [ 8 ]. tRF-Gly-GCC is increased in patients with atherosclerosis and promotes VSMC proliferation [ 9 ]. 5′-tiRNA-Cys-GCA, which is decreased in human and mouse aortic dissection, inhibits VSMC proliferation by reducing STAT4 levels in a 3′-UTR-targeted manner [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the roles of tsRNAs in mediating cardiovascular diseases by regulating VSMC proliferation have been gradually revealed recently [ 8 – 10 ]. For example, tRF GlnCTG and tRF-Gly-GCC promote VSMC proliferation [ 8 , 9 ], whereas 5′-tiRNA-Cys-GCA inhibits VSMC proliferation, migration, and dedifferentiation by reducing the levels of its target gene signal transducer and activator of transcription 4 (STAT4) [ 10 ]. However, the actual repertoire of tsRNAs affecting VSMC proliferation remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells

Zhao

Lin

et al. 2022

Cell Mol Biol Lett

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Background Abnormal proliferation of vascular smooth muscle cells (VSMCs) contributes to vascular remodeling diseases. Recently, it has been discovered that tRNA-derived small RNAs (tsRNAs), a new type of noncoding RNAs, are related to the proliferation and migration of VSMCs. tsRNAs regulate target gene expression through miRNA-like functions. This study aims to explore the potential of tsRNAs in human aortic smooth muscle cell (HASMC) proliferation. Methods High-throughput sequencing was performed to analyze the tsRNA expression profile of proliferative and quiescent HASMCs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the sequence results and subcellular distribution of AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076. Based on the microRNA-like functions of tsRNAs, we predicted target promoters and mRNAs and constructed tsRNA–promoter and tsRNA–mRNA interaction networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to reveal the function of target genes. EdU incorporation assay, Western blot, and dual-luciferase reporter gene assay were utilized to detect the effects of tsRNAs on HASMC proliferation. Results Compared with quiescent HASMCs, there were 1838 differentially expressed tsRNAs in proliferative HASMCs, including 887 with increased expression (fold change > 2, p < 0.05) and 951 with decreased expression (fold change < ½, p < 0.05). AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076 were increased in proliferative HASMCs and were mainly located in the nucleus. Bioinformatics analysis suggested that the four tsRNAs involved a variety of GO terms and pathways related to VSMC proliferation. AS-tDR-000067 promoted HASMC proliferation by suppressing p53 transcription in a promoter-targeted manner. AS-tDR-000076 accelerated HASMC proliferation by attenuating mitofusin 2 (MFN2) levels in a 3′-untranslated region (UTR)-targeted manner. Conclusions During HASMC proliferation, the expression levels of many tsRNAs are altered. AS-tDR-000067 and AS-tDR-000076 act as new factors promoting VSMC proliferation.

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Expression profiles and potential roles of transfer RNA‐derived small RNAs in atherosclerosis

Cited by 26 publications

References 53 publications

Eosinophil: A Nonnegligible Predictor in COVID-19 Re-Positive Patients

Eosinophil: A Nonnegligible Predictor in COVID-19 Re-Positive Patients

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells

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