Expression Profiles and Post-Translational Modifications of Phosphoenolpyruvate Carboxylase Isozymes of Bienertia sinuspersici during Leaf Development

Caburatan, Lorrenne; Kim, J.; Park, J.

doi:10.1134/s1021443719050042

Cited by 4 publications

(10 citation statements)

References 29 publications

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“…The suggestion that PEPC3 is a non-photosynthetic enzyme is consistent with the data of Voznesenskaya and colleagues [14], who confirmed the presence of Rubisco as a potential photosynthetic agent in the early stage of leaf development in B. sinuspersici. This information was further verified by Caburatan et al [13], who showed that upon differentiation into the peripheral compartment (PC) and central compartment (CC), Rubisco in mature leaves of B. sinuspersici highly accumulated in the CC, which has more primitive cells than those in the PC, right before the compartmentalization occurred.…”

Section: Differential Expression Of Pepcs At Different Stages Of Plant Developmentmentioning

confidence: 62%

“…PEPC distribution can vary in different tissues and in specific areas of a particular tissue. As in the case of PEPC4 of B. sinuspersici, a 107-kDa PEPC (p107) was detected on immunoblots of extracts from young, intermediate, and mature leaves and was found to be more apparent in mature leaf samples [13].…”

Section: Tissue-specific Distribution Of C4 and C3 Pepcsmentioning

confidence: 65%

“…Furthermore, immunoblotting of extracts obtained from three regions of mature leaves, namely, the base, middle part, and tip, showed that the p107 PEPC polypeptide was highly expressed in the tip portion of the mature leaf but not in the base and middle portion [13]. These findings could be due to an earlier maturation of the tip portion of the leaf than that of its lower part, indicating that PEPC expression is higher in mature leaves than in earlier ones.…”

Section: Tissue-specific Distribution Of C4 and C3 Pepcsmentioning

confidence: 91%

“…In the C 4 plant B. sinuspersici, both PTMs have been reported to be conserved in all PEPC isozymes [13]. Phosphorylation of the 107-kDa PEPC in the tip portion of the mature leaf was shown to be more prominent than that at the base or in the middle portion of mature leaves.…”

Section: Posttranslational Modifications (Ptms): Phosphorylation and Monoubiquitination Of Pepcsmentioning

confidence: 97%

“…Accordingly, the differential expression of genes encoding C 4 -cycle enzymes, such as PEPC, is largely due to transcriptional control [9,11,12]. In the single-cell C 4 species Bienertia sinuspersici, differential expression of PEPC isozymes has been observed at different developmental stages [13]. Analysis of relative mRNA expression of PEPC isoforms in extracts from young, intermediate, and mature leaves of B. sinuspersici showed that PEPC3 was predominantly expressed in the early stage, while PEPC1 and PEPC2 were expressed in the intermediate and mature stages of leaf development, respectively (Figure S1).…”

Section: Differential Expression Of Pepcs At Different Stages Of Plant Developmentmentioning

confidence: 99%

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Differential Expression, Tissue-Specific Distribution, and Posttranslational Controls of Phosphoenolpyruvate Carboxylase

Caburatan

Park

2021

Plants

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Phosphoenolpyruvate carboxylase (PEPC) is a ubiquitous cytosolic enzyme, which is crucial for plant carbon metabolism. PEPC participates in photosynthesis by catalyzing the initial fixation of atmospheric CO2 and is abundant in both C4 and crassulacean acid metabolism leaves. PEPC is differentially expressed at different stages of plant development, mostly in leaves, but also in developing seeds. PEPC is known to show tissue-specific distribution in leaves and in other plant organs, such as roots, stems, and flowers. Plant PEPC undergoes reversible phosphorylation and monoubiquitination, which are posttranslational modifications playing important roles in regulatory processes and in protein localization. Phosphorylation activates the PEPC enzyme, making it more sensitive to glucose-6-phosphate and less sensitive to malate or aspartate. PEPC phosphorylation is known to be diurnally regulated and delicately changed in response to various environmental stimuli, in addition to light. PEPCs belong to a small gene family encoding several plant-type and distantly related bacterial-type PEPCs. This paper provides a minireview of the general information on PEPCs in both C4 and C3 plants.

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Section: Differential Expression Of Pepcs At Different Stages Of Plant Developmentmentioning

confidence: 62%

Section: Tissue-specific Distribution Of C4 and C3 Pepcsmentioning

confidence: 65%

Section: Tissue-specific Distribution Of C4 and C3 Pepcsmentioning

confidence: 91%

Section: Posttranslational Modifications (Ptms): Phosphorylation and Monoubiquitination Of Pepcsmentioning

confidence: 97%

Section: Differential Expression Of Pepcs At Different Stages Of Plant Developmentmentioning

confidence: 99%

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Differential Expression, Tissue-Specific Distribution, and Posttranslational Controls of Phosphoenolpyruvate Carboxylase

Caburatan

Park

2021

Plants

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Multifaceted functions of post-translational enzyme modifications in the control of plant glycolysis

O’Leary

Plaxton

2020

Current Opinion in Plant Biology

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C4 Phosphoenolpyruvate Carboxylase: Evolution and transcriptional regulation

Carvalho,

Gomes,

Saibo

2023

Genet. Mol. Biol.

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Photosynthetic phosphoenolpyruvate carboxylase (PEPC) catalyses the irreversible carboxylation of phosphoenolpyruvate (PEP), producing oxaloacetate (OAA). This enzyme catalyses the first step of carbon fixation in C 4 photosynthesis, contributing to the high photosynthetic efficiency of C 4 plants. PEPC is also involved in replenishing tricarboxylic acid cycle intermediates, such as OAA, being involved in the C/N balance. In plants, PEPCs are classified in two types: bacterial type (BTPC) and plant-type (PTPC), which includes photosynthetic and non-photosynthetic PEPCs. During C 4 evolution, photosynthetic PEPCs evolved independently. C 4 PEPCs evolved to be highly expressed and active in a spatial-specific manner. Their gene expression pattern is also regulated by developmental cues, light, circadian clock as well as adverse environmental conditions. However, the gene regulatory networks controlling C 4 PEPC gene expression, namely its cell-specificity, are largely unknown. Therefore, after an introduction to the evolution of PEPCs, this review aims to discuss the current knowledge regarding the transcriptional regulation of C 4 PEPCs, focusing on cell-specific and developmental expression dynamics, light and circadian regulation, as well as response to abiotic stress. In conclusion, this review aims to highlight the evolution, transcriptional regulation by different signals and importance of PEPC in C 4 photosynthesis and its potential as tool for crop improvement.

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Expression Profiles and Post-Translational Modifications of Phosphoenolpyruvate Carboxylase Isozymes of Bienertia sinuspersici during Leaf Development

Cited by 4 publications

References 29 publications

Differential Expression, Tissue-Specific Distribution, and Posttranslational Controls of Phosphoenolpyruvate Carboxylase

Differential Expression, Tissue-Specific Distribution, and Posttranslational Controls of Phosphoenolpyruvate Carboxylase

Multifaceted functions of post-translational enzyme modifications in the control of plant glycolysis

C4 Phosphoenolpyruvate Carboxylase: Evolution and transcriptional regulation

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