Expression profiles and functional associations of endogenous androgen receptor and caveolin-1 in prostate cancer cell lines

Bennett, Nigel C.; Hooper, John D.; Johnson, David W.; Gobé, Glenda C.

doi:10.1002/pros.22767

Cited by 15 publications

(16 citation statements)

References 36 publications

(53 reference statements)

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“…The latter requires no direct AR functional involvement. In line with previous published results, this study showed that PC3 and DU145 cell lines didn't express AR [34][35][36]. Therefore, possible molecular changes found in either PC3 or DU145 cell lines may represent the AR-independent mechanisms.…”

Section: Discussionsupporting

confidence: 90%

Identification of Mutations, Expression Alterations and Fusion Transcripts by Next Generation RNAseq in Castration-Resistant Prostate Cancer Cell lines with Possible Clinical Relevance

Ma¹,

Miao²,

Peng³

et al. 2017

Next Generat Sequenc & Applic

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Androgen Deprivation Therapy (ADT) would benefit prostate cancer patients initially but cancer cells can eventually develop castration resistance. In this study, we compared androgen-dependent and androgenindependent cell lines to find potential genes associated with acquired resistance to ADT. Using RNAseq, we found 4397 mutations distributed in 2579 genes, out of which, 1574 mutated genes could also be found in prostate cancer tumor samples collected in Cosmic database (http://cancer.sanger.ac.uk/cosmic). We also discovered 157 and 549 genes which were down and up-regulated respectively in both PC3 and DU145 compared to LNCaP. Network analysis resulted in 3 dominant connection notes: GCR/MCR (NR3C1) and PKA-cat kinase (PRKACB) and PKC family (PRKD1). By ChimeraScan analysis, 48, 117 and 60 chimeric transcripts were discovered in DU145, LNCaP and PC3 respectively. Among them, six predicted fusions expressed specifically in androgen-independent cell lines (DU145 and PC3). Some of these gene mutations and transcription alterations have been reported in tumor samples from prostate cancer patients and may have certain associations with acquired resistance to anti-hormone therapy in prostate cancer. A proportion of mutations are enriched in genes involved in immune response pathways, suggesting new targets to develop effective treatments to overcome castration resistance.

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Section: Discussionsupporting

confidence: 90%

Identification of Mutations, Expression Alterations and Fusion Transcripts by Next Generation RNAseq in Castration-Resistant Prostate Cancer Cell lines with Possible Clinical Relevance

Ma¹,

Miao²,

Peng³

et al. 2017

Next Generat Sequenc & Applic

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“…A strong correlation was found between caveolin-1 expression and the presence of venous invasion of HCC samples ( P = 0.02) [20]. In fact, in a series of studies in vivo researchers found caveolin-1 upregulation in several tumors, such as prostate cancer [23], lung cancer [24], and breast cancer [22]. Moreover, some recent papers report caveolin-1 overexpression in HCC and its correlation with prognostic factors [21].…”

Section: Discussionmentioning

confidence: 99%

Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma

Liu¹,

Lv²,

Shi³

et al. 2014

BioMed Research International

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Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival.

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“…Previous studies showed that Cav-1 is involved in various signaling pathways including estrogen receptor, epidermal growth factor receptor (EGFR), Her2/neu, tumor growth factor-β (TGFβ), and mTOR[ 9 ], and serves as an oncogene in lung, breast, and ovarian cancers [ 10 – 12 ]. In contrast, Cav-1 plays the role of a tumor suppressor in prostate and bladder cancers [ 13 , 14 ]. Bender et al [ 15 ] confirmed that Cav-1 was downregulated in patients with colon cancer, while other studies revealed that Cav-1 was overexpressed in patients with colon adenocarcinoma [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Caveolin-1 Inhibits Proliferation, Migration, and Invasion of Human Colorectal Cancer Cells by Suppressing Phosphorylation of Epidermal Growth Factor Receptor

et al. 2018

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BackgroundAlthough downregulation of caveolin-1 (Cav-1), which is a key constituent of membrane caveolae and a regulator of cellular processes, is associated with colorectal cancer (CRC), its involvement in the disease progression is largely unknown. This study aimed to explore the role of Cav-1 in CRC and the associated mechanisms.Material/MethodsFresh tissues from patients with CRC and human CRC SW480 cells were used to evaluate Cav-1 and Ki-67 expression using immunohistochemistry and Western blotting. The MTS and Transwell assays were performed to determine the effects of Cav-1 overexpression via pcDNA3.1/Cav-1 plasmid on cell proliferation and metastasis. The effect of Cav-1 on the epidermal growth factor receptor (EGFR) activation was evaluated by Western blotting. The correlation of Cav-1 expression with clinicopathological factors was statistically analyzed.ResultsOverexpression of Cav-1 significantly reduced proliferation, migration, and invasion of SW480 cancer cells in vitro. The EGF-induced phosphorylation of EGFR and activations of the RAF-MEK-ERK and PI3K-AKT pathways were adversely regulated by Cav-1 overexpression in vitro. In 76 cases of CRC patients with EGFR expression, a negative correlation was observed between the level of Cav-1 and tumor-node-metastasis stage, lymph node metastasis, and distant metastasis (All p<0.05). Finally, the expression level of Cav-1 was negatively correlated with that of Ki-67.ConclusionsThis report is the first to show that overexpression of Cav-1significantly inhibits the proliferation, migration, and invasion potential of SW480 cells, possibly through reducing EGF-induced EGFR activation. High Cav-1 expression level may be a predictor of positive outcomes in patients with colorectal cancer.

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Expression profiles and functional associations of endogenous androgen receptor and caveolin-1 in prostate cancer cell lines

Abstract: These data show endogenous AR and Cav-1 mRNA and protein expression is inversely related in PCa cells, with Cav-1 acting on the androgen/AR signaling axis possibly as an AR co-activator, demonstrated by diminished AR genomic activity following Cav-1 knockdown.

Cited by 15 publications

References 36 publications

Identification of Mutations, Expression Alterations and Fusion Transcripts by Next Generation RNAseq in Castration-Resistant Prostate Cancer Cell lines with Possible Clinical Relevance

Identification of Mutations, Expression Alterations and Fusion Transcripts by Next Generation RNAseq in Castration-Resistant Prostate Cancer Cell lines with Possible Clinical Relevance

Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma

Caveolin-1 Inhibits Proliferation, Migration, and Invasion of Human Colorectal Cancer Cells by Suppressing Phosphorylation of Epidermal Growth Factor Receptor

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