Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

ShuHui, Fu; Li, Fangyuan; Yan, Xin; Hao, Chi

doi:10.1093/jisesa/ieaa107

Cited by 9 publications

(13 citation statements)

References 88 publications

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“…Previous studies showed that OBP genes were highly expressed in insect heads, suggesting that they contribute to the detection of host plant volatiles and sex pheromones (Chang et al, 2015;Liu et al, 2015;Wang et al, 2016). The following studies also showed that the indicated CSPs are highly expressed in the male antennae of the following insects: CSP11 of Plutella xylostella (Fu et al, 2020), CSP8 of Nilaparvata lugens (Waris et al, 2018), CSP1 of Cylas formicarius (Hua et al, 2021), and CSP3 of Cnaphalocrocis medinalis (Zeng et al, 2018). High expression of CSP in male antennae suggests that they may be more involved in the perception of sex pheromones than plant volatiles.…”

Section: Discussionmentioning

confidence: 98%

Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Yang

Hua²,

Zhu³

et al. 2022

Front. Physiol.

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Bradysia odoriphaga (Diptera: Sciaridae) is a serious pest of Chinese chives cultivated in China. Chemosensory proteins (CSPs) are important components of insect olfactory systems that capture and bind environmental semiochemicals which are then transported to olfactory receptors. Despite their importance, the mechanism of olfaction and related behavioral processes in B. odoriphaga have not been characterized. Here, we found that BodoCSP4 has an important olfactory function. RT-qPCR indicated that BodoCSP4 expression was highest in the heads (antennae removed) of adult males, followed by the antennae of adult males. Competitive binding assays with 33 ligands indicated that BodoCSP4 binds well with methyl allyl disulfide, diallyl disulfide, and n-heptadecane; the corresponding dissolution constants (Ki) were as high as 5.71, 5.71, and 6.85 μM, respectively. 3D-structural and molecular docking indicated that BodoCSP4 has five α-helices and surrounds the ligand with certain hydrophobic residues including Leu60, Leu63, Leu64, Ala67, Val28, Ile30, Ile33, Leu34, and Val86, suggesting these residues help BodoCSP4 bind to ligands. Silencing of BodoCSP4 significantly decreased the attraction of B. odoriphaga males to diallyl disulfide and n-heptadecane but not to methyl allyl disulfide in Y-tube olfaction assays. These results increase our understanding of how BodoCSP4 contributes to host and female localization by B. odoriphaga males.

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Section: Discussionmentioning

confidence: 98%

Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Yang

Hua²,

Zhu³

et al. 2022

Front. Physiol.

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“…The compound 2,4-dimethylheptane also elicited strong EAG and behavioural responses in P. xylostella. Fu et al (2020) found F I G U R E 2 Gas chromatography coupled with electroantennographic detection (GC-EAD) responses of female adults of Plutella xylostella to headspace volatiles from wounded Mentha spicata. The four components with electroantennographic detectionactivity were confirmed to be 2,4-dimethyl-heptane, 4-methyloctane, 2-methylnonane and myrcene by gas chromatography-mass spectrometry.…”

Section: Discussionmentioning

confidence: 99%

Electroantennogram and behavioural responses of Plutella xylostella to volatiles from the non‐host plant Mentha spicata

Yan

Wang

et al. 2023

J Applied Entomology

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Plutella xylostella Linnaeus (Lepidoptera: Plutellidae) is one of the most important pests of cruciferous vegetables. Mechanically damaged Mentha spicata Linnaeus (Lamiales: Lamiaceae) strongly repels P. xylostella adults; however, the repellent mechanism remains unclear. In this study, the repellent mechanism was explored based on the electroantennogram (EAG) and behavioural responses of P. xylostella to volatiles from the damaged M. spicata. First, the active volatile compounds in the headspace volatiles of damaged M. spicata were identified by gas chromatography‐electroantennographic detection (GC‐EAD) and gas chromatography–mass spectrometry (GC–MS). Furthermore, the EAG and behavioural responses of P. xylostella to these compounds were investigated. The GC‐EAD results indicated that four compounds, 2,4‐dimethylheptane, 4‐methyloctane, 2‐methylnonane, and myrcene induced EAG responses in female antennae. 2‐methylnonane and myrcene elicited strong dose‐dependent EAG responses starting at a dose of 1 μg/μL. Choice tests in a four‐arm olfactometer showed significant repellent effects of the four compounds on P. xylostella adults at 100 μg/μL, and the repellent indices of 2,4‐dimethylheptane, 4‐methyloctane, 2‐methylnonane, and myrcene were 70.0%, 76.7%, 66.7%, and 73.3% to mated females, respectively. Finally, oviposition repellence assays showed that female adults were significantly repelled by these compounds, and the oviposition repellent index for myrcene was 23.9%. 2,4‐dimethylheptane, 4‐methyloctane, 2‐methylnonane, and myrcene mediate the repellence of wounded M. spicata to P. xylostella adults, suggesting that the four compounds contribute towards developing behavioural regulators of this important pest.

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“…We next conducted the binding experiments with several candidate ligand molecules. These molecules have been demonstrated to bind to different CSP proteins in the literature [ 8 , 12 , 27 , 28 , 29 ]. Our results showed that the SlCSP8 protein has the highest and lowest affinities to rhodojaponin III and avermectin, respectively, while it displays moderate affinities to 12-bromododecanol and bombykol, with the former being a better binder than the latter.…”

Section: Discussionmentioning

confidence: 99%

The Crystal Structure of the Spodoptera litura Chemosensory Protein CSP8

Jia

Zeng

Zhang

et al. 2021

Insects

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Spodoptera litura F. is a generalist herbivore and one of the most important economic pests feeding on about 300 host plants in many Asian countries. Specific insect behaviors can be stimulated after recognizing chemicals in the external environment through conserved chemosensory proteins (CSPs) in chemoreceptive organs, which are critical components of the olfactory systems. To explore its structural basis for ligand-recognizing capability, we solved the 2.3 Å crystal structure of the apoprotein of S. litura CSP8 (SlCSP8). The SlCSP8 protein displays a conserved spherical shape with a negatively charged surface. Our binding assays showed that SlCSP8 bound several candidate ligands with differential affinities, with rhodojaponin III being the most tightly bound ligand. Our crystallographic and biochemical studies provide important insight into the molecular recognition mechanism of the sensory protein SlCSP8 and the CSP family in general, and they suggest that CSP8 is critical for insects to identify rhodojaponin III, which may aid in the CSP-based rational drug design in the future.

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Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Cited by 9 publications

References 88 publications

Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Electroantennogram and behavioural responses of Plutella xylostella to volatiles from the non‐host plant Mentha spicata

The Crystal Structure of the Spodoptera litura Chemosensory Protein CSP8

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