Expression profiles and associations of adiponectin and adiponectin receptors with intramuscular fat in Tibetan chicken

Abstract

1. Adiponectin and its receptors (ADIPOR1 and ADIPOR2) are novel endocrine systems that act at various levels to modulate glucose and lipid metabolism. This study was designed to investigate the spatial expression of adiponectin, ADIPOR1 and ADIPOR2 genes in various tissues in Tibetan chicken. The temporal expression of adiponectin and its receptor mRNAs were also studied in adipose tissue, breast muscle and thigh muscle and the correlations of the levels of adiponectin, ADIPOR1 and ADIPOR2 mRNA with the contents of intramuscular fat in breast muscle and thigh muscle of Tibetan chicken were determined. 2. Quantitative real-time PCR detected chicken adiponectin, ADIPOR1 and ADIPOR2 mRNA transcripts in heart, liver, spleen, lung, kidney, skeletal muscle and adipose tissue. 3. Adipose tissue contained the highest amount of adiponectin mRNA followed by the kidney and liver. The expression levels of ADIPOR1 mRNA were significantly higher in adipose tissue, lung and spleen, and adipose tissue exhibited significantly higher levels of ADIPOR2 mRNA followed by the spleen and lung compared with other tissues. 4. Temporal expression profiles of adiponectin, ADIPOR1 and ADIPOR2 mRNA showed gender differences in adipose tissue and skeletal muscle at certain ages. In adipose tissue, adiponectin mRNA was higher in 154-d-old females and ADIPOR1 mRNA was higher in 154-d-old males: Adiponectin and ADIPOR2 mRNA were higher, and ADIPOR1 mRNA was lower, in thigh muscle in female compared with male chickens. 5. The correlation data showed that, except for adiponectin mRNA, the levels of ADIPOR1 and ADIPOR2 mRNA in thigh muscle of males were significantly positively correlated with IMF (r = 0.206 for the ADIPOR1 gene and r = 0.676 for the ADIPOR2 gene). 6. Taken together, it was concluded that adiponectin and the ADIPOR1 and ADIPOR2 genes are ubiquitously expressed in various tissues of Tibetan chicken and the expression of the adiponectin system is gender-dependant at certain ages in adipose tissue and skeletal muscle.

Chicken Is a Useful Model to Investigate the Role of Adipokines in Metabolic and Reproductive Diseases

“…Similarly, the chicken ADIPOR2 cDNA was 76–78% homologous to human, mouse, or pig ADIPOR2 cDNA, while the deduced protein sequence was 82% similar to mammalian ADIPOR2 [ 122 ]. Adiponectin and adiponectin receptor genes are ubiquitously expressed in various tissues ( Table 1 ) [ 123 , 124 ], and the expression of the adiponectin system (adiponectin, ADIPOR1, and ADIPOR2) in adipose tissue and muscle depends on the gender and age of the animals [ 125 ]. In adipose tissue, adiponectin mRNA was higher in 154-day-old females than in males and ADIPOR1 mRNA was higher in 154-day-old males than in females.…”

Adiponectin modulates steroid hormone secretion, granulosa cell proliferation and apoptosis via binding its receptors during hens’ high laying period

“…It was also discovered that Adiponectin receptor agonists (AdipoRon) could activate the expression of adiponectin and then limit the occurrence of these metabolic diseases and inflammatory responses via regulating AMPK and PPARα signaling pathways ( Yamauchi et al., 2003 ; Kita et al., 2019 ; Adiyaman et al., 2020 ). In recent years, a number of studies have reported that adiponectin and its receptors are widely present in various tissues and cell types, including the reproductive tissues of human and animals ( Lord et al., 2005 ; Nigro et al., 2013 ; Smolinska et al., 2014 ; Zhang et al., 2017 ), which indicated that it may be involved in multiple physiological processes, especially the reproductive process ( Chabrolle et al., 2007 ; Maillard et al., 2010 ; Kamil et al., 2018 ). For example, it has been proved that AdipoRon fed mice could experience cardiac hypertrophy and fibrosis caused by over-pressure in remission, which implied to affect by the activation of the AMPK signaling pathway ( Ning et al., 2018 ).…”

Comparison of liver transcriptome from high- and low-intramuscular fat Chaohu ducks provided additional candidate genes for lipid selection

“…Chen et al (2017) also found that the highest levels of PPAR-γ are expressed at approximately 150 days in female Wuhua chickens and positively correlated with IMF (r = 0.875, P = 0.065). The mRNA expression levels of ADIPOR1 and ADIPOR2 genes in the thigh muscle of male Tibetan chickens are significantly positively correlated with IMF (Zhang et al 2017). The expression of A-FABP gene is also positively associated with IMF content in chickens (Wang et al 2017).…”